Effects of chronic Porphyromonas gingivalis lipopolysaccharide infusion on skeletal muscles in mice

The Journal of Physiological Sciences - Tập 69 - Trang 503-511 - 2019
Naoya Kawamura1,2, Yoshiki Ohnuki1, Ichiro Matsuo1,2, Kenji Suita1, Misao Ishikawa3, Yasumasa Mototani1, Kouichi Shiozawa1, Aiko Ito1,4, Yuka Yagisawa1,4, Yoshio Hayakawa1,5, Megumi Nariyama6, Daisuke Umeki4, Yuko Ujiie2, Kazuhiro Gomi2, Satoshi Okumura1
1Department of Physiology, Tsurumi University School of Dental Medicine, Yokohama, Japan
2Department of Periodontology, Tsurumi University School of Dental Medicine, Yokohama, Japan
3Department of Oral Anatomy, Tsurumi University School of Dental Medicine, Yokohama, Japan
4Department of Orthodontics, Tsurumi University School of Dental Medicine, Yokohama, Japan
5Department of Dental Anesthesiology, Tsurumi University School of Dental Medicine, Yokohama, Japan
6Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine, Yokohama, Japan

Tóm tắt

Periodontitis, which is caused by various oral organisms, predominantly affects adults, and is one of the main causes of tooth loss, as well as leading to progression of numerous systemic diseases. However, its relationship to sarcopenia (aging-associated degenerative loss of skeletal muscle mass and function) remains unclear. The aim of this study was to investigate the effects of Porphyromonas gingivalis lipopolysaccharide (PG-LPS) on skeletal muscle in mice, and to establish the underlying mechanisms. Mice (C57BL/6) were injected with PG-LPS (0.8 mg/kg/day) for 4 weeks. This treatment significantly decreased the weight of fast-twitch skeletal muscles (masseter and tibialis anterior muscles), but not that of slow-twitch skeletal muscle (soleus muscle). The area of fibrosis was significantly increased in masseter muscle, but remained unchanged in the other two muscles. The number of apoptotic myocytes was significantly increased (approximately eightfold) in masseter muscle. These data suggest that persistent subclinical exposure to PG-LPS might reduce the size of fast-twitch skeletal muscle, but not slow-twitch skeletal muscle. Masseter muscle appears to be especially susceptible to the adverse effects of PG-LPS, because muscle remodeling (muscle fibrosis and myocyte apoptosis) was induced solely in masseter muscle. Thus, periodontitis might be one of the major causes of oral sarcopenia.

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