Effect of high-fat diet on secreted milk transcriptome in midlactation mice

Physiological Genomics - Tập 49 Số 12 - Trang 747-762 - 2017
Yulu Chen1, Jun Wang2, Shumin Yang1, Sagar M. Utturkar3, Jennifer Crodian1, Shelby Cummings1, Jyothi Thimmapuram2, Phillip San Miguel4, Shihuan Kuang1, Michael Gribskov3, Karen Plaut1, Theresa Casey1
1Department of Animal Sciences, Purdue University, West Lafayette, Indiana
2Department of Biological Sciences, Purdue University, West Lafayette, Indiana
3Bioinformatics Core, Purdue University, West Lafayette, Indiana
4Genomics Core at Purdue University, West Lafayette, Indiana

Tóm tắt

High-fat diet (HFD) during lactation alters milk composition and is associated with development of metabolic diseases in the offspring. We hypothesized that HFD affects milk microRNA (miRNA) and mRNA content, which potentially impact offspring development. Our objective was to determine the effect of maternal HFD on secreted milk transcriptome. To meet this objective, 4 wk old female ICR mice were divided into two treatments: control diet containing 10% kcal fat and HFD containing 60% kcal fat. After 4 wk on CD or HFD, mice were bred while continuously fed the same diets. On postnatal day 2 (P2), litters were normalized to 10 pups, and half the pups in each litter were cross-fostered between treatments. Milk was collected from dams on P10 and P12. Total RNA was isolated from milk fat fraction of P10 samples and used for mRNA-Seq and small RNA-Seq. P12 milk was used to determine macronutrient composition. After 4 wk of prepregnancy feeding HFD mice weighed significantly more than did the control mice. Lactose and fat concentration were significantly ( P < 0.05) higher in milk of HFD dams. Pup weight was significantly greater ( P < 0.05) in groups suckled by HFD vs. control dams. There were 25 miRNA and over 1,500 mRNA differentially expressed (DE) in milk of HFD vs. control dams. DE mRNA and target genes of DE miRNA enriched categories that were primarily related to multicellular organismal development. Maternal HFD impacts mRNA and miRNA content of milk, if bioactive nucleic acids are absorbed by neonate differences may affect development.

Từ khóa


Tài liệu tham khảo

10.3305/nh.2014.30.2.7416

10.1111/j.1432-1033.1982.tb06660.x

10.4236/fns.2011.23027

10.3390/ijerph121113981

10.3390/ijms17060956

10.1038/srep20680

10.1186/bcr1653

10.1002/mnfr.201500222

10.12688/f1000research.8548.1

10.1113/jphysiol.2007.146316

10.1186/1471-2164-10-548

10.1016/j.nut.2008.03.020

10.1016/j.mce.2011.10.008

10.2527/jas.2012-5764

10.1371/journal.pone.0021305

10.1038/srep05297

10.3390/ijms17101712

10.1523/JNEUROSCI.2642-08.2008

10.1371/journal.pone.0034872

10.3402/jev.v3.23743

10.2174/157015906776359540

10.1186/s12917-016-0881-7

10.1111/1753-0407.12313

10.1186/s12986-017-0168-4

10.1093/jn/127.3.458

10.1093/jn/129.8.1574

10.1101/cshperspect.a001800

10.1007/s10911-009-9158-0

10.1016/j.siny.2016.04.012

10.1038/srep44605

10.1016/j.peptides.2015.05.003

10.1017/S000711450820749X

10.1038/nrg2290

10.1677/joe.0.1030213

10.1186/gb-2003-4-3-207

10.1371/journal.pone.0119784

10.1038/ncb1929

10.1371/journal.pone.0017261

10.7150/ijbs.7.567

10.1186/s12864-015-1896-3

10.3168/jds.2012-5489

10.1371/journal.pone.0049463

10.1186/1758-907X-1-7

10.1556/APhysiol.99.2012.2.8

10.1093/nar/gkt1181

10.1080/15476286.2015.1017212

10.1021/ac100556g

10.1186/s12864-015-1965-7

10.1371/journal.pone.0154129

10.1093/ajcn/77.4.931

10.4161/cbt.12.6.16300

10.1006/meth.2001.1262

10.1136/bmj.1.6119.1018

10.1016/j.jchromb.2008.11.003

10.1093/nar/gkp292

10.1002/bies.200800212

10.1186/s12986-016-0101-2

10.1016/j.celrep.2017.02.039

10.1186/1471-2164-15-1012

10.1371/journal.pone.0050564

10.1186/1746-4358-5-3

10.1139/apnm-2015-0702

10.1016/j.mcn.2016.12.004

10.1371/journal.pone.0152545

10.1016/j.physbeh.2011.05.012

10.1186/1471-2164-11-320

10.1007/s003359900338

10.2527/jas.2005-609

10.1161/HYPERTENSIONAHA.107.101477

10.1016/S0092-8674(00)80878-8

10.1096/fj.13-234823

10.1016/j.neuron.2014.03.016

10.3945/jn.113.178772

10.1016/j.als.2016.11.007

10.2337/db11-0957

10.1186/s12864-015-2044-9

10.1111/j.1460-9568.2010.07340.x

10.1002/jcb.22733

10.1074/jbc.M114.588046

10.1002/jcp.24304

10.1074/jbc.M115.676734

10.1111/acel.12373

10.1038/srep20032

10.3168/jds.2012-5748

10.1186/1471-2164-13-45

10.3945/jn.115.218586

10.1038/ejcn.2015.168

10.3945/an.112.002238

Zabielski R, 2008, J Physiol Pharmacol, 59, 35

10.1074/jbc.M111.330381