Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Ảnh hưởng của nồng độ amoni cao đến sự phát triển và hình thành anthocyanin trong dịch treo tế bào nho (Vitis vinifera L.) được nuôi cấy trong môi trường sản xuất
Tóm tắt
Việc nuôi cấy dịch treo tế bào Vitis vinifera trong môi trường sản xuất có đặc trưng là nồng độ sucrose cao và nồng độ nitrate thấp (132 mM và 6.25 mM tương ứng) đã ức chế sự phát triển nhưng lại làm tăng tích lũy nội bào của anthocyanin, đặc biệt là peonidin 3-glucoside. Việc tăng nồng độ amoni trong môi trường sản xuất từ 2 đến 8–16 mM làm tăng sự phát triển và giảm tích lũy anthocyanin, đặc biệt là peonidin 3-glucoside. Thay vào đó, peonidin 3-p-coumaroylglucoside đã tích lũy. Tại nồng độ amoni 24 mM, sự phát triển bị ức chế và việc tích lũy peonidin 3-p-coumaroylglucoside là đáng kể (p<0.05) và chiếm 42% tổng số anthocyanin sau 12 ngày nuôi cấy, so với 19% trong môi trường sản xuất có 2 mM amoni.
Từ khóa
#Vitis vinifera #dịch treo tế bào #anthocyanin #peonidin #nồng độ amoni #môi trường sản xuấtTài liệu tham khảo
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