Effects of a low-level semiconductor gallium arsenide laser on local pathological alterations induced by Bothrops moojeni snake venom

Photochemical & Photobiological Sciences - Tập 12 - Trang 1895-1902 - 2013
Elziliam Aranha de Sousa1, José Adolfo Homobono Machado Bittencourt1, Nayana Keyla Seabra de Oliveira1, Shayanne Vanessa Correia Henriques1, Leide Caroline dos Santos Picanço1, Camila Pena Lobato1, José Renato Ribeiro2, Washington Luiz Assunção Pereira3, José Carlos Tavares Carvalho4, Jocivânia Oliveira da Silva1
1Toxicology Laboratory, Pharmaceutical Science Course, Macapa, Brazil
2Zoonosis Service, Secretary of Health, Macapa, Brazil
3Animal Pathology Laboratory, Federal Rural University of Amazonia, Belém, Brazil
4Drugs Laboratory, Pharmaceutical Science Course, Macapa, Brazil

Tóm tắt

Antivenom therapy has been ineffective in neutralizing the tissue damage caused by snakebites. Among therapeutic strategies to minimize effects after envenoming, it was hypothesized that a low level laser would reduce complications and reduce the severity of local snake venom effects. In the current study, the effect of a low-level semiconductor gallium arsenide (GaAs) laser on the local pathological alterations induced by B. moojeni snake venom was investigated. The experimental groups consisted of five male mice, each administered either B. moojeni venom (VB), B. moojeni venom + antivenom (VAV), B. moojeni venom + laser (VL), B. moojeni venom + antivenom + laser (VAVL), or sterile saline solution (SSS) alone. Paw oedema was induced by intradermal administration of 0.05 mg kg−1 of B. moojeni venom and was expressed in mm of directly induced oedema. Mice received by subcutaneous route 0.20 mg kg−1 of venom for evaluating nociceptive activity and the time (in seconds) spent in licking and biting the injected paw was taken as an indicator of pain response. Inflammatory infiltration was determined by counting the number of leukocytes present in the gastrocnemius muscle after venom injection (0.10 mg kg−1). For histological examination of myonecrosis, venom (0.10 mg kg−1) was administered intramuscularly. The site of venom injection was irradiated by the GaAs laser and some animals received antivenom intraperitoneally. The results indicated that GaAs laser irradiation can help in reducing some local effects produced by the B. moojeni venom in mice, stimulating phagocytosis, proliferation of myoblasts and the regeneration of muscle fibers.

Tài liệu tham khảo

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