Early exercise advances the maturation of glycosaminoglycans and collagen in the extracellular matrix of articular cartilage in the horse

Equine Veterinary Journal - Tập 40 Số 2 - Trang 128-135 - 2008
P. René van Weeren1, Elwyn C. Firth2, Harold Brommer3, Marko Hyttinen4, Heikki J. Helminen4, Chris W. Rogers2, Jeroen DeGroot5, Pieter A.J. Brama6
1Global Equine Research Alliance/Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, NL-3584 CM Utrecht, The Netherlands.
2Global Equine Research Alliance/Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand
3Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
4Institute of Biomedicine, Department of Anatomy, University of Kuopio, Kuopio, Finland
5Business Unit Biosciences, TNO Quality of Life, Leiden, The Netherlands
6Department of Surgery, University College Dublin, Dublin - Ireland

Tóm tắt

Summary

Reasons for performing study: Training at a very young age may influence the characteristics of the collagen network of articular cartilage extracellular matrix (ECM) in horses.

Objectives: To investigate whether increasing workload of foals results in significant changes in the biochemical composition of articular cartilage ECM.

Methods: Thoroughbred foals (n = 33) were divided into 2 different exercise groups from age 10 days‐18 months. One group (PASTEX; n = 15) was reared at pasture; the other (CONDEX; n = 18) underwent a specific additional training programme that increased workload by 30%. At mean age 18 months, 6 animals from each group were subjected to euthanasia. The proximal articular surface of the proximal phalanx of the right hindlimb was examined for the presence of damage using the cartilage degeneration index (CDI). Samples were taken from 2 sites with known different loading patterns. Slices were analysed for DNA, glycosaminoglycans (GAG), collagen and post translational modifications of collagen (formation of hydroxylysylpyridinoline [HP] and pentosidine crosslinks, and hydroxylysine [Hyl]), and exercise groups and different sites compared.

Results: There were no differences in CDI betweenPASTEXandCONDEXanimals, indicating the absence of extra joint damage due to the exercise regimen. There were site‐related differences for most biochemical variables, corroborating earlier reports. All biochemical variables showed differences betweenPASTEXandCONDEXgroups at one of the sites, and some at both. GAG and collagen levels were lower in theCONDEXgroup whereas Hyl, HP crosslinks and pentosidine crosslinks were higher.

Conclusions and potential relevance: A measurable effect of the conditioning exercise was demonstrated. The margin between too much and too little work when training foals may be narrower than intuitively presumed.

Từ khóa


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Equine Veterinary Journal

Tập 40 Số 2

128-135

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