Effect of Light Intensity and Quality on Growth Rate and Composition of Chlorella vulgaris

Plants - Tập 9 Số 1 - Trang 31
Maria N. Metsoviti1, George Papapolymerou2, Ioannis Τ. Karapanagiotidis3, Ν. Katsoulas1
1Laboratory of Agricultural Constructions and Environmental Control, Department of Agriculture Crop Production and Rural Environment, University of Thessaly, Fytokou Street, 38446, Volos, Greece
2Department of Environmental Sciences, University of Thessaly, 41110 Larissa, Greece
3Aquaculture Laboratory, Department of Ichthyology and Aquatic Environment, University of Thessaly, Fytokou Street, 38446, Volos, Greece

Tóm tắt

In this research, the effect of solar irradiance on Chlorella vulgaris cultivated in open bioreactors under greenhouse conditions was investigated, as well as of ratio of light intensity in the 420–520 nm range to light in the 580–680 nm range (I420–520/I580–680) and of artificial irradiation provided by red and white LED lamps in a closed flat plate laboratory bioreactor on the growth rate and composition. The increase in solar irradiance led to faster growth rates (μexp) of C. vulgaris under both environmental conditions studied in the greenhouse (in June up to 0.33 d−1 and in September up to 0.29 d−1) and higher lipid content in microalgal biomass (in June up to 25.6% and in September up to 24.7%). In the experiments conducted in the closed bioreactor, as the ratio I420–520/I580–680 increased, the specific growth rate and the biomass, protein and lipid productivities increased as well. Additionally, the increase in light intensity with red and white LED lamps resulted in faster growth rates (the μexp increased up to 0.36 d−1) and higher lipid content (up to 22.2%), while the protein, fiber, ash and moisture content remained relatively constant. Overall, the trend in biomass, lipid, and protein productivities as a function of light intensity was similar in the two systems (greenhouse and bioreactor).

Từ khóa


Tài liệu tham khảo

Mata, 2010, Microalgae for biodiesel production and other applications: A review, Renew. Sustain. Energy Rev., 14, 217, 10.1016/j.rser.2009.07.020

Chojnacka, 2004, Kinetic and stoichiometric relationships of the energy and carbon metabolism in the culture of microalgae, Biotechnology, 3, 21, 10.3923/biotech.2004.21.34

Spolaore, 2006, Commercial applications of microalgae, J. Biosci. Bioeng., 101, 87, 10.1263/jbb.101.87

Borowitzka, 2013, High-value products from microalgae—Their development and commercialisation, J. Appl. Phycol., 25, 743, 10.1007/s10811-013-9983-9

Yeo, 2018, Identification of the key structural parameters for the design of a large-scale PBR, Biosyst. Eng., 171, 165, 10.1016/j.biosystemseng.2018.04.012

Lim, 2012, Microalgal biofactories: A promising approach towards sustainable omega-3 fatty acid production, Microb. Cell Factories, 11, 96, 10.1186/1475-2859-11-96

Aramrueang, 2016, Effects of hydraulic retention time and organic loading rate on performance and stability of anaerobic digestion of Spirulina platensis, Biosyst. Eng., 147, 174, 10.1016/j.biosystemseng.2016.04.006

Chen, 2018, The potential of microalgae in biodiesel production, Renew. Sustain. Energy Rev., 90, 336, 10.1016/j.rser.2018.03.073

Barsanti, L., and Gualtieri, P. (2006). Algal culturing. Algae: Anatomy, Biochemistry, and Biotechnology Taylor and Francis, CRC Press. [1st ed.].

Pulz, 2001, Photobioreactors: Production systems for phototrophic microorganisms, Appl. Microbiol. Biotechnol., 57, 287, 10.1007/s002530100702

Seo, 2012, Numerical investigation of a bubble-column photo-bioreactor design for microalgae cultivation, Biosyst. Eng., 113, 229, 10.1016/j.biosystemseng.2012.08.001

Rösch, C., and Posten, C. (2012). Challenges and Perspectives of Microalgae Production, Karlsruhe Institute of Technology (KIT).

Metsoviti, M.N., Papapolymerou, G., Karapanagiotidis, I.T., and Katsoulas, N. (2019). Comparison of growth rate and nutrient content of five microalgae species cultivated in greenhouses. Plants, 8.

Li, 2012, Effect of light intensity on algal biomass accumulation and biodiesel production for mixotrophic strains Chlorella kessleri and Chlorella protothecoide cultivated in highly concentrated municipal wastewater, Biotechnol. Bioeng., 109, 2222, 10.1002/bit.24491

Xu, 2016, The influence of photoperiod and light intensity on the growth and photosynthesis of Dunaliella salina (chlorophyta) CCAP 19/30, Plant Physiol. Biochem., 106, 305, 10.1016/j.plaphy.2016.05.021

Singh, 2015, Effect of temperature and light on the growth of algae species: A review, Renew. Sustain. Energy Rev., 50, 431, 10.1016/j.rser.2015.05.024

Difusa, 2015, Effect of light intensity and pH condition on the growth, biomass and lipid content of microalgae Scenedesmus species, Biofuels, 6, 37, 10.1080/17597269.2015.1045274

Lee, 2015, Growth kinetic models for microalgae cultivation: A review, Algal Res., 12, 497, 10.1016/j.algal.2015.10.004

Minhas, 2016, A review on the assessment of stress conditions for simultaneous production of microalgal lipids and carotenoids, Front. Microbiol., 7, 546, 10.3389/fmicb.2016.00546

Wijffels, 2010, Microalgae for the production of bulk chemicals and biofuels, Biofuels Bioprod. Bioref., 4, 287, 10.1002/bbb.215

2018, Biodiesel synthesis from Chlorella vulgaris under effect of nitrogen limitation, intensity and quality light: Estimation on the based fatty acids profiles, Mol. Biol. Rep., 45, 1145, 10.1007/s11033-018-4266-9

Khalili, 2015, Influence of nutrients and LED light intensities on biomass production of microalgae Chlorella vulgaris, Biotechnol. Bioprocess Eng., 20, 284, 10.1007/s12257-013-0845-8

Liu, 2008, Effect of iron on growth and lipid accumulation in Chlorella vulgaris, Bioresour. Technol., 99, 4717, 10.1016/j.biortech.2007.09.073

Gouveia, 2009, Microalgae as a raw material for biofuels production, J. Ind. Microbiol. Biotechnol., 36, 269, 10.1007/s10295-008-0495-6

Metsoviti, 2019, Effect of nitrogen concentration, two-stage and prolonged cultivation on growth rate, lipid and protein content of Chlorella vulgaris, J. Chem. Technol. Biotechnol., 94, 1466, 10.1002/jctb.5899

George, 2014, Effects of different media composition, light intensity and photoperiod on morphology and physiology of freshwater microalgae Ankistrodesmus falcatus—A potential strain for bio-fuel production, Bioresour. Technol., 171, 367, 10.1016/j.biortech.2014.08.086

He, 2015, Effect of light intensity on physiological changes, carbon allocation and neutral lipid accumulation in oleaginous microalgae, Bioresour. Technol., 191, 219, 10.1016/j.biortech.2015.05.021

Ruangsomboon, 2012, Effect of light, nutrient, cultivation time and salinity on lipid production of newly isolated strain of the green microalga, Botryococcus braunii KMITL 2, Bioresour. Technol., 109, 261, 10.1016/j.biortech.2011.07.025

Baiee, 2016, Effect of phosphorus concentration and light intensity on protein content of microalga Chlorella vulgaris, Mesop. Environ. J., 2, 75

SAG. Sammlung von Algenkulturen der Universität Göttingen (2019, November 04). Culture Collection of Algae, Abteilung Experimentelle Phykologie und Sammlung von Algenkulturen (EPSAG), Universität Göttingen, Deutschland. Available online: http://epsag.uni-goettingen.de.

Chen, 2011, Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: A critical review, Bioresour. Technol., 102, 71, 10.1016/j.biortech.2010.06.159

Perez, 2017, An effective method for harvesting of marine microalgae: pH induced flocculation, Bioresour. Technol., 97, 20

Safi, 2014, Morphology, composition, production, processing and applications of Chlorella vulgaris: A review, Renew. Sustain. Energy Rev., 35, 265, 10.1016/j.rser.2014.04.007

Association of Official Analytical Chemists (AOAC) (1995). Official Methods of Analysis of the Association of Official Analytical Chemists International, AOAC. [16th ed.].

Biancarosa, 2017, Amino acid composition, protein content, and nitrogen-to-protein conversion factors of 21 seaweed species from Norwegian waters, J. Appl. Phycol., 29, 1001, 10.1007/s10811-016-0984-3

Folch, 1957, A simple method for the isolation and purification of total lipides from animal tissues, J. Biol. Chem., 226, 497, 10.1016/S0021-9258(18)64849-5

Ryckebosch, 2012, Optimization of an analytical procedure for extraction of lipids from microalgae, J. Am. Oil Chem. Soc., 89, 189, 10.1007/s11746-011-1903-z

Dean, 2010, Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae, Bioresour. Technol., 101, 4499, 10.1016/j.biortech.2010.01.065

Sharma, 2012, Effects of culture conditions on growth and biochemical profile of Chlorella vulgaris, J. Plant Pathol. Microb., 3, 5, 10.4172/2157-7471.1000131

Daliry, 2017, Investigation of optimal condition for Chlorella vulgaris microalgae growth, Glob. J. Environ. Sci. Manag., 3, 217

Khoeyi, 2010, Effect of light intensity and photoperiod on biomass and fatty acid composition of the microalgae, Chlorella vulgaris, Aquac. Int., 20, 41, 10.1007/s10499-011-9440-1

Seyfabadi, 2011, Protein, fatty acid, and pigment content of Chlorella vulgaris under different light regimes, J. Appl. Phycol., 23, 721, 10.1007/s10811-010-9569-8

Lichtenthaler, 1987, Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes, Methods Enzymol., 148, 350, 10.1016/0076-6879(87)48036-1

Das, 2011, Enhanced algae growth in both phototrophic and mixotrophic culture under blue light, Bioresour. Technol., 102, 3883, 10.1016/j.biortech.2010.11.102

Ra, 2016, Effects of light-emitting diodes (LEDs) on the accumulation of lipid content using a two-phase culture process with three microalgae, Bioresour. Technol., 212, 254, 10.1016/j.biortech.2016.04.059

Blair, 2014, Light and growth medium effect on Chlorella vulgaris biomass production, J. Environ. Chem. Eng., 2, 665, 10.1016/j.jece.2013.11.005

Wong, 2016, Effect of different light sources on algal biomass and lipid production in internal Leds-illuminated photobioreactor, J. Mar. Biol. Aquac., 2, 1

Atta, 2013, Intensity of blue LED light: A potential stimulus for biomass and lipid content in fresh water microalgae Chlorella vulgaris, Bioresour. Technol., 148, 373, 10.1016/j.biortech.2013.08.162

Rubio, 2002, A mechanistic model of photosynthesis in microalgae, Bioresour. Technol., 81, 459

Blanken, 2016, Predicting microalgae growth, Algal Res., 14, 28, 10.1016/j.algal.2015.12.020

Takache, 2012, Kinetic modeling of the photosynthetic growth of Chlamydomonas reinhardtii in a photobioreactor, Biotechnol. Prog., 28, 681, 10.1002/btpr.1545

Sasi, 2011, Growth kinetics and lipid production using Chlorella vulgaris in a circulating loop photobioreactor, J. Chem. Technol. Biotechnol., 86, 875, 10.1002/jctb.2603

Solovchenko, 2008, Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa, J. Phycol., 20, 245, 10.1007/s10811-007-9233-0

Liu, 2012, Effects of light intensity on the growth and lipid accumulation of microalga Scenedesmus sp. 11-1 under nitrogen limitation, Appl. Biochem. Biotechnol., 166, 2127, 10.1007/s12010-012-9639-2

Xia, 2013, Effects of nutrients and light intensity on the growth and biochemical composition of a marine microalga Odontella aurita, Chin. J. Oceanol. Limnol., 3, 1

Pruvost, 2011, Systematic investigation of biomass and lipid productivity by microalgae in photobioreactors for biodiesel application, Bioresour. Technol., 102, 150, 10.1016/j.biortech.2010.06.153

Zhang, Y., Dong, F., and Jin, P. (2017, January 23–25). Effects of Light-emitting Diodes (LEDs) on the Accumulation of Lipid Content in Microalgae. Proceedings of the 2nd International Conference on Sustainable Energy and Environment Protection (ICSEEP 2017), Changsha, China.

2016, Effect of green and red light in lipid accumulation and transcriptional profile of genes implicated in lipid biosynthesis in Chlamydomonas reinhardtii, Biotechnol. Prog., 32, 1404, 10.1002/btpr.2368

Kendirlioglu, 2017, Effect of different wavelengths of light on growth, pigment content and protein amount of Chlorella vulgaris, Fresenius Environ. Bull., 26, 7974

Asuthkar, 2016, Effect of different wavelengths of light on the growth of Chlorella pyrenoidosa, Int. J. Pharm. Sci. Res., 7, 847

Thông tin
Thông tin xuất bản

Plants

Tập 9 Số 1

31

Thông tin tác giả