Effect of thidiazuron and gibberellic acid on leaf yellowing of cut stock flowers

Central European Journal of Biology - Tập 4 - Trang 461-468 - 2009
Antonio Ferrante1, Anna Mensuali-Sodi2, Giovanni Serra2
1Departament of Plant Production, Università degli Studi di Milano, Milano, Italy
2Sant’Anna School of Advanced Studies, Pisa, Italy

Tóm tắt

Plant hormones such as cytokinins and gibberellins are able to inhibit leaf yellowing in different species of cut flowers and potted plants. These hormones can be used alone or in combination among them for preserving chlorophyll in floriculture items. In the present study thidiazuron was tested alone or combined with GA3 for delaying leaf yellowing of cut stock flowers during vase life. Cut flowers were placed in a controlled environment and treated for 24 hours with the following solutions: distilled water (control) or solutions containing 5, 10 µM thidiazuron (TDZ), 0.5 mM gibberellic acid (GA3), or a combination of 0.5 mM GA3 with 5 µM TDZ. The effect of treatments was evaluated by measuring chlorophyll content, ethylene production, leaf gas exchanges and chlorophyll a fluorescence. Results showed that TDZ was able to delay leaf yellowing in light during whole experimental period (30 days). The effect of TDZ on dark stored flowers was less effective, and also delayed chlorophyll losses for 10–12 days. TDZ and GA3 combination did not show any synergistic nor beneficial effect. Gas exchange values such as net photosynthesis, vapour pressure deficit, stomatal conductance and water use efficiency were higher in the TDZ only treatment.

Tài liệu tham khảo

Buchanan-Wollaston V., Earl S., Harrison E., Mathas E., Navabpour S., Page T., et al., The molecular analysis of leaf senescence - a genomics approach, Plant Biotechnol. J., 2003, 1, 3–22 Ferrante A., Hunter D.A., Wesley P.H., Reid M.S., Thidiazuron - a potent inhibitor of leaf senescence in Alstroemeria, Postharvest Biol. Techn., 2002, 25, 333–338 Celikel F.G., Reid M.S., Postharvest handling of stock (Matthiola incana), HortSci., 2002, 37, 144–147 Jordi W., Stoopen G.M., Kelepouris K., Van Der Krieken W.M., Gibberellin-induced delay of leaf senescence of Alstroemeria cut flowering stems is not caused by an increase in the endogenous cytokinin content, J. Plant Growth Regul., 1995, 14, 121–127 Gan S.S., Amasino R.M., Cytokinins in plant senescence: from spray and pray to clone and play, Bioassays, 1996, 18, 557–565 Philosoph-Hadas S., Michaeli R., Reuveni Y., Meir S., Benzyladenine pulsing retards leaf yellowing and improves quality of goldenrod (Solidago canadensis) cut flowers, Postharvest Biol. Technol., 1996, 9, 65–73 Van Doorn W.G., Hibma J., De-Wit J., Effect of exogenous hormones on leaf yellowing in cut flowering branches of Alstroemeria pelegrina L., Plant Growth Regul., 1992, 11, 445–448 Genkov T., Ivanova I., Effect of cytokinin-active phenylurea derivatives on shoot multiplication, perioxidase and superoxide dismutase activities of in vitro cultured carnation, Bulg. J. Plant Physiol., 1995, 21, 73–83 Lichtenthaler H.K., Chlorophylls and carotenoids: pigments of photosynthetic membranes, Methods Enzymol., 1987, 148, 350–382 von Caemmerer S., Farquhar G.D., Some relationships between the biochemistry of photosynthesis and the gas-exchange of leaves, Planta, 1981, 153, 376–387 Francini A., Nali C., Picchi V., Lorenzini G., Metabolic changes in white clover clones exposed to ozone, Env. Exp. Bot., 2007, 60, 11–19 Ferrante A., Vernieri P., Serra G., Tognoni F., Changes in abscisic acid during leaf yellowing of cut stock flowers, Plant Growth Regul., 2004, 43, 127–134 Mutui T.M., Mibus H., Serek M., Effects of thidiazuron, ethylene, abscisic acid and dark storage on leaf yellowing and rooting of Pelargonium cuttings, J. Hort. Science Biotechnol., 2005, 80, 543–550 Ferrante A., Mensuali-Sodi A., Serra G., Tognoni F., Treatment with thidiazuron for preventing leaf yellowing in cut tulips, and chrysanthemum, Acta Hortic., 2003, 624, 357–363 Capelle S.C., Mok D.W.S., Kirchner S.C., Mok M.C., Effects of thidiazuron on cytokinin autonomy and the metabolism of N6-(Δ2-isopentenyl)[8-14C] adenosine in callus tissues of Phaseolus lunatus L, Plant Physiol., 1983, 73, 796–802 Kraepiel Y., Miginiac E., Photomorphogenesis and phytohormones, Plant Cell Env., 1997, 20, 807–812 Flores S., Tobin E.M., Benzyladenine modulation of the expression of two genes for nuclear encoded chloroplast proteins in Lemna gibba: apparent posttranscriptional regulation, Planta, 1986, 168, 340–349 Banowetz G.M., Cultivars of hexaploid wheat of contrasting stature and chlorophyll retention differ in cytokinin content and responsiveness, Ann. Bot., 1997, 79, 185–190 Whitman C.M., Heins R.D., Moe R., Funnell K.A., GA(4+7) plus benzyladenine reduce foliar chlorosis of Lilium longiflorum, Sci Hortic., 2001, 89, 143–154 Suttle J.C., Involvement of ethylene in the action of the cotton defoliant thidiazuron, Plant Physiol., 1985, 78, 272–276 Yip W.K., Yang S.F., Effect of thidiazuron, a cytokininactive urea derivative, in cytokinin-dependent ethylene production systems, Plant Physiol., 1986, 80, 515–519 Suttle J.C., Disruption of the polar auxin transport system in cotton seedlings following treatment with the defoliant thidiazuron, Plant Physiol., 1988, 86, 241–245 Chang H., Jones M.L., Banowetz G.M., Clark D.G., Overproduction of cytokinins in petunia flowers transformed with PSAG12-IPT delays corolla senescence and decreases sensitivity to ethylene, Plant Physiol., 2003, 132, 2174–2183 Mutui T.M., Mibus H., Serek M., Influence of thidiazuron, ethylene, abscisic acid and dark storage on the expression levels of ethylene receptors (ETR) and ACC synthase (ACS) genes in Pelargonium, Plant Growth Regul., 2007, 53, 87–96 Wachowicz M., Skutnik E., Rabiza-Świder J., The effect of growth regulators on stomatal aperture in senescing cut leaves of Zantedeschia aethiopica Spr. and Hosta Tratt. ‘Undulata Erromena’, Folia Horticulturae, 2006, 18, 65–75 Ferrante A., Maggiore T., Chlorophyll a fluorescence measurements to evaluate storage time and temperature of Valerianella leafy vegetables, Postharvest Biol. Technol., 2007, 45, 73–80 Pompodakis N.E., Terry L.A., Joyce D.C., Lydakis D.E., Papadimitriou M.D., Effect of seasonal variation and storage temperature on leaf chlorophyll fluorescence and vase life of cut roses, Postharvest Biol. Technol., 2005, 36, 1–8 Hossain A.B.M.S., Boyce A.N., Osman N., Postharvest quality, vase life and photosynthetic yield (chlorophyll fluorescence) of Bougainvillea flower by applying ethanol, Aust. J. Basic Appl. Sci., 2007, 1, 733–740