Effect of drought and low light on growth and enzymatic antioxidant system of Picea asperata seedlings
Tóm tắt
The combined effects of drought and low light on biomass partition, foliar nitrogen concentration, membrane stability and active oxygen species (AOS) and antioxidant system were investigated in dragon spruce (Picea asperata Mast.) seedlings grown at two watering regimes (well-watered, 100% of field capacity and drought, 30% of field capacity) and light availabilities (HL, 100% of full sunlight and low light, 15% of full sunlight). Under high light condition drought not only reduced foliar nitrogen concentration (Nmass) and membrane stability index (MSI) but also significantly increased biomass partitioning to roots, AOS, ascorbic acid (AsA) content and antioxidant enzyme activities including superoxide dismutase (SOD, EC 1.15.1.1), peroxidase (POD, EC 1.11.1.7), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase(GR, EC 1.6.4.2). However, no prominently drought-induced differences in biomass partitioning to root, SOD, GR activities, hydrogen peroxide (H2O2) and MSI were observed in low light seedlings. On the other hand, significant interaction of drought and low light was found on MSI, the antioxidant enzymes activities (SOD, POD, CAT, APX, GR), H2O2 and superoxide radical (O2
−). These results suggested that seedlings grown at the understory were more sensitive to drought than low light.
Tài liệu tham khảo
Alscher RG (1989) Biosynthesis and antioxidant properties of glutathione in plants. Physiol Plant 77:457–464
Aranda I, Castro L, Pardos M, Gil L, Pardos JA (2005) Effects of the interaction between drought and shade on water relations, gas exchange and morphological traits in cork oak (Quercus suber L.) seedlings. For Ecol Manag 210:117–129
Becana M, Tejo PA, Irigoyen JJ, Sánchez-Díaz M (1986) Some enzymes of hydrogen peroxide metabolism in leaves and root nodules of Medicago sativa. Plant physiol 82:1169–1171
Bowler C, Montagu MV, Inze D (1992) Superoxide dismutase and stress tolerance. Ann Rev Plant Physiol Plant Mol Boil 43:83–116
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Canham CD, Berkowitz AR, Kelly VR, Lovett GM, Ollinger SV, Schnurr J (1996) Biomass allocation and multiple resource limitation in tree seedlings. Can J For Res 26:1521–1530
Castillo FI, Penel I, Greppin H (1984) Peroxidase release induced by ozone in Sedum album leaves. Plant Physiol 74:846–851
Chaitanya KV, Sundar D, Masilamani S, Reddy AR (2002) Variation in heat stress-induced antioxidant enzyme activities among three mulberry cultivars. Plant Growth Regul 36:175–180
Climent JM, Aranda I, Alonso J, Pardos JA, Gil L (2006) Developmental constraints limit the response of Canary Island pine seedlings to combined shade and drought. For Ecol Manag 231:164–168
Evans JR (1989) Phototsynthesis and nitrogen relationships in leaves of C3 plants. Oecologia 78:9–19
Foyer CH (2001) Prospects for enhancement of the soluble antioxidants, ascorbate and glutathione. Biofactors 15:75–78
Foyer CH, Halliwell B (1976) The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolis. Planta 133:21–25
Geβler A, Jung K, Gasche R, Papen H, Heidenfelder A, Borner E, Metzler B, Augustin S, Hildebrand E, Rennenberg H (2005) Climate and forest management influence nitrogen balance of European beech forests: microbial N transformations and inorganic N transformations and inorganic N net uptake capacity of mycorrhizal roots. Eur J For Res 124:95–111
Guo Z, Ou W, Lu S, Zhong Q (2006) Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiol Biochem 44:828–836
Hodges DM, Andrew CJ, Johnson DA, Hamilton RI (1996) Antioxidant compound responses to chilling stress in differentially sensitive inbred maize lines. Physiol Plant 98:685–692
Jablonski PP, Anderson JW (1978) Light-dependent reduction of oxidized glutathione by ruptured chloroplasts. Plant Physiol 61:221–225
Jebara S, Jebara M, Limam F, Aouani ME (2005) Changes in ascorbate peroxidase, catalase, guaiacol peroxidaes and superoxide dismutase activities in common bean (Phaseolus vulgaris) nodules under salt stress. J Plant Physiol 162:929–936
Ke D, Wang A, Sun G, Dong L (2002) The effect of active oxygen on the activity of ACC synthase induced by exogenous IAA. Acta Bot Sin 44:551–556
Kováčik J, Repčák M, Kron I (2006) Nitrogen deficiency induced changes of free amino acids and coumarin contents in the leaves of Matricaria chamomilla. Acta Physiol Plant 28:159–164
Lei YB, Yin CY, Li CY (2006) Differences in some morphological, physiological, and biochemical responses to drought stress in two contrasting populations of Populus Przewalskii. Physiol Plant 127:182–191
Liu Q (2002) Ecological research on subalpine coniferous forests in China. Sichuan University Press, Chengdu, pp 224
Luck H (1974) Catalases. In: Bergmeyer HU (ed) Methods of enzymatic analyses, vol 2. Academic Press, New York
Marshall JD (1986) Drought and shade interact to cause fine-root mortality in Douglas-fir seedlings. Plant Soil 91:51–60
Nahm M, Matzarakis A, Rennenberg H, Geβler A (2007) Seasonal courses of key parameters of nitrogen, carbon and water balance in European beech (Fagus sylvatica L.) grown on four different study sites along a European North-South climate gradient during the 2003 drought. Trees 21:79–92
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
Nelson DW, Sommers LE (1975) A rapid and accurate procedure for estimation of organic carbon in soils. Proc Indiana Acad Sci (for 1974) 84:456–462
Noctor G, Jovanovic SV, Driscoll S, Novitskaya L, Foyer CH (2002) Drought and oxidative load in the leaves of C3 plants: a predominant role for photorespiration. Ann Bot 89:841–850
Prider JN, Facelli JM (2004) Interactive effects of drought and shade on three arid zone chenopod shrubs with contrasting distributions in relation to tree canopies. Funct Ecol 18:67–76
Prochazkova D, Sairam RK, Srivastava GC, Singh DV (2001) Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Sci 161:765–771
Ramachandra Reddy A, Chaitanya KV, Jutur PP, Sumithra K (2004) Differential antioxidative responses to water stress among five mulberry (Morus alba L.) cultivars. Environ Exper Bot 52:33–42
Reddy AR, Chaitanya KV, Jutur PP, Sumithra K (2004) Differential antioxidative responses to water stress among five mulberry (Morus alba L.) cultivars. Envrion Exp Bot 52:33–42
Rousset O, Lepart J (2000) Positive and negative interactions at different life stages of a colonizing species (Quercus humilis). J Ecol 88:401–412
Sairam RK, Saxena DC (2000) Oxidative stress and antioxidants in wheat genotypes: possible mechanism of water stress tolerance. J Agron Crop Sci 184:55–61
Shanahan JF, Edwards IB, Quick JS, Fenwick JR (1990) Membrane thermostability and heat tolerance of spring wheat. Crop Sci 30:247–251
Shao HB, Chu LY, Wu G, Zhang JH, Lu ZH, Hu YC (2007) Changes of some anti-oxidative physiological indices under soil water deficits among 10 wheat (Triticum aestivum L.) genotypes at tillering stage. Colloids Surf B Biointerfaces 54:143–149
Shao HB, Chu LY, Zhao CX, Guo QJ, Liu XA, Jean-Marcel R (2006) Plant gene regulatory network system under abiotic stress. Acta Biol Szeged 50(1/2):1–9
Shao HB, Liang ZS, Shao MA (2005a) Changes of anti-oxidative enzymes and MDA content under soil water deficits among 10 wheat (Triticum aestivum L.) genotypes at maturation stage. Colloids Surf B Biointerfaces 45:7–13
Shao HB, Liang ZS, Shao MA (2005b) Adaptation of higher plants to environmental stress and stress signal transduction. Acta Ecol Sin 25(7):1772–1781
Smith TM, Huston MA (1989) A theory of the spatial and temporal dynamics of plant communities. Vegetatio 83:49–69
Smirnoff N, Wheeler GL (2000) Ascorbic acid in plants: biosynthesis and function. Crit Rev Plant Rev 19:267–290
Türkan İ, Bor M, Özdemir F, Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci 168:223–231
Verhoeven AS, Swanberg A, Thao M, Whiteman J (2005) Seasonal changes in leaf antioxidant systems and xanthophylls cycle characteristics in Taxus × media growing in sun and shade environments. Physiol Plant 123:428–434
