Effects of dietary antioxidants on DNA damage in lysed cells using a modified comet assay procedure

Y.T Szeto1, A.R Collins2, I.F.F Benzie1
1Department of Nursing and Health Sciences, The Hong Kong Polytechnic University, Kowloon, Hong Kong, SAR, China
2DNA Instability Laboratory, The Rowett Research Institute, Aberdeen, Scotland, UK

Tài liệu tham khảo

Panayiotidis, 1997, Ex vivo assessment of lymphocyte antioxidant status using comet assay, Free Rad. Res., 27, 533, 10.3109/10715769709065793 Duthie, 1997, Quercetin and myricetin protect against hydrogen peroxide-induced DNA damage (strand breaks and oxidised pyrimidines) in human lymphocytes, Mutat. Res., 393, 223, 10.1016/S1383-5718(97)00107-1 Y.T. Szeto, I.F.F. Benzie, Effects of dietary antioxidants on human DNA ex vivo, Free Rad. Res., in press. Singh, 1988, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res., 175, 184, 10.1016/0014-4827(88)90265-0 Fairbairn, 1995, The comet assay: a comprehensive review, Mutat. Res., 339, 37, 10.1016/0165-1110(94)00013-3 Rojas, 1999, Single cell gel electrophoresis assay: methodology and applications, J. Chromatogr. B, 722, 225, 10.1016/S0378-4347(98)00313-2 Collins, 1997, The comet assay: what can it really tell us?, Mutat. Res., 375, 183, 10.1016/S0027-5107(97)00013-4 Kassie, 2000, Single cell gel electrophoresis assay: a new technique for human biomonitoring studies, Mutat. Res., 463, 13, 10.1016/S1383-5742(00)00041-7 Kasamatsu, 1996, Comparison of chemically induced DNA breakage in cellular and subcellular systems using the comet assay, Mutat. Res., 369, 1, 10.1016/S0165-1218(96)90041-5 Collins, 1997, Comet assay in human biomonitoring studies: reliability, validation, and applications, Environ. Mol. Mutagen., 30, 139, 10.1002/(SICI)1098-2280(1997)30:2<139::AID-EM6>3.0.CO;2-I Collins, 1996, Oxidative damage to DNA: do we have a reliable biomarker?, Environ. Health Perspect., 104, 465, 10.1289/ehp.96104s3465 Middleton, 2000, The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer, Pharmacol. Rev., 52, 673 Gey, 1998, Vitamins E plus C and interacting conutrients required for optimal health. A critical and constructive review of epidemiology and supplementation data regarding cardiovascular disease and cancer, Biofactors, 7, 113, 10.1002/biof.5520070115 McDermott, 2000, Antioxidant nutrients: current dietary recommendations and research update, J. Am. Pharm. Assoc., 40, 785, 10.1016/S1086-5802(16)31126-3 I.F.F. Benzie, Antioxidants: observational epidemiology, in: M.J. Sadler, J.J. Strain, B. Cabellero (Eds.), The Encyclopedia of Human Nutrition, Academic Press, London, 1998, pp. 106–115. Ames, 1993, Oxidants, antioxidants, and the degenerative diseases of aging, Proc. Natl. Acad. Sci. U.S.A., 90, 7915, 10.1073/pnas.90.17.7915 Collins, 1999, Oxidative DNA damage, antioxidants, and cancer, Bioassays, 21, 238, 10.1002/(SICI)1521-1878(199903)21:3<238::AID-BIES8>3.0.CO;2-3 Thomas, 1995, The role of free radicals and antioxidants: how do we know that they are working?, Crit. Rev. Food Sci. Nutr., 35, 21, 10.1080/10408399509527683 Yu, 1994, Cellular defenses against damage from reactive oxygen species, Physiol. Rev., 74, 139, 10.1152/physrev.1994.74.1.139 Sweetman, 1997, Effect of antioxidant vitamin supplementation on DNA damage and repair in human lymphoblastoid cells, Nutr. Cancer, 27, 122, 10.1080/01635589709514513 Noroozi, 1998, Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes, Am. J. Clin. Nutr., 67, 1210, 10.1093/ajcn/67.6.1210 Duthie, 1996, Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes, Cancer Res., 56, 1291 Green, 1994, Effect of diet and vitamin C on DNA strand breakage in freshly-isolated human white blood cells, Mutat. Res., 316, 91, 10.1016/0921-8734(94)90011-6 Singh, 1990, DNA damage and repair with age in individual human lymphocytes, Mutat. Res., 237, 123, 10.1016/0921-8734(90)90018-M Laughton, 1989, Antioxidant and pro-oxidant actions of the plant phenolics quercetin, gossypol and myricetin, effect on lipid peroxidation, hydroxyl radical generation and bleomycin-dependent damage to DNA, Biochem. Pharmacol., 38, 2859, 10.1016/0006-2952(89)90442-5 Rueff, 1992, Oxygen species and the genotoxicity of quercetin, Mutat. Res., 265, 75, 10.1016/0027-5107(92)90040-9 Zhizhina, 1997, Effect of metal ions and xenobiotics on endogenous oxidation of DNA, Biochemistry (Moscow), 62, 88 Inoue, 1992, Caffeic acid causes metal-dependent damage to cellular and isolated DNA through H2O2 formation, Carcinogenesis, 13, 1497, 10.1093/carcin/13.9.1497 Long, 2000, Artifacts in cell culture: rapid generation of hydrogen peroxide on addition of (−)epigallocatachin, (−)epigallocatechin gallate, (+)catechin, and quercetin to commonly used cell culture media, Biochem. Biophys. Res. Commun., 273, 50, 10.1006/bbrc.2000.2895