1Department of Chemical Engineering, University of Florida, Gainesville, Florida, 32611
Tóm tắt
AbstractThe implication of the possible existence of differences in the times required for plasmid‐bearing and non‐plasmid‐bearing microorganisms to adjust their metabolic activities to step changes in their environment is examined. This adaptability difference suggests the possibility of maintaining an engineered strain in continuous culture by transient operation. It is shown for the case where adaptability is neglected that no cycling strategy will prevent the washout of the engineered strain, but the addition to the model of a time delay in substrate utilization can result in coexistence upon cycling. Numerical simulations of cycling in feed substrate concentration are carried out to illustrate the concept Operating diagrams are also constructed to indicate the conditions under which washout of the plasmid bearing strain can be prevented.
Từ khóa
Tài liệu tham khảo
10.1111/j.1749-6632.1981.tb14172.x
10.1002/bit.260260113
10.1002/bit.260271014
10.1002/bit.260271207
10.1002/btpr.5420010111
10.1002/bit.260271211
10.1002/btpr.5420010407
10.1098/rstb.1982.0065
10.1002/bit.260260819
10.1002/bit.260290603
Bailey J. E., 1979, Biochemical Engineering Fundamentals
