Effect of respiratory tract co-colonizers on initial attachment of Neisseria meningitidis
Tóm tắt
Respiratory tract is a complex system comprising of unique microbiota inhabitants. Neisseria meningitidis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa and Klebsiella pneumoniae are few prevalent bacteria in the community composition during lung infections. Although, N. meningitidis resides asymptomatically in nasopharynx of the human host, it can cause fatal infections like meningitis. However, factors affecting transit from carriage to symptomatic infection are not well understood. Various host metabolites and environmental conditions affect the virulence of bacteria. Here, we report that presence of co-colonizers significantly reduces the initial attachment of N. meningitidis to A549 nasopharyngeal epithelial cells. Further, significant decrease in invasion to A549 nasopharyngeal epithelial cells was observed. Moreover, survival in J774A.1 murine macrophage also increases significantly when conditioned media (CM) from S. pyogenes and L. rhamnosus is used for culturing N. meningitidis. The increase in survival could be attributed to increased capsule synthesis. The gene expression studies revealed increased expression of siaC and ctrB in CM prepared from the growth S. pyogenes and L. rhamnosus. Overall, the results suggest change in the virulence of N. meningitidis is assisted by lung microbiota.
Tài liệu tham khảo
Banerji R, Kanojiya P, Saroj SD (2020) Role of interspecies bacterial communication in the virulence of pathogenic bacteria. Crit Rev MicrobIol 46(2):136–146
Breakwell DP, Moyes RB, Reynolds J (2009) Differential staining of bacteria: capsule stain. Curr Protoc Microbiol 15(1):A3I1-A3I4
Chonmaitree T, Jennings K, Golovko G, Khanipov K, Pimenova M, Patel JA, McCormick DP, Loeffelholz FY (2017) Nasopharyngeal microbiota in infants and changes during viral upper respiratory tract infection and acute otitis media. PLoS One 12(7):1–15
Coffey BM, Anderson GG (2014) Biofilm formation in the 96-well microtiter plate. Methods Mol Biol (Clifton, N.J.) 1149:631–641
Curran CS, Bolig T, Torabi-Parizi P (2018) Mechanisms and targeted therapies for pseudomonas aeruginosa lung infection. AJRCCM 197(6):708–727
De Klerk N, Maudsdotter L, Gebreegziabher H, Saroj SD, Eriksson B, Eriksson OS, Roos S, Linden S, Sjolinder H, Jonsson AB (2016) Lactobacilli reduce Helicobacter pylori attachment to host gastric epithelial cells by inhibiting adhesion gene expression. Infect Immun 84(5):1526–1535
De Klerk N, Saroj SD, Wassing GM, Maudsdotter L, Jonsson AB (2017) The host cell transcription factor EGR1 is induced by bacteria through the EGFR–ERK1/2 pathway. Front Cell Infect 7:16
Engman J, Eriksson OS, Saroj SD, Zguna N, Lloris-Garcera P, Ilag LL, Jonsson AB (2017) Host cell-derived lactate functions as an effector molecule in Neisseria meningitidis microcolony dispersal. PLoS Pathog 13(4):1–27
Exley RM, Shaw J, Mowe E, Sun YH, West NP, Williamson M, Botto M, Smith H, Tang CM (2005) Available carbon source influences the resistance of Neisseria meningitidis against complement. JEM 201(10):1637–1645
Fang L, Zhou L, Tamm M, Roth M (2021) OM-85 Broncho-Vaxom®, a bacterial lysate, reduces SARS-CoV-2 binding proteins on human bronchial epithelial cells. Biomedicines 9(11):1544
Fomby P, Cherlin AJ (2011) Role of microbiota in immunity and inflammation. NIH 72(2):181–204
Guilhen C, Forestier C, Balestrino D (2017) Micro Review Biofilm dispersal : multiple elaborate strategies for dissemination of bacteria with unique properties. Mol Microbiol 105:188–210
Hathaway LJ, Bättig P, Reber S, Rotzetter JU, Aebi S, Hauser C, Heller M, Kadioglu A, Mühlemann K (2014) Streptococcus pneumoniae detects and responds to foreign bacterial peptide fragments in its environment. Open Biol 4(4):130224
Kanojiya P, Joshi R, Saroj SD (2022a) Availability of polyamines affects virulence and survival of Neisseria meningitidis. J Microbiol 60(6):640–648
Kanojiya P, Joshi R, Saroj SD (2022b) The source of carbon and nitrogen differentially affects the survival of Neisseria meningitidis in macrophages and epithelial cells. Arch Microbiol 204(7):1–9
Krasowska A, Sigler K (2014) How microorganisms use hydrophobicity and what does this mean for human needs? Front Cell Infect 4:1–7
Lux J, Holivololona L, San Millan Gutierrez R, Hilty M, Ramette A, Heller M, Hathaway LJ (2022) AmiA and AliA peptide ligands are secreted by Klebsiella pneumoniae and inhibit growth of Streptococcus pneumoniae. Sci Rep. 12(1):22268
Maekawa T, Kashkar H, Coll NS (2023) Dying in self-defence: a comparative overview of immunogenic cell death signalling in animals and plants. Cell Death Differ 30(2):258–268
Nasher F, Aguilar F, Aebi S, Hermans PW, Heller M, Hathaway LJ (2018) Peptide ligands of AmiA, AliA, and AliB proteins determine pneumococcal phenotype. Front Microbiol 9:3013
Pizza M, Rappuoli R (2015) Neisseria meningitidis: pathogenesis and immunity. Curr Opin Microbiol 23:68–72
Raudoniute J, Bironaite D, Bagdonas E, Kulvinskiene I (2023) Human airway and lung microbiome at the crossroad of health and disease (Review). Exp Ther Med 25(1):18
Roszak DB, Colwell RR (1987) Survival strategies of bacteria in the natural environment. Microbiol Rev 51(3):365–379
Saroj SD, Maudsdotter L, Tavares R, Jonsson AB (2016) Lactobacilli interfere with streptococcus pyogenes hemolytic activity and adherence to host epithelial cells. Front Microbiol 7:1–8
Schoen C, Kischkies L, Elias J, Ampattu BJ (2014) Metabolism and virulence in Neisseria meningitidis. Front Cell Infect 4:1–16
Sigurlásdóttir S, Saroj SD, Eriksson O, Jonsson AB (2018) Quantification of Neisseria meningitidis adherence to human epithelial cells by colony counting. Bio-Protoc 8(3):1–10
S. Sigurlásdóttir (2018) Influence of host and bacterial factors during Neisseria meningitidis colonization (Issue December)
Smakman F, Hall AR (2022) Exposure to lysed bacteria can promote or inhibit growth of neighboring live bacteria depending on local abiotic conditions. FEMS Microbiol Ecol 98(2):fiac011
Sorbara MT, Pamer EG (2019) Interbacterial mechanisms of colonization resistance and the strategies pathogens use to overcome them. Mucosal Immunol 12(1):1–9
Spinosa MR, Progida C, Talà A, Cogli L, Alifano P (2007) The Neisseria meningitidis capsule is important for intracellular survival in human cells. Infect Immun 75(7):3594–3603
Stephens DS, Greenwood B, Brandtzaeg P (2007) Epidemic meningitis, meningococcaemia, and Neisseria meningitidis. Lancet 369(9580):2196–2210
Swain CL, Martin DR, Sim D, Jordan TW, Mackichan JK (2017) Survival of Neisseria meningitidis outside of the host: environmental effects and differences among strains. Epidemiol Infect 145(16):3525–3534
Tunbridge AJ, Stevanin TM, Lee M, Marriott HM, Moir JW, Read RC, Dockrell DH (2006) Inhibition of macrophage apoptosis by Neisseria meningitidis requires nitric oxide detoxification mechanisms. Infect Immun 74(1):729–733
Tzeng YL, Thomas J, Stephens DS (2016) Regulation of capsule in Neisseria meningitidis. Crit Rev Microbiol 42(5):759–772
Ursell LK, Metcalf JL, Parfrey LW, Knight R (2012) Defining the human microbiome. Nutr Rev 299(23):1278–1280
Yagi K, Huffnagle GB, Lukacs NW, Asai N (2021) The lung microbiome during health and disease. Int J Mol Sci 22(19):1–13