Effect of Extraction Solvent/Technique on the Antioxidant Activity of Selected Medicinal Plant Extracts
Tóm tắt
Theeffects of four extracting solvents [absolute ethanol, absolute methanol, aqueous ethanol (ethanol: water, 80:20 v/v) and aqueous methanol (methanol: water, 80:20 v/v)] and two extraction techniques (shaking and reflux) on the antioxidant activity of extracts of barks of Azadirachta indica, Acacia nilotica, Eugenia jambolana, Terminalia arjuna, leaves and roots of Moringa oleifera, fruit of Ficus religiosa,and leaves of Aloe barbadensis were investigated. The tested plant materials contained appreciable amounts of total phenolic contents (0.31-16.5 g GAE /100g DW), total flavonoid (2.63-8.66 g CE/100g DW); reducing power at 10 mg/mL extract concentration (1.36-2.91), DPPH. scavenging capacity (37.2-86.6%), and percent inhibition of linoleic acid (66.0-90.6%). Generally higher extract yields, phenolic contents and plant material antioxidant activity were obtained using aqueous organic solvents, as compared to the respective absolute organic solvents. Although higher extract yields were obtained by the refluxing extraction technique, in general higher amounts of total phenolic contents and better antioxidant activity were found in the extracts prepared using a shaker.
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Tài liệu tham khảo
Urooj, 2007, Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves, Food Chem., 102, 1233, 10.1016/j.foodchem.2006.07.013
Antolovich, 2000, Sample preparation in the determination of phenolic compounds in fruits, Analyst, 125, 989, 10.1039/b000080i
Peschel, 2006, An industrial approach in the search of natural antioxidants from vegetable and fruit wastes, Food Chem., 97, 137, 10.1016/j.foodchem.2005.03.033
Abdille, 2005, Antioxidant activity of the extracts from Dillenia indica fruits, Food Chem., 90, 891, 10.1016/j.foodchem.2004.09.002
Rehman, 2006, Citrus peel extract- A natural source of antioxidant, Food Chem., 99, 450, 10.1016/j.foodchem.2005.07.054
Li, 2006, Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract, Food Chem., 96, 254, 10.1016/j.foodchem.2005.02.033
Bonoli, 2004, Antioxidant phenols in barley (Hordeum vulgare L.) flour: comparative spectrophotometric study among extraction methods of free and bound phenolic acids, J. Agric. Food Chem., 52, 5195, 10.1021/jf040075c
Chatha, 2006, Evaluation of the antioxidant activity of rice bran extracts using different antioxidant assays, Grasas Aceites Sevilla, 57, 328
Siddhuraju, 2003, Antioxidant properties of various extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera lam.) leaves, J. Agric. Food Chem., 51, 2144, 10.1021/jf020444+
Anwar, 2006, Antioxidant activity of various plant extracts under ambient and accelerated storage of sunflower oil, Grasas Aceites Sevilla, 57, 189
Hu, 2003, u, Q, Evaluation of Antioxidant Potential of Aloe vera (Aloe barbadensis Miller) Extracts, J. Agric. Food Chem., 51, 7788, 10.1021/jf034255i
Dwievedi, 2007, Terminalia arjuna Wight & Arn.-A useful drug for cardiovascular disorders, J. Ethnopharm., 114, 114, 10.1016/j.jep.2007.08.003
Sultana, 2008, Flavonols (kaempferol, quercetin, myricetin) contents of selected fruits, vegetables and medicinal plants, Food Chem., 108, 879, 10.1016/j.foodchem.2007.11.053
Hsu, 2006, Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica, Food Chem., 98, 317, 10.1016/j.foodchem.2005.05.077
Sultana, 2007, Antioxidant activity of phenolic components present in barks of barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees, Food Chem., 104, 1106, 10.1016/j.foodchem.2007.01.019
Shon, 2004, Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onion, Food Chem. Toxicol., 42, 659, 10.1016/j.fct.2003.12.002
Tung, 2007, Antioxidant activities of natural phenolic compounds from Acacia confusa bark, Biores. Technol., 98, 1120, 10.1016/j.biortech.2006.04.017
Yu, 2008, Antioxidant, immunomodulatory and anti-breast cancer activities of phenolic extract from pine (Pinus massoniana Lamb) bark, Inno. Food Sci. Emer. Technol., 9, 122, 10.1016/j.ifset.2007.06.006
Iqbal, 2006, Effect of season and production location on the antioxidant activity of Moringa oleifera leaves grown in Pakistan, J. Food Comp. Anal., 102, 544, 10.1016/j.jfca.2005.05.001
Liu, 2008, Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medicinal or food uses, Food Res. Inter., 41, 363, 10.1016/j.foodres.2007.12.012
Ao, 2008, Evaluation of antioxidant and antimicrobial activities of Ficus microcarpa L. fil. extract, Food Contr., 19, 940, 10.1016/j.foodcont.2007.09.007
Dekker, 2005, Activity and concentration of polyphenolic antioxidants in apple juice. 3. stability during storage, J. Agric. Food Chem., 53, 1073, 10.1021/jf040270r
Cheng, 2006, Effect of postharvest treatment and heat stress on availability of wheat antioxidants, J. Agric. Food Chem., 54, 5623, 10.1021/jf060719b
Dutra, 2008, Quantification of phenolic constituents and antioxidant activity of Pterodon emarginatus vogel seeds, Inter. J. Mol. Sci., 9, 606, 10.3390/ijms9040606
Chaovanalikit, 2004, Total anthocyanins and total phenolics of fresh and processed cherries and their antioxidant properties, J. Food Sci., 69, 67, 10.1111/j.1365-2621.2004.tb17858.x
Dewanto, 2002, Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity, J. Agric. Food Chem., 50, 3010, 10.1021/jf0115589
Yen, 2000, Antioxidant activity of anthraquinones and anthrone, Food Chem., 70, 307, 10.1016/S0308-8146(00)00108-4