Effects of chronic ammonia nitrogen stress on hydrolases and interleukin 17–3 (IL-17–3) in clam Cyclina sinensis
Tóm tắt
Ammonia nitrogen is an unavoidable factor in aquaculture, and it may have a severe effect on clam breeding. To explore the toxic effect of ammonia nitrogen on the clam Cyclina sinensis, the clam was exposed to ammonia nitrogen (8.07 mg/L) for 768 h (32 days) and then challenged with Vibrio parahaemolyticus. During the exposure, the hydrolases (acid phosphatase, ACP; alkaline phosphatase, AKP; lysozyme, LZM) and the interleukin 17–3 (IL-17–3) gene were assessed. The result showed that during ammonia nitrogen exposure, the activity of ACP in the hepatopancreas showed an overall upward trend, and the activity of AKP and LZM showed a trend of increasing first and then decreasing. Following infection with V. parahaemolyticus, all the hydrolases activities fluctuated in 144 h. The full-length IL17-3 cDNA is 1027 bp in length and contains a 144-bp 5′-untranslated region (UTR), a 284-bp 3′-UTR with a poly (A) sequence, and an open reading frame (ORF) of 603 bp encoding an IL17-3 protein of 200 amino acids with a deduced molecular weight of 21,986.7 Da and a theoretical isoelectric point of 6.57. The expression of IL-17–3 m RNA was detected in all selected tissues of the clam. At the onset of ammonia nitrogen stress, the expression of IL-17–3 was significantly upregulated in the hepatopancreas of C. sinensis, and reached the maximum value at 6 h. Following infection with V. parahaemolyticus, the expression of IL-17–3 in the hepatopancreas was also significantly upregulated, and reached the maximum value at 48 h. Our study may provide the first evidence of the role of the main hydrolases and IL-17–3 in clam responding to ammonia nitrogen stress.
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