Effect of differentiation inhibiting activity, leukemia inhibitory (DIA/LIF), and glial cell-line neurotrophic (GDNF) factors on boar spermatogenic cells in culture

Russian Agricultural Sciences - Tập 37 - Trang 333-336 - 2011
I. P. Savchenkova1, E. V. Viktorova1, L. K. Ernst1
1Kovalenko All-Russian Experimental Veterinary Research Institute, Moscow, Russia

Tóm tắt

Maintenance of the phenotype of spermatogenic stem cells in vitro depends on the microenvironment and a number of factors. Culturing boar type A1–A4 spermatogonia on the STO feeder layer and addition to the growth medium of a conditioned medium containing DIA/LIF led over the course of 3–4 weeks to the formation of colonies which had morphology similar to embryonic stem cells and were distinguished by a high alkaline phosphatase activity.

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