Early stage evaluation of colon cancer using tungsten disulfide quantum dots and bacteriophage nano-biocomposite as an efficient electrochemical platform

Cancer Nanotechnology - Tập 13 Số 1 - 2022
Ramin Pourakbari1, Mehdi Yousefi2, Balal Khalilzadeh3, Mahsa Haddad Irani-nezhad4, Alireza Khataee4, Leili Aghebati‐Maleki5, Alireza Soleimanian6, Amin Kamrani3, Forough Chakari-Khiavi6, Rozita Abolhasan3, Morteza Motallebnezhad7, Farhad Jadidi‐Niaragh8, Bahman Yousefi9, Hossein Samadi Kafil9, Mohammad Hojjat‐Farsangi10, Mohammad‐Reza Rashidi6
1Student's Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
2Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3Stem Cell Research Center (SCRC), Tabriz University of Medical Sciences, 51664-14766, Tabriz, Iran
4Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran
5Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
6Pharmaceutical Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
7Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
8Molecular Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
9Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
10Department of Oncology-Pathology, Immune and Gene therapy Lab, Cancer Center Karolinska (CCK), Karolinska University Hospital Solna and Karolinska Institute, Stockholm, Sweden

Tóm tắt

AbstractBackgroundRecently, biosensors have become popular analytical tools for small analytes due to their high sensitivity and wide analytical range. In the present work, development of a novel biosensing method based on tungsten disulfide quantum dots (WS2QDs)-Au for rapidly and selectively detecting c-Met protein is introduced. As a proof of concept, M13 bacteriophage-based biosensors were used for the electrochemical detection of c-Met protein as a colon cancer biomarker.MethodThe M13 bacteriophage (virus), as the biorecognition element, was immobilized on glassy carbon electrodes which were modified by WS2QDs-functionalized gold nanoparticles. The stepwise presence of the WS2QDs, gold nanoparticles, and immobilized phage on glassy carbon electrodes were confirmed by scanning electron microscope (SEM) and square wave voltammetry (SWV) technique.ResultsThe designed biosensor was applied to measure the amount of c-Met protein in standard solutions, and consequently the desirable detection limit of 1 pg was obtained. Finally, as a proof of concept, the developed platform was used for the evaluation of c-Met protein in serum samples of colon cancer-suffering patients and the results were compared with the results of the common Elisa kit.ConclusionsAs an interesting part of this study, some concentrations of the c-Met protein in colon cancer serum samples which could not be determined by Elisa, were easily analyzed by the developed bioassay system. The developed bioassay system has great potential to application in biomedical laboratories.Graphical Abstract

Từ khóa


Tài liệu tham khảo

Bera D, Qian L, Tseng T-K, Holloway PH (2010) Quantum dots and their multimodal applications: a review. Materials 3(4):2260–2345

Birchmeier C, Birchmeier W, Gherardi E, Woude GFV (2003) Met, metastasis, motility and more. Nat Rev Mol Cell Biol 4(12):915–925

Chiang H-C, Wang Y, Zhang Q, Levon K (2019) Optimization of the electrodeposition of gold nanoparticles for the application of highly sensitive. Label Free Biosens Biosens 9(2):50

Ertürk G, Lood R (2018) Bacteriophages as biorecognition elements in capacitive biosensors: phage and host bacteria detection. Sens Actuators B Chem 258:535–543

Fitzgerald RC (2020) Big data is crucial to the early detection of cancer. Nat Med 26(1):19–20

Gervais L, Gel M, Allain B, Tolba M, Brovko L, Zourob M, Mandeville R, Griffiths M, Evoy S (2007) Immobilization of biotinylated bacteriophages on biosensor surfaces. Sens Actuators B Chem 125(2):615–621

Grossman S, Milos ML, Tekawa IS, Jewell NP (1989) Colonoscopic screening of persons with suspected risk factors far colon, cancer: ii. Past history of colorectal neoplasms. Gastroenterology 96(2):299–306

Gupta S, Halm EA, Rockey DC, Hammons M, Koch M, Carter E, Valdez L, Tong L, Ahn C, Kashner M (2013) Comparative effectiveness of fecal immunochemical test outreach, colonoscopy outreach, and usual care for boosting colorectal cancer screening among the underserved: a randomized clinical trial. JAMA Intern Med 173(18):1725–1732

Haddad Irani-nezhad M, Khataee A, Hassanzadeh J, Orooji Y (2019) A chemiluminescent method for the detection of H2O2 and glucose based on intrinsic peroxidase-like activity of WS2 quantum dots. Molecules 24(4):689

Hasanzadeh M, Solhi E, Jafari M, Mokhtarzadeh A, Soleymani J, Jouyban A, Mahboob S (2018) Ultrasensitive immunoassay of tumor protein CA 15.3 in MCF-7 breast cancer cell lysates and unprocessed human plasma using gold nanoparticles doped on the structure of mesoporous silica. Int J Biol Macromol 120:2493–2508

Isildak I, Navaeipour F, Afsharan H, Kanberoglu GS, Agir I, Ozer T, Annabi N, Totu EE, Khalilzadeh B (2020) Electrochemiluminescence methods using CdS quantum dots in aptamer-based thrombin biosensors: a comparative study. Microchim Acta 187(1):1–13

Jafarizad A, Taghizadehgh-Alehjougi A, Eskandani M, Hatamzadeh M, Abbasian M, Mohammad-Rezaei R, Mohammadzadeh M, Toğar B, Jaymand M (2018) PEGylated graphene oxide/Fe3O4 nanocomposite: synthesis, characterization, and evaluation of its performance as de novo drug delivery nanosystem. Bio Med Mater Eng 29(2):177–190

Jalili R, Khataee A (2019) Aluminum (III) triggered aggregation-induced emission of glutathione-capped copper nanoclusters as a fluorescent probe for creatinine. Microchim Acta 186(1):29

Jaymand M (2011) Synthesis and characterization of an exfoliated modified syndiotactic polystyrene/Mg–Al-layered double-hydroxide nanocomposite. Polym J 43(2):186–193

Khalilzadeh B, Hasanzadeh M, Sanati S, Saghatforoush L, Shadjou N, Dolatabadi JEN, Sheikhzadeh P (2011) Preparation of a new electrochemical sensor based on cadmium oxide nanoparticles and application for determination of penicillamine. Int J Electrochem Sci 6:4164–4175

Khalilzadeh B, Shadjou N, Eskandani M, Charoudeh HN, Omidi Y, Rashidi M-R (2015) A reliable self-assembled peptide based electrochemical biosensor for detection of caspase 3 activity and apoptosis. RSC Adv 5(72):58316–58326

Khalilzadeh B, Shadjou N, Charoudeh HN, Rashidi M-R (2017) Recent advances in electrochemical and electrochemiluminescence based determination of the activity of caspase-3. Microchim Acta 184(10):3651–3662

Khalilzadeh B, Rashidi M, Soleimanian A, Tajalli H, Kanberoglu GS, Baradaran B, Rashidi M-R (2019) Development of a reliable microRNA based electrochemical genosensor for monitoring of miR-146a, as key regulatory agent of neurodegenerative disease. Int J Biol Macromol 134:695–703

Khataee A, Haddad Irani-Nezhad M, Hassanzadeh J (2018) Improved peroxidase mimetic activity of a mixture of WS(2) nanosheets and silver nanoclusters for chemiluminescent quantification of H(2)O(2) and glucose. Mikrochim Acta 185(3):190. https://doi.org/10.1007/s00604-018-2727-4

Ma PC, Maulik G, Christensen J, Salgia R (2003) c-Met: structure, functions and potential for therapeutic inhibition. Cancer Metastasis Rev 22(4):309–325

Manzoori JL, Amjadi M, Soleymani J, Tamizi E, Rezamand A, Jouyban A (2012) Determination of deferiprone in urine and serum using a terbium-sensitized luminescence method. Luminescence 27(4):268–273

Massoumi B, Abbasian M, Jahanban-Esfahlan R, Mohammad-Rezaei R, Khalilzadeh B, Samadian H, Rezaei A, Derakhshankhah H, Jaymand M (2020) A novel bio-inspired conductive, biocompatible, and adhesive terpolymer based on polyaniline, polydopamine, and polylactide as scaffolding biomaterial for tissue engineering application. Int J Biol Macromol 147:1174–1184

Polyakov AY, Yadgarov L, Popovitz-Biro R, Lebedev VA, Pinkas I, Rosentsveig R, Feldman Y, Goldt AE, Goodilin EA, Tenne R (2014) Decoration of WS2 nanotubes and fullerene-like MoS2 with gold nanoparticles. J Phys Chem C 118(4):2161–2169

Pourakbari R, Shadjou N, Yousefi H, Isildak I, Yousefi M, Rashidi M-R, Khalilzadeh B (2019) Recent progress in nanomaterial-based electrochemical biosensors for pathogenic bacteria. Microchim Acta 186(12):820

Pourakbari R, Mousavishenas MH, Kamrani A, Dolati S, Abbaszadeh H, Zamani M, Yaghoubi Y, Hashemzadeh S, Ahmadi M, Hojjat-Farsangi M (2020) Identification of genes and miRNAs associated with angiogenesis, metastasis, and apoptosis in colorectal cancer. Gene Rep 18:100552

Pumera M, Loo AH (2014) Layered transition-metal dichalcogenides (MoS2 and WS2) for sensing and biosensing. TrAC Trends Anal Chem 61:49–53

Qamsari ES, Sharifzadeh Z, Bagheri S, Riazi-Rad F, Younesi V, Abolhassani M, Ghaderi SS, Baradaran B, Somi MH, Yousefi M (2017) Isolation and characterization of anti c-met single chain fragment variable (scFv) antibodies. J Immunotoxicol 14(1):23–30

Rathore S, Hussain M, Ali A, Khan A (2013) A recent survey on colon cancer detection techniques. IEEE ACM Trans Comput Biol Bioinf 10(3):545–563

Safaie Qamsari E, Safaei Ghaderi S, Zarei B, Dorostkar R, Bagheri S, Jadidi-Niaragh F, Somi MH, Yousefi M (2017) The c-Met receptor: implication for targeted therapies in colorectal cancer. Tumor Biol 39(5):1010428317699118

Saghatforoush L, Hasanzadeh M, Karim-Nezhad G, Ershad S, Shadjou N, Khalilzadeh B, Hajjizadeh M (2009) Kinetic study of the electrooxidation of mefenamic acid and indomethacin catalysed on cobalt hydroxide modified glassy carbon electrode. Bull Korean Chem Soc 30(6):1341–1348

Saptarshi SR, Duschl A, Lopata AL (2013) Interaction of nanoparticles with proteins: relation to bio-reactivity of the nanoparticle. J Nanobiotechnol 11:26. https://doi.org/10.1186/1477-3155-11-26

Singh A, Glass N, Tolba M, Brovko L, Griffiths M, Evoy S (2009) Immobilization of bacteriophages on gold surfaces for the specific capture of pathogens. Biosens Bioelectron 24(12):3645–3651

Soleymani L, Li F (2017) Mechanistic challenges and advantages of biosensor miniaturization into the nanoscale. ACS Sensors 2(4):458–467

Soleymani J, Perez-Guaita D, Hasanzadeh M, Shadjou N, Jouyban A (2017) Materials and methods of signal enhancement for spectroscopic whole blood analysis: novel research overview. TrAC Trends Anal Chem 86:122–142

Tonelli D, Scavetta E, Gualandi I (2019) Electrochemical deposition of nanomaterials for electrochemical sensing. Sensors 19(5):1186

Wang Z, Zhang J, Guo Y, Wu X, Yang W, Xu L, Chen J, Fu F (2013) A novel electrically magnetic-controllable electrochemical biosensor for the ultra sensitive and specific detection of attomolar level oral cancer-related microRNA. Biosens Bioelectron 45:108–113

Winawer SJ, Flehinger BJ, Schottenfeld D, Miller DG (1993) Screening for colorectal cancer with fecal occult blood testing and sigmoidoscopy. JNCI 85(16):1311–1318

Xu S, Li D, Wu P (2015) One-pot, facile, and versatile synthesis of monolayer MoS2/WS2 quantum dots as bioimaging probes and efficient electrocatalysts for hydrogen evolution reaction. Adv Func Mater 25(7):1127–1136

Xu Y, Yan L, Li X, Xu H (2019) Fabrication of transition metal dichalcogenides quantum dots based on femtosecond laser ablation. Sci Rep 9(1):1–9