Những biến đổi biểu hiện gen động trong phản ứng với vi chất dinh dưỡng, đại chất dinh dưỡng và nhiều tác động stress ở đậu tương

Springer Science and Business Media LLC - Tập 20 - Trang 321-341 - 2019
Jamie A. O’Rourke1,2, Chantal E. McCabe1, Michelle A. Graham1,2
1Corn Insects and Crop Genetics Research Unit, USDA-ARS, Ames, USA
2Department of Agronomy, Iowa State University, Ames, USA

Tóm tắt

Việc bảo tồn năng suất cây trồng là vô cùng quan trọng đối với sản xuất đậu tương ở Mỹ và nền kinh tế toàn cầu. Các loài cây đã được chọn giống để tăng năng suất trong suốt hàng nghìn năm, với từng dòng được chọn để cải thiện hiệu suất trong các môi trường đặc thù, những hạn chế mà các loài mô hình như Arabidopsis không gặp phải. Quá trình lựa chọn này có thể đã dẫn đến các thích ứng với stress mới lạ, đặc trưng cho các loài cây trồng. Trong bối cảnh thiếu sắt là một vấn đề thường xuyên ở các vùng trồng đậu tương tại Hoa Kỳ và sự thiếu hụt phosphate đang là một hạn chế đối với sản xuất nông nghiệp toàn cầu, các nghiên cứu về stress dinh dưỡng ở các loài cây trồng là vô cùng quan trọng. Trong nghiên cứu này, chúng tôi đã so sánh trực tiếp phản ứng biểu hiện toàn bộ gen của lá và rễ đối với sự thiếu sắt (Fe) và phosphate (Pi), đại diện cho một vi chất dinh dưỡng và một đại chất dinh dưỡng, tương ứng. Thực hiện các thí nghiệm song song, chúng tôi quan sát thấy đậu tương phản ứng với cả hai sự thiếu hụt dinh dưỡng trong vòng 24 giờ. Mặc dù đậu tương chủ yếu phản ứng với sự thiếu hụt –Fe, nhưng nó lại phản ứng rất mạnh với việc cung cấp lại Pi. Mặc dù thời gian phản ứng khác nhau, nhưng cả hai tín hiệu stress dinh dưỡng đều sử dụng những con đường phân tử giống nhau. Nghiên cứu của chúng tôi là nghiên cứu đầu tiên chứng minh tốc độ và sự đa dạng của phản ứng stress ở đậu tương đối với nhiều sự thiếu hụt dinh dưỡng. Chúng tôi cũng đã thiết kế nghiên cứu để xem xét sự thay đổi về biểu hiện gen trong phản ứng với nhiều sự kiện stress. Chúng tôi đã xác định được 865 và 3375 gen có sự thay đổi trong hướng biểu hiện sau lần tiếp xúc stress thứ hai hoặc chỉ có sự biểu hiện khác biệt sau sự kiện stress thứ hai. Việc hiểu biết về các cơ chế phân tử của những phản ứng này ở các loài cây trồng có thể có những tác động lớn đối với việc cải thiện khả năng chịu đựng stress và bảo tồn năng suất.

Từ khóa

#đậu tương #biểu hiện gen #dinh dưỡng #stress dinh dưỡng #thiếu hụt sắt #thiếu hụt phosphate #phản ứng stress

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