Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2

Alessi, 1996, Mechanism of activation of protein kinase B by insulin and IGF-1, EMBO J., 15, 6541, 10.1002/j.1460-2075.1996.tb01045.x Bar-Peled, 2012, SnapShot: mTORC1 signaling at the lysosomal surface, Cell, 151, 1390, 10.1016/j.cell.2012.11.038 Ben-Hur, 2008, Support vector machines and kernels for computational biology, PLoS Comput. Biol., 4, e1000173, 10.1371/journal.pcbi.1000173 Blethrow, 2008, Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates, Proc. Natl. Acad. Sci. USA, 105, 1442, 10.1073/pnas.0708966105 Bridges, 2012, Phosphatidylinositol 3,5-bisphosphate plays a role in the activation and subcellular localization of mechanistic target of rapamycin 1, Mol. Biol. Cell, 23, 2955, 10.1091/mbc.e11-12-1034 Chi, 2008, Identification of CDK2 substrates in human cell lysates, Genome Biol., 9, R149, 10.1186/gb-2008-9-10-r149 Colaert, 2009, Improved visualization of protein consensus sequences by iceLogo, Nat. Methods, 6, 786, 10.1038/nmeth1109-786 Cox, 2008, MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification, Nat. Biotechnol., 26, 1367, 10.1038/nbt.1511 Cox, 2011, Andromeda: a peptide search engine integrated into the MaxQuant environment, J. Proteome Res., 10, 1794, 10.1021/pr101065j Facchinetti, 2008, The mammalian target of rapamycin complex 2 controls folding and stability of Akt and protein kinase C, EMBO J., 27, 1932, 10.1038/emboj.2008.120 Frias, 2006, mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms define three distinct mTORC2s, Curr. Biol., 16, 1865, 10.1016/j.cub.2006.08.001 Futschik, 2005, Noise-robust soft clustering of gene expression time-course data, J. Bioinform. Comput. Biol., 3, 965, 10.1142/S0219720005001375 Gu, 2000, ei24, a p53 response gene involved in growth suppression and apoptosis, Mol. Cell. Biol., 20, 233, 10.1128/MCB.20.1.233-241.2000 Hsu, 2011, The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling, Science, 332, 1317, 10.1126/science.1199498 Huttlin, 2010, A tissue-specific atlas of mouse protein phosphorylation and expression, Cell, 143, 1174, 10.1016/j.cell.2010.12.001 Jacinto, 2006, SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity, Cell, 127, 125, 10.1016/j.cell.2006.08.033 Laplante, 2012, mTOR signaling in growth control and disease, Cell, 149, 274, 10.1016/j.cell.2012.03.017 Larance, 2010, Global phosphoproteomics identifies a major role for AKT and 14-3-3 in regulating EDC3, Mol. Cell. Proteomics, 9, 682, 10.1074/mcp.M900435-MCP200 Larsen, 2005, Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns, Mol. Cell. Proteomics, 4, 873, 10.1074/mcp.T500007-MCP200 Linding, 2008, NetworKIN: a resource for exploring cellular phosphorylation networks, Nucleic Acids Res., 36, D695 Manning, 2007, AKT/PKB signaling: navigating downstream, Cell, 129, 1261, 10.1016/j.cell.2007.06.009 Michalski, 2011, Mass spectrometry-based proteomics using Q Exactive, a high-performance benchtop quadrupole Orbitrap mass spectrometer, Mol. Cell. Proteomics, 10, 10.1074/mcp.M111.011015 Obata, 2000, Peptide and protein library screening defines optimal substrate motifs for AKT/PKB, J. Biol. Chem., 275, 36108, 10.1074/jbc.M005497200 Obenauer, 2003, Scansite 2.0: Proteome-wide prediction of cell signaling interactions using short sequence motifs, Nucleic Acids Res., 31, 3635, 10.1093/nar/gkg584 Olsen, 2006, Global, in vivo, and site-specific phosphorylation dynamics in signaling networks, Cell, 127, 635, 10.1016/j.cell.2006.09.026 Ong, 2006, A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC), Nat. Protoc., 1, 2650, 10.1038/nprot.2006.427 Pan, 2012, Structures of the pleckstrin homology domain of Saccharomyces cerevisiae Avo1 and its human orthologue Sin1, an essential subunit of TOR complex 2, Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 68, 386, 10.1107/S1744309112007178 Sarbassov, 2005, Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex, Science, 307, 1098, 10.1126/science.1106148 Schmidlin, 2004, The ARE-dependent mRNA-destabilizing activity of BRF1 is regulated by protein kinase B, EMBO J., 23, 4760, 10.1038/sj.emboj.7600477 Schroder, 2007, Human Sin1 contains Ras-binding and pleckstrin homology domains and suppresses Ras signalling, Cell. Signal., 19, 1279, 10.1016/j.cellsig.2007.01.013 Schwanhäusser, 2011, Global quantification of mammalian gene expression control, Nature, 473, 337, 10.1038/nature10098 Wiśniewski, 2009, Combination of FASP and StageTip-based fractionation allows in-depth analysis of the hippocampal membrane proteome, J. Proteome Res., 8, 5674, 10.1021/pr900748n Yu, 2011, Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling, Science, 332, 1322, 10.1126/science.1199484 Yuan, 2008, PI3K pathway alterations in cancer: variations on a theme, Oncogene, 27, 5497, 10.1038/onc.2008.245 Zoncu, 2011, mTOR: from growth signal integration to cancer, diabetes and ageing, Nat. Rev. Mol. Cell Biol., 12, 21, 10.1038/nrm3025