Effect of chronic smoking on lipid peroxidation and antioxidant status in gastric carcinoma patients
Tóm tắt
Oxidative stress plays an important role in malignant transformation and is postulated to be associated with increased lipid peroxidation. We determined the effects of chronic cigarette smoking on lipid peroxidation and antioxidant status in 100 male patients with gastric cancer and an equal number of age-matched healthy control subjects. The mean (SD) level of thiobarbituric acid reactive substances (TBARS) was higher in plasma (healthy non-smokers 3.1 [0.2]; healthy smokers 4.6 [0.2]; gastric cancer non-smokers 6.5 [1.0]; gastric cancer smokers 8.9 [3.1]) and erythrocytes (3.3 [0.6]; 4.6 [0.1]; 8.3 [0.9]; 13.2 [5.1]) from gastric cancer patients when compared with control subjects. TBARS level was higher in smokers than non-smoking gastric cancer patients. The activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-treansferase, reduced glutathione, and vitamins A, E and C were decreased in gastric cancer patients who were smokers as compared to other groups (p<0.001). Thus, there occurs lipid peroxidation and possible breakdown of antioxidant status in cigarette smoking, which may increase the risk of gastric cancer.
Tài liệu tham khảo
Kim Y, Shin A, Gwack J, et al. Cigarette smoking and gastric cancer risk in a community-based cohort study in Korea. Prev Med Pub Health 2007;40:467–474.
Yagi K. Lipid peroxides and human diseases. Chem Phys Lipids 1978;45:337–351.
Donnan SK. The thiobarbituric acid test applied to tissues from rats treated in various ways. J Biol Chem 1950;182:415–419.
Kakkar PS, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130–132.
Sinha KA. Colorimetric assay of catalase. Anal Biochem 1972;47:389–394.
Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium: biochemical roles as a component of glutathione peroxidase. Science 1973;179:588–590.
Habig WH, Pabst MJ, Jakoby WB. Glutathione-S-transferases, the first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130–7139.
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70–77.
Beutler E, Kelley BM. The effect of sodium nitrate on red cell glutathione. Experientia 1963;19:96–97.
Arivazhagan S, Kavitha K, Nagini S. Erythrocyte lipid peroxidation and antioxidants in gastric cancer patients. Cell Biochem Funct 1997;15:15–18.
Hwang TS, Choi HK, Han HS. Differential expression of manganese superoxide dismutase, copper/zinc superoxide dismutase, and catalase in gastric adenocarcinoma and normal gastric mucosa. EJSO 2007;33:474–479.
Masella R, Di Benedetto R, Vari R, Filesi C, Giovannini C. Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes. J Nutr Biochem 2005;16:577–586.