Effect of Drought Stress on Chlorophyll Fluorescence Parameters, Phytochemical Contents, and Antioxidant Activities in Lettuce Seedlings

Horticulturae - Tập 7 Số 8 - Trang 238
Yu Kyeong Shin1, Shiva Ram Bhandari2,1, Jung Su Jo1, Jae Woo Song1, Jun Gu Lee2,1,3
1Department of Horticulture, College of Agriculture & Life Sciences, Jeonbuk National University, Jeonju 54896, Korea
2Core Research Institute of Intelligent Robots, Jeonbuk National University, Jeonju 54896, Korea
3Institute of Agricultural Science & Technology, Jeonbuk National University, Jeonju 54896, Korea

Tóm tắt

This study monitored changes in chlorophyll fluorescence (CF), growth parameters, soil moisture content, phytochemical content (proline, ascorbic acid, chlorophyll, total phenol content (TPC), and total flavonoid content (TFC)), and antioxidant activities in 12-day-old lettuce (Lactuca sativa L.) seedlings grown under drought stress (no irrigation) and control (well irrigated) treatments in controlled conditions for eight days. Measurements occurred at two-day intervals. Among ten CF parameters studied, effective quantum yield of photochemical energy conversion in PSII (Y(PSII)), coefficient of photochemical quenching (qP), and coefficient of photochemical quenching of variable fluorescence based on the lake model of PSII (qL) significantly decreased in drought-stressed seedlings from day 6 of treatment compared to control. In contrast, maximum quantum yield (Fv/Fm), ratio of fluorescence (Rfd), and quantum yield of non-regulated energy dissipation in PSII (Y(NO)) were significantly affected only at the end. All growth parameters decreased in drought-stressed seedlings compared to control. Proline started increasing from day 4 and showed ~660-fold elevation on day 8 compared to control. Chlorophyll, ascorbic acid, TPC, TFC, and antioxidant activities decreased in drought-stressed seedlings. Results showed major changes in all parameters in seedlings under prolonged drought stress. These findings clarify effects of drought stress in lettuce seedlings during progressive drought exposure and will be useful in the seedling industry.

Từ khóa


Tài liệu tham khảo

FAO (Food and Agriculture Organization) (2021, May 25). Agricultural Statistical Database for 2019. Available online: http://www.fao.org/faostat/en/#data/QC.

Mulabagal, 2010, In vitro evaluation of red and green lettuce (Lactuca sativa) for functional food properties, Food Chem., 118, 300, 10.1016/j.foodchem.2009.04.119

Llorach, 2008, Characterisation of polyphenols and antioxidant properties of five lettuce varieties and escarole, Food Chem., 108, 1028, 10.1016/j.foodchem.2007.11.032

Zlotek, 2014, Effect of abiotic elicitation on main health-promoting compounds, antioxidant activity and commercial quality of butter lettuce (Lactuca sativa L.), Food Chem., 148, 253, 10.1016/j.foodchem.2013.10.031

Camejo, 2020, Artificial light impacts the physical and nutritional quality of lettuce, Hortic. Environ. Biotechnol., 61, 69, 10.1007/s13580-019-00191-z

Carotti, 2020, Plant factories are heating up: Hunting for the best combination of light intensity, air temperature and root-zone temperature in lettuce production, Front. Plant Sci., 11, 2251

Sofo, 2016, Different agronomic and fertilization systems affect polyphenolic profile, antioxidant capacity and mineral composition of lettuce, Sci. Hortic., 204, 106, 10.1016/j.scienta.2016.04.003

Bilalis, 2009, Comparison between conventional and organic floating systems for lettuce and tomato (Lactuca sativa and Lycopersicon esculentum) seedling production, J. Food Agric. Environ., 7, 623

Johkan, 2010, Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce, HortScience, 45, 1809, 10.21273/HORTSCI.45.12.1809

Fereres, 2003, Irrigation water management of horticultural crops, HortScience, 38, 1036, 10.21273/HORTSCI.38.5.1036

Kurunc, A. (2021). Effects of water and salinity stresses on growth, yield, and water use of iceberg lettuce. J. Sci. Food Agric.

Dessart, 2019, Behavioural factors affecting the adoption of sustainable farming practices: A policy-oriented review, Eur. Rev. Agric. Econ., 46, 417, 10.1093/erae/jbz019

Fereres, 2012, Combining the simulation crop model aqua crop with an economic model for the optimization of irrigation management at farm level, Eur. J. Agron., 36, 21, 10.1016/j.eja.2011.08.003

Yuan, 2010, Effect of brassinosteroids on drought resistance and abscisic acid concentration in tomato under water stress, Sci. Hortic., 126, 103, 10.1016/j.scienta.2010.06.014

Zargar, 2017, Impact of drought on photosynthesis: Molecular perspective, Plant Gene, 11, 154, 10.1016/j.plgene.2017.04.003

Franzoni, 2021, Effect of glutamic acid foliar applications on lettuce under water stress, Physiol. Mol. Biol. Plants, 27, 1059, 10.1007/s12298-021-00984-6

Pervez, 2009, Effect of drought stress on growth, yield and seed quality of tomato (Lycopersicon esculentum L.), Pak. J. Agric. Sci., 46, 174

Liang, 2020, Effects of drought stress on photosynthetic and physiological parameters of tomato, J. Am. Soc. Hortic. Sci., 145, 12, 10.21273/JASHS04725-19

Paim, 2020, Mild drought stress has potential to improve lettuce yield and quality, Sci. Hortic., 272, 109578, 10.1016/j.scienta.2020.109578

Zhou, 2017, Drought stress had a predominant effect over heat stress on three tomato cultivars subjected to combined stress, BMC Plant Biol., 17, 1, 10.1186/s12870-017-0974-x

Yadav, 2021, Secondary metabolites in the drought stress tolerance of crop plants: A review, Gene Rep., 23, 101040, 10.1016/j.genrep.2021.101040

Akram, 2017, Ascorbic acid-a potential oxidant scavenger and its role in plant development and abiotic stress tolerance, Front. Plant Sci., 8, 613, 10.3389/fpls.2017.00613

Chun, 2018, Proline accumulation influenced by osmotic stress in arbuscular mycorrhizal symbiotic plants, Front. Plant Sci., 9, 2525

Ahmed, 2013, Genotypic differences in physiological characteristics in the tolerance to drought and salinity combined stress between Tibetan wild and cultivated barley, Plant Physiol. Biochem., 63, 49, 10.1016/j.plaphy.2012.11.004

Kabiri, 2014, Effect of exogenous salicylic acid on some physiological parameters and alleviation of drought stress in Nigella sativa plant under hydroponic culture, Plant Prot. Sci., 50, 43, 10.17221/56/2012-PPS

Kiran, 2019, Effects of vermicompost on some morphological, physiological and biochemical parameters of lettuce (lactuca sativa var. crispa) under drought stress, Not. Bot. Horti Agrobot. Cluj Napoca, 47, 352, 10.15835/nbha47111260

Klunklin, W., and Savage, G. (2017). Effect on quality characteristics of tomatoes grown under well-watered and drought stress conditions. Foods, 6.

Kopta, T., Sekara, A., Pokluda, R., Ferby, V., and Caruso, G. (2020). Screening of chilli pepper genotypes as a source of capsaicinoids and antioxidants under conditions of simulated drought stress. Plants, 9.

Ors, 2021, Interactive effects of salinity and drought stress on photosynthetic characteristics and physiology of tomato (Lycopersicon esculentum L.) seedlings, S. Afr. J. Bot., 137, 335, 10.1016/j.sajb.2020.10.031

Yao, 2018, Phenotyping of Arabidopsis drought stress response using kinetic chlorophyll fluorescence and multicolor fluorescence imaging, Front. Plant Sci., 9, 603, 10.3389/fpls.2018.00603

Yoon, H.I., Zhang, W., and Son, J.E. (2020). Optimal duration of drought stress near harvest for promoting bioactive compounds and antioxidant capacity in kale with or without UV-B radiation in plant factories. Plants, 9.

Gorbe, 2012, Applications of chlorophyll fluorescence imaging technique in horticultural research: A review, Sci. Hortic., 138, 24, 10.1016/j.scienta.2012.02.002

Susic, 2018, Discrimination between abiotic and biotic drought stress in tomatoes using hyperspectral imaging, Sens. Actuators B Chem., 273, 842, 10.1016/j.snb.2018.06.121

Murchie, 2013, Chlorophyll fluorescence analysis: A guide to good practice and understanding some new applications, J. Exp. Bot., 64, 3983, 10.1093/jxb/ert208

Naderi, 2020, Tolerance responses in wheat landrace Bolani are related to enhanced metabolic adjustments under drought stress, Plant Physiol. Biochem., 150, 244, 10.1016/j.plaphy.2020.03.002

Seminario, 2017, Drought stress causes a reduction in the biosynthesis of ascorbic acid in soybean plants, Front. Plant Sci., 8, 1042, 10.3389/fpls.2017.01042

Xu, Q., Ma, X., Lv, T., Bai, M., Wang, Z., and Niu, J. (2020). Effects of water stress on fluorescence parameters and photosynthetic characteristics of drip irrigation in rice. Water, 12.

Oh, 2010, Regulated water deficits improve phytochemical concentration in lettuce, J. Am. Soc. Hortic. Sci., 135, 223, 10.21273/JASHS.135.3.223

Shin, Y.K., Bhandari, S.R., Jo, J.S., Song, J.W., Cho, M.C., Yang, E.Y., and Lee, J.G. (2020). Response of salt stress in lettuce: Changes in chlorophyll fluorescence parameters, phytochemical contents, and antioxidant activities. Agronomy, 10.

Bhandari, 2016, Ripening-dependent changes in antioxidants, color attributes, and antioxidant activity of seven tomato (Solanum lycopersicum L.) cultivars, J. Anal. Method Chem., 2016, 5498618, 10.1155/2016/5498618

Bhandari, 2016, Genotypic variation in carotenoid, ascorbic acid, total phenolic, and flavonoid contents, and antioxidant activity in selected tomato breeding lines, Hortic. Environ. Biotechnol., 57, 440, 10.1007/s13580-016-0144-3

Dodd, 2010, Root water potential integrates discrete soil physical properties to influence ABA signaling during partial rootzone drying, J. Exp. Bot., 61, 3543, 10.1093/jxb/erq195

Ghanbarzadeh, 2021, Rhizosphere symbionts improve water stress tolerance in moldavian balm through modulation of osmolytes, Rhizosphere, 19, 100367, 10.1016/j.rhisph.2021.100367

Inoue, 2021, Minimizing VPD fluctuations maintains higher stomatal conductance and photosynthesis, resulting in improvement of plant growth in lettuce, Front. Plant Sci., 12, 458, 10.3389/fpls.2021.646144

Banks, 2018, Chlorophyll fluorescence as a tool to identify drought stress in Acer genotypes, Environ. Exp. Bot., 155, 118, 10.1016/j.envexpbot.2018.06.022

Xu, 2015, Effects of A. nodosum seaweed extracts on spinach growth, physiology and nutrition value under drought stress, Sci. Hortic., 183, 39, 10.1016/j.scienta.2014.12.004

Parkash, V., and Singh, S. (2020). A review on potential plant-based water stress indicators for vegetable crops. Sustainability, 12.

Magalhaes, 2019, Action of N-Succinyl and N, O-Dicarboxymethyl chitosan derivatives on chlorophyll photosynthesis and fluorescence in drought-sensitive maize, J. Plant Growth Regul., 38, 619, 10.1007/s00344-018-9877-9

Huang, 2019, Exogenous melatonin alleviates oxidative damages and protects photosystem II in maize seedlings under drought stress, Front. Plant Sci., 10, 677, 10.3389/fpls.2019.00677

Sun, D., Zhu, Y., Xu, H., He, Y., and Cen, H. (2019). Time-series chlorophyll fluorescence imaging reveals dynamic photosynthetic fingerprints of sos mutants to drought stress. Sensors, 19.

Genty, 1989, The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence, Biochim. Biophys. Acta, 990, 87, 10.1016/S0304-4165(89)80016-9

Anderson, 1995, The grand design of photosynthesis: Acclimation of the photosynthetic apparatus to environmental cues, Photosynth. Res., 46, 129, 10.1007/BF00020423

Ruban, 2012, Assessing the photoprotective effectiveness of non-photochemical chlorophyll fluorescence quenching: A new approach, Biochim. Biophys. Acta, 1817, 977, 10.1016/j.bbabio.2012.03.026

Adams, 1996, III. The role of xanthophyll cycle carotenoids in the protection of photosynthesis, Trends Plant Sci., 1, 21, 10.1016/S1360-1385(96)80019-7

Wang, 2018, Effect of drought stress on photosynthesis and photosynthetic electron transport chain in young apple tree leaves, Biol. Open, 7, bio35279

Pinnola, 2013, Zeaxanthin binds to light-harvesting complex stress-related protein to enhance nonphotochemical quenching in Physcomitrella patens, Plant Cell, 25, 3519, 10.1105/tpc.113.114538

Ruban, 2016, Nonphotochemical chlorophyll fluorescence quenching: Mechanism and effectiveness in protecting plants from photodamage, Plant Physiol., 170, 1903, 10.1104/pp.15.01935

Kapoor, D., Bhardwaj, S., Landi, M., Sharma, A., Ramakrishnan, M., and Sharma, A. (2020). The impact of drought in plant metabolism: How to exploit tolerance mechanisms to increase crop production. Appl. Sci., 10.

Nikolaeva, 2010, Effect of drought on chlorophyll content and antioxidant enzyme activities in leaves of three wheat cultivars varying in productivity, Russ. J. Plant Physiol., 57, 87, 10.1134/S1021443710010127

Gupta, 2014, Mechanism of salinity tolerance in plants: Physiological, biochemical, and molecular characterization, Int. J. Genom., 2014, 701596

Trovato, 2008, Multiple role of proline in plant stress tolerance and development, Rend. Lincei, 19, 325, 10.1007/s12210-008-0022-8

Sahitya, 2018, Seed antioxidants interplay with drought stress tolerance indices in chilli (Capsicum annuum L.) seedlings, BioMed Res. Int., 2018, 1605096, 10.1155/2018/1605096

Yazdanpanah, 2011, The interaction between drought stress and salicylic and ascorbic acids on some biochemical characteristics of Satureja hortensis, Afr. J. Agric. Res., 6, 798

Zhang, 2012, The ethylene response factor AtERF98 enhances tolerance to salt through the transcriptional activation of ascorbic acid synthesis in Arabidopsis, Plant J., 71, 273, 10.1111/j.1365-313X.2012.04996.x

Sarker, 2018, Drought stress enhances nutritional and bioactive compounds, phenolic acids and antioxidant capacity of Amaranthus leafy vegetable, BMC Plant Biol., 18, 1, 10.1186/s12870-018-1484-1