Effect of FPZ, a total flavonoids ointment topical application from Pouzolzia zeylanica var. microphylla, on mice skin infections
Tóm tắt
The present study was designed to investigate the effect of FPZ, a total flavonoids ointment topical application from Pouzolzia zeylanica var. microphylla (Wedd.) Masam, Urticaceae, on skin infections in mice. FPZ ointment anti-infective effect was investigated on Staphylococcus aureus-induced skin abscess and skin ulcers in mice by evaluating the variation in abscess volume, histopathology of skin tissue and healing rate. Secondary, it is topical anti-inflammatory activities on carrageenan-induced hind paw edema in mice was estimated. Besides, FPZ ointment fingerprint was performed by using ultra-performance liquid chromatography and FPZ ointment chemical constituents were isolated and identified by repeated column chromatograph and spectroscopic methods. The results revealed that FPZ ointment topical application at the concentration of 2.5-10% could attenuate skin abscess and ulcers and accelerate wound healing, as compared with control group treated with vehicle (p<0.05). The histological analysis indicated that FPZ ointment acted via inflammation inhibition, granulation promotion and epidermis formation. Moreover, FPZ ointment effectively inhibited carrageenan-induced paw edema in a dose-dependent manner, especially 10% FPZ which showed superior activities in comparison with dexamethasone used as reference drug. FPZ ointment topical application showed a significant anti-infective effect against pyogenic bacterial skin infection in mice.
Tài liệu tham khảo
Ahmed, A., Rajendaran, K., Jaiswal, D., Singh, H.P., Mishra, A., Chandra, D., Yadav, I.K., Jain, D.A., 2010. Anti-snake venom activity of different extracts of Pouzolzia indica against Russel viper venom. Int. J. ChemTech Res. 2, 744–751.
Araujo, L.U., Grabe-Guimaraes, A., Mosqueira, V.C., Carneiro, C.M., Silva-Barcellos, N.M., 2010. Profile of wound healing process induced by allantoin. Acta Cir. Bras. 25, 460–466.
Chen, Z.H., 2016. The preparation of Pouzolzia zeylanica (L.) Benn. var. microphylla (Wedd.) W.T. Wang. Ointment. MSc dissertation, Guangdong Pharmaceutical University.
Dangol, D.R., Gurung, S.B., 1991. Ethnobotany of the Tharu Tribeof Chitwan District, Nepal. Pharm. Biol. 29, 203–209.
Di Carlo, G., Mascolo, N., Izzo, A.A., Capasso, F., 1999. Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sci. 65, 337–353.
Guo, L.B., 2011. Application of Pouzolzia extract. China Patent, 201110338498.
Li, K.Y., Xie, Y.F., Chen, Y.F., Liu, T.Z., Guo, LB., 2012. Effect of Pouzolzia zeylanica extracts in mouse subcutaneous abscess. J. Guangdong Pharm. Univ. 28, 540–544.
Li, K.Y., 2014. Study on antiinflammatory ingredients and mechanisms of Pouzolzia zeylanica var. microphylla. MSc dissertation, Guangdong Pharmaceutical University.
Li, Z.H., Chen, X.M., Tao, S.H., Zhong, C.Q., Guo, L.B., 2017. Study on chemical cosstituents from Pouzolzia zeylanica (III). J. Guangdong Pharm. Univ. 33, 177–180.
Liu, X.Y., Du, Q.T., Li, K.Y., Deng, Q., Guo, L.B., 2012. Antiinflammatory and analgesic effects of different extract fractions from Pouzolzia zeylanica var. microphylla. Drugs Clinic 27, 356–369.
Liu, X.Y., Xie, Y.F., Zhang, H., Liu, T.Z., Wen, T., Guo, L.B., 2014. Chemical constituents from Pouzolzia zeylanica (L) Benn. var. microphylla (Wedd.) W.T Wang. (I). J. Guangdong Pharm. Univ. 20, 43–47.
Luo, Z., Zhong, C.Q., Liu, X.Y., Yi, Z.B., Guo, L.B., 2014. Study on chemical constituents from Pouzolzia zeylanica (II). J. Guangdong Pharm. Univ. 30, 430–434.
Malachowa, N., Kobayashi, S., Braughton, K., DeLeo, F., 2013. Mouse model of Staphylococcus aureus skin infection. In: Allen, I.C. (Ed.), Mouse Models of Innate Immunity. Humana Press, pp. 109–116.
Martinez, L.R., Han, G., Chacko, M., Mihu, M.R., Jacobson, M., Gialanella, P., Friedman, A.J., Nosanchuk, J.D., Friedman, J.M., 2009. Antimicrobial and healing efficacy of sustained release nitric oxide nanoparticles against Staphylococcus aureus skin infection. J. Investig. Dermatol. 129, 2463–2469.
Matsuda, H., Ninomiya, K., Shimoda, H., Yoshikawa, M., 2002. Hepatoprotective principles from the flowers of Tilia argentea (Linden): structure requirements of tiliroside and mechanisms of action. Bioorg. Med. Chem. 10, 707–712.
Pan, J.S., Hong, M.Z., Ren, J.L., 2009. Reactive oxygen species: a double-edged sword in oncogenesis. World J. Gastroenterol. 15, 1702–1707.
Rabe, T., van Staden, J., 1997. Antibacterial activity of South African plants used for medicinal purposes. J. Ethnopharmacol. 56, 81–87.
Siriwatanametanon, N., Fiebich, B.L., Efferth, T., Prieto, J.M., Heinrich, M., 2010. Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities. J. Ethnopharmacol. 130, 196–207.
Toker, G., Kuepeli, E., Memisoglu, M., Yesilada, E., 2004. Flavonoids with antinociceptive and anti-inflammatory activities from the leaves of Tilia argentea (silver linden). J. Ethnopharmacol. 95, 393–397.
Van Sam, H., Baas, P., Keßler, P.J.A., 2008. Traditional medicinal plants in Ben En National Park Vietnam. Blumea 53, 569–601.
VinodKumar, C.S., Srinivasa, H., Basavarajappa, K.G., Patil, U., Bandekar, N., Patil, R., 2011. Abrogation of Staphylococcus aureus wound infection by bacteriophage in diabetic rats. Int. Pharm. Sci. Drug Res. 3, 202–207.
Wilgus, T.A., 2008. Immune cells in the healing skin wound: influential players at each stage of repair. Pharmacol. Res. 58, 112–116.
Yadav, I.K., Singh, H.P., Jain, D.A., Ahmed, A., Indranil, D., 2012. In vitro antioxidant and radical scavenging activity of different extracts of Pouzolzia indica. Elixir Int. J. 47, 9143–9148.
Yin, H., Li, X., Hu, S., 2013. IL-33 promotes Staphylococcus aureus-infected wound healing in mice. Int. Immunopharmacol. 17, 432–438.
Yu, J.K., Lu, Y.Y., Li, Y.P., Xiao, L.L., Xing, Y., Li, Y.S., Wu, L.M., 2015. Roleof S100A1 in hypoxia-induced inflammatory response in cardiomyocytes via TLR4/ROS/NF-κB pathway. J. Pharm. Pharmacol. 67, 1240–1250.
Zhang, T.T., Wang, M., Yang, L., Jiang, J.G., Zhao, J.W., Zhu, W., 2015. Flavonoid glycosides from Rubus chingii Hu fruits display anti-inflammatory activity through suppressing MAPKs activation in macrophages. J. Funct. Food 18 (Part A), 235–243.