Effects of dietaryn-6:n-3 PUFA ratio on fatty acid composition, free amino acid profile and gene expression of transporters in finishing pigs

British Journal of Nutrition - Tập 113 Số 5 - Trang 739-748 - 2015
Fengna Li1, Yehui Duan1,2, Yinghui Li2, Yulong Tang1, Mei Geng1, A.O. Oso3,1, Sung Woo Kim4, Yulong Yin1,5
1Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha Hunan 410125, People's Republic of China
2University of Chinese Academy of Sciences, Beijing 100039, People's Republic of China
3Animal Nutrition Department, College of Animal Science and Livestock Production, Federal University of Agriculture, PMB 2240 Abeokuta, Ogun State, Nigeria
4Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA
5Southwest Collaborative Innovation Center of Swine for Quality & Safety, #211 Huiming Road, Wenjiang District, Chengdu, People's Republic of China

Tóm tắt

Revealing the expression patterns of fatty acid and amino acid transporters as affected by dietaryn-6:n-3 PUFA ratio would be useful for further clarifying the importance of the balance betweenn-6 andn-3 PUFA. A total of ninety-six finishing pigs were fed one of four diets with the ratio of 1:1, 2·5:1, 5:1 and 10:1. Pigs fed the dietaryn-6:n-3 PUFA ratio of 5:1 had the highest (P< 0·05) daily weight gain, and those fed the dietaryn-6:n-3 PUFA ratio of 1:1 had the largest loin muscle area (P< 0·01). The concentration ofn-3 PUFA was raised as the ratio declined (P< 0·05) in the longissimus dorsi and subcutaneous adipose tissue. The contents of tryptophan, tasty amino acids and branched-chain amino acids in the longissimus dorsi were enhanced in pigs fed the dietaryn-6:n-3 PUFA ratios of 1:1–5:1. The mRNA expression level of the fatty acid transporter fatty acid transport protein-1 (FATP-1) was declined (P< 0·05) in the longissimus dorsi of pigs fed the dietaryn-6:n-3 PUFA ratios of 1:1–5:1, and increased (P< 0·05) in the subcutaneous adipose tissue of pigs fed the dietaryn-6:n-3 PUFA ratios of 5:1 and 10:1. The expression profile ofFATP-4was similar to those ofFATP-1in the adipose tissue. The mRNA expression level of the amino acid transceptorsLAT1andSNAT2was up-regulated (P< 0·05) in the longissimus dorsi of pigs fed the dietaryn-6:n-3 PUFA ratios of 1:1 and 2·5:1. In conclusion, maintaining the dietaryn-6:n-3 PUFA ratios of 1:1–5:1 would facilitate the absorption and utilisation of fatty acids and free amino acids, and result in improved muscle and adipose composition.

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Tài liệu tham khảo

10.1152/japplphysiol.01408.2010

10.1016/j.brainres.2005.01.008

10.1016/j.bcp.2008.10.020

10.2741/3798

10.1016/j.bbamem.2012.10.006

10.1007/s00726-010-0612-5

10.1186/2193-1801-2-483

10.1007/s00726-008-0155-1

10.1016/j.jnutbio.2009.01.004

10.2337/db06-0822

10.1074/jbc.M211412200

10.1194/jlr.M700166-JLR200

10.3945/jn.114.192120

Bonen, 2007, Is membrane transport of FFA mediated by lipid, protein, or both? Mechanisms and regulation of protein-mediated cellular fatty acid uptake: molecular, biochemical, and physiological evidence (invited review), Physiology (Bethesda), 22, 15

10.1016/S0083-6729(08)60895-X

10.1194/jlr.M600441-JLR200

10.1371/journal.pone.0088335

10.1242/dev.01821

10.1007/s00726-010-0538-y

10.3945/ajcn.110.005611

Rennie, 2006, Branched-chain amino acids as fuels and anabolic signals in human muscle, J Nutr, 136, 264S, 10.1093/jn/136.1.264S

10.1074/jbc.M306575200

10.3945/ajcn.110.006643

10.1016/j.livsci.2008.01.002

10.1194/jlr.M500362-JLR200

10.1016/j.lfs.2009.01.004

Suryawan, 2010, SNAT2 and LAT1 transporter abundance is developmentally regulated in skeletal muscle of neonatal pigs, FASEB J, 24, 331.4, 10.1096/fasebj.24.1_supplement.331.4

10.1194/jlr.M600396-JLR200

10.1371/journal.pone.0045087

10.1079/BJN20031079

10.1016/S0753-3322(02)00253-6

10.1007/s00726-011-0983-2

Duan, 2015, Nutritional and regulatory roles of leucine in muscle growth and fat reduction, Front Biosci (Landmark Ed), 20, 796, 10.2741/4338

10.1371/journal.pone.0029391

10.3920/978-90-8686-579-6

10.1152/japplphysiol.00428.2010

10.1007/s11745-004-1259-6

10.1152/physiol.00014.2006

10.1016/0005-2760(92)90166-S

10.1016/j.jnutbio.2008.05.014

10.1016/S0309-1740(99)00144-8

10.1016/S0301-6226(99)00129-3

10.2337/db10-1364

10.1194/jlr.M800535-JLR200

10.1097/00041433-200206000-00006

10.1017/S0007114513002584

10.1016/j.meatsci.2004.12.001

Larqué, 2006, Docosahexaenoic acid supply in pregnancy affects placental expression of fatty acid transport proteins, Am J Clin Nutr, 84, 853, 10.1093/ajcn/84.4.853

10.1194/jlr.M800093-JLR200

10.1074/jbc.M409598200

10.3181/0711-MR-311

Tan, 2008, Dietary l-arginine supplementation increases muscle gain and reduces body fat mass in growing-finishing pigs, Amino Acids, 27, 169

10.1002/jsfa.4237

10.1371/journal.pone.0036616

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British Journal of Nutrition

Tập 113 Số 5

739-748

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