Effects of abscisic acid (ABA) and ABA analogs on freezing tolerance, low-temperature growth, and flowering in rapeseed
Tóm tắt
Brassica napus and B. campestris are grown in Western Canada in areas subject to unseasonable frosts. At the seedling stage, cultivars of Brassica are very sensitive to frosts of -2° to-5°C, which are either lethal or delay the development of the plant. Seedlings of B. napus and B. campestris, germinated and grown at 10°C (16-h photoperiod), were treated with a foliar spray of either 100 μM racemic abscisic acid (ABA), 100 μM of various ABA analogs, 0.1% acetone, or were untreated. Freeze tests indicated 2°C of frost tolerance could be gained in B. napus following an application of three ABA analogs. In B. campestris, three analogs also increased freezing tolerance approximately 1.5°C. The analogs 2′,3′ dihydro ABA and acetylenic divinyl methyl-ABA were effective in both species. Plant fresh weight and dry weight increased in treated plants relative to control or acetone-treated plants after 3 weeks at 10°C. The effect of frost and/or analog treatment on flowering was determined in both species. In B. campestris and B. napus, a mild frost advanced flowering by approximately 2 days compared with nonfrozen control plants. The promotive effect of frost on flowering decreased with increasing severity of the frost. Several of the analog treatments, particularly 2′,3′ dihydro ABA and acetylenic divinyl ABA, advanced flowering by 2–3 days in both species. The benefit of these ABA analog treatments on flowering was enhanced additionally by a mild frost. Plants treated with either ABA, 2′,3′ dihydro ABA, 2′,3′ acetylenic dihydro ABA, or acetylenic divinyl ABA flowered up to 5 days earlier than control plants.
Tài liệu tham khảo
Abrams SR, Gusta LV (1989) Abscisic acid-related plant growth regulators—Germination promoters. European Patent No. 89403331.5
Biddington NL, Dearman AS (1982) The effect of abscisic acid on root and shoot growth of cauliflower plants. Plant Growth Regul 1:15–24
Blake TJ, Tan W, Abrams SR (1990) Antitranspirant action of abscisic acid and ten synthetic analogs in black spruce. Physiol Plant 80:365–370
Bray EA (1988) Drought- and ABA-induced changes in polypeptide and mRNA accumulation in tomato leaves. Plant Physiol 88:1210–1214
Chen HH, Li PH, Brenner MI (1983) Involvement of abscisic acid in potato cold acclimation. Plant Physiol 71:362–365
Chen THH, Gusta LV (1983) Abscisic acid-induced freezing resistance in cultured plant cells. Plant Physiol 73:71–75
Churchill GC, Ewan B, Reaney MJT, Abrams SR, Gusta LV (1992) Structure-activity relationships of abscisic acid analogs based on the induction of freezing tolerance in bromegrass (Bromus inermis Leyss) cell cultures. Plant Physiol 100:2024–2029
Close TJ, Kortt AA, Chandler PM (1989) A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Mol Biol 13:95–108
Creelman RA, Mason HS, Bensen RJ, Boyer JS, Mullet JE (1989) Water deficit and abscisic acid cause differential inhibition of shoot versus root growth in soybean seedlings: Analysis of growth, sugar accumulation, and gene expression. Plant Physiol 92:205–214
Cummins WR, Sondheimer E (1973) Activity of asymmetric isomers of abscisic acid in a rapid bioassay. Planta 111:365–369
Daie J, Campbell WF, Seeley SD (1981) Temperature-stress-induced production of abscisic acid and dihydrophaseic acid in warm and cool season crops. J Am Soc Hort Sci 106:11–13
Dörffling K, Schulenburg S, Lesselich G, Dörffling H (1990) Abscisic acid and proline levels in cold hardened winter wheat leaves in relation to variety-specific differences in freezing resistance. J Agr Crop Sci 165:230–239
Flores A, Grau A, Laurich F, Dörffling K (1988) Effect of new terpenoid analogues of abscisic acid on chilling and freezing resistance. J Plant Physiol 132:362–369
Fontes MR, Ozbun JL, Sadik S (1967) Influence of temperature on initiation of floral primordia in green sprouting broccoli. Proc Am Soc Hort Sci 91:315–320
Fowler DB, Limin AE, Robertson AJ, Gusta LV (1993) Breeding for low temperature tolerance in field crops. In: Buxton DR, et al. (eds) International crop science, vol 1. Crop Science Society of America, Madison, pp 357–362
Gusta LV, Ewan B, Reaney MJT, Abrams SR (1992) The effect of abscisic acid and abscisic acid metabolites on germination of cress seed. Can J Bot 70:1530–1535
Gusta L, Reaney MJT, Abrams SR, Robertson AJ, Abrams GD (1990) Abscisic acid analogs: Biological activity and interactions with the growth regulator abscisic acid. Com Agricul Food Chem 2:143–169
Heino P, Sandman G, Lang V, Nordin K, Palva ET (1990) Abscisic-acid deficiency prevents development of freezing tolerance in Arabidopsis thaliana (L.) Heynh. Theor Appl Genet 79:801–806
Houde M, Danyluk J, Laliberté JF, Rassart E, Dhindsa R, Sarhan F (1992) Cloning, characterization and expression of a cDNA encoding a 50 kD protein specifically induced by cold acclimation. Plant Physiol 99:1381–1387
Johnson-Flanagan AM, Huiwen Z, Thiagarajah MR, Saini HS (1991) Role of abscisic acid in the induction of freezing tolerance in Brassica napus suspension-cultured cells. Plant Physiol 95:1044–1048
Kurkela S, Franck M (1990) Cloning and characterization of a cold and ABA-inducible Arabidopsis gene. Plant Mol Biol 15:137–144
Lamb N, Abrams SR (1990) Synthesis of optically active cyclohexanone analogues of the plant hormone, abscisic acid. Can J Chem 68:1151–1162
Lamb N, Shaw AC, Abrams SR, Reaney MJT, Ewan B, Robertson AJ, Gusta LV (1993) Oxidation of the 8′-position of a biologically active abscisic acid analog. Phytochemistry 34:905–917
Lang V, Heino P, Palva ET (1989) Low temperature acclimation and treatment with exogenous abscisic acid induce common polypeptides in Arabidopsis thaliana (L.) Heynh. Theor Appl Genet 77:729–734
Lei B, Abrams SR, Ewan B, Gusta LV (1994) Non-chiral cyclohexadienone analogs of abscisic acid: Synthesis and biological activity. Phytochemistry (in press)
Mayer HJ, Rigassi N, Schwieter U, Weedon BCL (1976) Synthesis of abscisic acid. Helv Chim Acta 59:1424–1427
Miller CH, Konsler TR, Lamont WJ (1985) Cold stress influence on premature flowering of broccoli. Hort Sci 20:193–195
Oritani T, Yamashita K (1982) Synthesis and biological activity of (±)-2′,3′-dihydroabscisic acid. Agric Biol Chem 46:817–818
Orr W, Iu B, White TC, Robert LS, Singh J (1992) Complimentary DNA sequence of low temperature-induced Brassica napus gene with a homology to the Arabidopsis thaliana kin1 gene. Plant Physiol 98:1532–1534
Orr W, Keller WA, Singh J (1986) Induction of freezing tolerance in an embryogenic cell suspension culture of Brassica napus by abscisic acid at room temperature. J Plant Physiol 126:23–32
Ouellet F, Houde M, Sarhan F (1993) Purification, characteriza-tion and cDNA cloning of the 200 kDa protein induced by cold acclimation in wheat. Plant Cell Physiol 34:59–65
Rehm MM, Cline MG (1973) Rapid growth inhibition of Avena segments by abscisic acid. Plant Physiol 85:8–9
Skriver K, Mundy J (1990) Gene expression in response to abscisic acid and osmotic stress. Plant Cell 2:502–512
Suttle JC, Abrams SR (1993) Abscission-promoting activities of abscisic acid and five abscisic acid analogs in cotton seedlings and explants. Plant Growth Regul 12:111–117
Uknes SJ, Ho T-HD (1984) Mode of action of abscisic acid in barely aleurone layers: Abscisic acid induces its own conversion to phaseic acid. Plant Physiol 75:1126–1132
Walker-Simmons MK, Anderberg RJ, Rose PA, Abrams SR (1992) Optically pure ABA analogs: Tools for relating germination inhibition and gene expression in wheat embryos. Plant Physiol 99:501–507
Watts S, Rodriguez JL, Evans SE, Davies WJ (1981) Root and shoot growth of plants treated with abscisic acid. Ann Bot 47:595–602
Wilen RW, Hays DB, Mandel RM, Abrams SR, Moloney MM (1993) Competitive inhibition of abscisic acid-regulated gene expression by stereoisomeric acetylenic analogs of abscisic acid. Plant Physiol 101:469–476
Zeevaart JAD, Creelman RA (1988) Metabolism and physiology of abscisic acid. Ann Rev Plant Physiol Plant Mol Biol 39:439–473