Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses

Ze Wu1, Hong Zheng1, Jian Gu1, Feng Li1, Rui long Lv1, Ya yun Deng1, Wan Xu1, Yong Tong1
1Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, China

Tóm tắt

Từ khóa


Tài liệu tham khảo

LIN YH, Cai L, Cheng ZS, et al. A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-nCoV) infected pneumonia (standard version). Military Med Res, 2020,7(1):.

She J, Jiang J, Ye L, et al. 2019 novel coronavirus of pneumonia in Wuhan, China: emerging attack and management strategies. Clin Transl Med, 2020,9(1):19

Ryu S, Chun BC. Korean Society of Epidemiology 2019-nCoV Task Force Team. An interim review of the epidemiological characteristics of 2019 novel coronavirus. Epidemiol Health, 2020,42:e2020006

Holshue ML, DeBolt C, Lindquist S, et al. First Case of 2019 Novel Coronavirus in the United States. N Engl J Med, 2020,382(10):929–936

Chinese National Health Committee. The latest epidemic situation of the new coronavirus pneumonia at 24:00 on Apirl 10 (Chinese). http://www.nhc.gov.cn/xcs/yqtb/202004/be5fdf84937844bcb88841c2c6bb8145.shtml.

Corman VM, Landt O, Kaiser M, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill, 2020,25(3):1–8

General Office of the Chinese National Health Commission. New coronavirus pneumonia diagnosis and treatment, a clinical guideline and database (trial version 6) (Chinese). 2020,02(01):e2–e2. doi: https://doi.org/10.3760/cma.j.issn.9999-998X.2020.01.002

Mahase E. Coronavirus: covid-19 has killed more people than SARS and MERS combined, despite lower case fatality rate. BMJ, 2020,368:m641

Binnicker MJ. Emergence of a Novel Coronavirus Disease (COVID-19) and the Importance of Diagnostic Testing: Why Partnership between Clinical Laboratories, Public Health Agencies, and Industry Is Essential to Control the Outbreak. Clin Chem, 2020,66(5):664–666

Kariwa H, Fujii AN, Takashima BI. Inactivation of SARS Coronavirus by Means of Povidone-Iodine, Physical Conditions and Chemical Reagents. Dermatology, 2006, 212 (Suppl 1):119–123