Effects of low temperature on electrophysiology and mechanophysiology of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
Tóm tắt
Studies related to low temperature and their effect on cardiomyocytes are essential as hypothermia—like situations have been known to induce arrhythmia or ventricular fibrillation. Till date, several studies have been carried out on animals and their electrophysiological responses have been studied in the form of action potential. However, for a complete assessment of the effect of low temperature, mechanophysiological changes along with electrophysiological changes need to be investigated, at the tissue level. In this study, the effect of culture temperature on cell growth has been studied by measuring the field potential and contractility of human induced pluripotent stem cell-derived cardiomyocytes. This study has the potential to further improve the understanding of low temperature on human cells.
Tài liệu tham khảo
Kaplan JA, Wells PH (1982) Electrocardiographic monitoring. In: Ream AK, Fogdall RP (eds) Acute cardiovascular management anesthesia and intensive care. Lippincott, Toronto, pp 151–205
Biorck G, Johansson BW (1955) Comparative studies on temperature effects upon electrocardiogram in some vertebrates. Acta Physiol Scand 34:257–272
Johansson BW, BiBrck G, Haeger K, Sjiistrtim B (1956) Electrocardiographic observations on patients operated upon in hypothermia. Acta Med Scand 155:257–269
Steif PS, Palastro MC, Rabin Y (2007) The effect of temperature gradients on stress development during cryopreservation via vitrification. Cell Preserv Technol 5(2):104–115
Kiyosue T, Arita M, Muramatsu H, Spindler AJ, Noble D (1993) Ionic mechanisms of action potential prolongation at low temperature in guinea-pig ventricular myocytes. J Physiol 468(1):85–106
Halbach M, Egert U, Hescheler J, Banach K (2003) Estimation of action potential changes from field potential recordings in multicellular mouse cardiac myocyte cultures. Cell Physiol Biochem 13(5):271–284
Smith AS, Choi E, Gray K, Macadangdang J, Ahn EH, Clark EC, Laflamme MA, Wu JC, Murry CE, Tung L, Kim DH (2019) NanoMEA: a tool for high-throughput, electrophysiological phenotyping of patterned excitable cells. Nano Lett 20(3):1561–1570
Liu B, Wang LCH, Belke DD (1991) Effect of low temperature on the cytosolic free Ca2+ in rat ventricular myocytes. Cell calcium 12(1):11–18
Souza MMD, Boyle RT (2001) A moderate decrease in temperature inhibits the calcium signaling mechanism (s) of the regulatory volume decrease in chick embryo cardiomyocytes. Braz J Med Biol Res 34:137–141
Galli GL, Lipnick MS, Shiels HA, Block BA (2011) Temperature effects on Ca2+ cycling in scombrid cardiomyocytes: a phylogenetic comparison. J Exp Biol 214(7):1068–1076
Fu Y, Zhang GQ, Hao XM, Wu CH, Chai Z, Wang SQ (2005) Temperature dependence and thermodynamic properties of Ca2+ sparks in rat cardiomyocytes. Biophys J 89(4):2533–2541
Liu B, Wohlfart B, Johansson BW (1990) Effects of low temperature on contraction in papillary muscles from rabbit, rat, and hedgehog. Cryobiology 27(5):539–546
Wang S, Zhou Z, Qian H (1999) Temperature dependence of intracellular free calcium in cardiac myocytes from rat and ground squirrel measured by confocal microscopy. Sci China Ser C Life Sci 42(3):293–299