Effect of Silver Nanoparticles on Phenolic Compounds Production and Biological Activities in Hairy Root Cultures of Cucumis Anguria
Tóm tắt
The present study describes the elicitor effect of silver ion (Ag+) and biologically synthesized silver nanoparticles (AgNPs) to enhance the biomass accumulation and phenolic compound production as well as biological activities (antioxidant, antimicrobial and anticancer) in genetically transformed root (hairy root) cultures of Cucumis anguria. The biomass of hairy root cultures was signifcantly increased by AgNPs whereas decreased in Ag+ elicitation at 1 and 2 mg/L. AgNPs-elicited hairy roots produced a signifcantly higher amount of individual phenolic compounds (favonols, hydroxycinnamic and hydroxybenzoic acids), total phenolic and favonoid contents than Ag+-elicited hairy roots. Moreover, antioxidant, antimicrobial and anticancer activities were signifcantly higher following AgNPs-elicitation compared with that in Ag+-elicited hairy roots. We suggest that AgNPs could be an effcient elicitor in hairy root cultures to increase the phytochemical production.
Tài liệu tham khảo
Chen, W. H., Xu, C. M., Zeng, J. L., Zhao, B., Wang, X. D., Wang, Y. C. (2007) Improvement of echinacoside and acteoside production by two-stage elicitation in cell suspension culture of Cistanche deserticola. World J. Microbiol. Biotechnol. 23, 1451–1458.
Chung, I. M., Rekha, K., Rajakumar, G., Thiruvengadam, M. (2017) Jasmonic and salicylic acids enhanced phytochemical production and biological activities in cell suspension cultures of spine gourd (Momordica dioica Roxb). Acta Biol. Hung. 68, 88–100.
Fazal, H., Abbasi, B. H., Ahmad, N., Ali, M. (2016) Elicitation of medicinally important antioxidant secondary metabolites with silver and gold nanoparticles in callus cultures of Prunella vulgaris L. Appl. Biochem. Biotechnol. 180, 1076–1092.
Ge, X., Wu, J. (2005) Tanshinone production and isoprenoid pathways in Salvia miltiorrhiza hairy roots induced by Ag+ and yeast elicitor. Plant Sci. 168, 487–491.
Ghanati, F., Bakhtiarian, S. (2013) Changes of natural compounds of Artemisia annua L. by methyl jasmonate and silver nanoparticles. Adv. Env. Biol. 7, 2251–2258.
Ghanati, F., Bakhtiarian, S., Parast, B. M., Behrooz, M. K. (2014) Production of new active phyto-compounds by Achillea millefolium L. after elicitation with silver nanoparticles and methyl jas-monate. Biosci. Biotechnol. Res. Asia 11, 391–399.
Ghasemi, B., Hosseini, R., Nayeri, F. D. (2015) Effects of cobalt nanoparticles on artemisinin production and gene expression in Artemisia annua. Turk. J. Bot. 39, 769–777.
Jamshidi, M., Ghanati, F., Rezaei, A., Bemani, E. (2016) Change of antioxidant enzymes activity of hazel (Corylus avellana L.) cells by AgNPs. Cytotechnology 68, 525–530.
Krishnaraj, C., Jagan, G., Ramachandran, R., Abirami, S. M., Mohan, N., Kalaichelvan, P. T. (2012) Effect of biologically synthesized silver nanoparticles on Bacopa monnieri L. Wettst. plant growth metabolism. Process Biochem. 47, 651–658.
Lee, W. M., Kwak, J. I., An, Y. J. (2012) Effect of silver nanoparticles in crop plants Phaseolus radiatus and Sorghum bicolor: media effect on phytotoxicity. Chemosphere 86, 491–499.
Li, B., Wang, B., Li, H., Peng, L., Ru, M., Liang, Z., Yan, X., Zhu, Y. (2016) Establishment of Salvia castanea Diels f. tomentosa Stib. hairy root cultures and the promotion of tanshinone accumulation and gene expression with Ag+, methyl jasmonate, and yeast extract elicitation. Protoplasma 253, 87–100.
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.
Qian, H., Peng, X., Han, X., Ren, J., Sun, L., Fu, Z. (2013) Comparison of the toxicity of silver nanoparticles and silver ions on the growth of terrestrial plant model Arabidopsis thaliana. J. Env. Sci. 25, 1947–1956.
Shakeran, Z., Keyhanfar, M., Asghari, G., Ghanadian, M. (2015) Improvement of atropine production by different biotic and abiotic elicitors in hairy root cultures of Datura metel. Turk. J. Biol. 39, 111–118.
Spinoso-Castillo, J. L., Chavez-Santoscoy, R. A., Bogdanchikova, N., Pérez-Sato, J. A., Morales-Ramos, V., Bello-Bello, J. J. (2017) Antimicrobial and hormetic effects of silver nanoparticles on in vitro regeneration of vanilla (Vanilla planifolia Jacks. ex Andrews) using a temporary immersion system. Plant Cell Tiss. Org. Cult. 129, 195–207.
Thiruvengadam, M., Chung, I. M. (2015) Selenium, putrescine, and cadmium infuence health-promoting phytochemicals and molecular-level effects on turnip (Brassica rapa ssp. rapa). Food Chem. 173, 185–193.
Thiruvengadam, M., Gurunathan, S., Chung, I. M. (2015) Physiological, metabolic, and transcrip-tional effects of biologically-synthesized silver nanoparticles in turnip (Brassica rapa ssp. rapa L.). Protoplasma 252, 1031–1046.
Weremczuk-Jezyna, I., Grzegorczyk-Karolak, I., Frydrych, B., Królicka, A., Wysokińska, H. (2013) Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential. Acta Physiol. Plant. 35, 2095–2103.
Xing, B., Yang, D., Guo, W., Liang, Z., Yan, X., Zhu, Y., Liu, Y. (2015) Ag+ as a more effective elicitor for production of tanshinones than phenolic acids in Salvia miltiorrhiza hairy roots. Molecules 20, 309–324.
Yan, Q., Shi, M., Ng, J., Wu, J. Y. (2006) Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Salvia miltiorrhiza hairy roots. Plant Sci. 170, 853–858.
Yoon, J. Y., Chung, I. M., Thiruvengadam, M. (2015) Evaluation of phenolic compounds, antioxidant and antimicrobial activities from transgenic hairy root cultures of gherkin (Cucumis anguria L.). S. Afr. J. Bot. 100, 80–86.
Zhang, B., Zheng, L. P., Li, Y. W., Wang, J. W. (2013) Stimulation of artemisinin production in Artemisia annua hairy roots by Ag-SiO2 core-shell nanoparticles. Curr. Nanosci. 9, 363–370.
Zhang, C. H., Yan, Q., Cheuk, W. K., Wu, J. Y. (2004) Enhancement of tanshinone production in Salvia miltiorrhiza hairy root culture by Ag+ elicitation and nutrient feeding. Planta Med. 70, 147–151.