Dual recognition of S 1 and S 4 pistils by S 4 sm pollen in self-incompatibility of Japanese pear (Pyrus pyrifolia Nakai)
Tóm tắt
Most cultivars of Japanese pear (Pyrus pyrifolia Nakai) exhibit gametophytic self-incompatibility controlled by a single S-locus with multiple S-haplotypes. A self-compatible (SC) cultivar, ‘Osanijisseiki’ (S
2
S
4
sm
), arising by a bud mutation of ‘Nijisseiki’ (S
2
S
4
), has a stylar-part mutant S
4
sm
-haplotype, which lacks the pistil S
4
gene, which is the S
4
-RNase gene. To efficiently breed SC cultivars, we selected ‘Nashi Chuukanbohon Nou 1 Gou’ (‘NCN1’) harboring homozygous S
4
sm
from a self-progeny of Osanijisseiki and crossed it with ‘Okusankichi’ (S
5
S
7
), ‘Hakkou’ (S
4
S
5
), or ‘Ri-14’ (S
1
S
2
). Fruit set (%) was compared after self-pollination of the trees in the three progenies. All trees derived from the three progenies were predicted to be SC, except for the S
4
S
4
sm
trees in the progeny of NCN1 × Hakkou. However, S
1
S
4
sm
trees in the progeny of NCN1 × Ri-14 proved to be self-incompatible (SI). The pollen from Osanijisseiki was incompatible with ‘Doitsu’ (S
1
S
2
), but that from Nijisseiki was compatible, suggesting a possibility that the S
4
sm
pollen was rejected by S
1
-harboring pistils. This possibility was clarified by crossing the pollen from NCN1 (S
4
sm
S
4
sm
) to Doitsu, ‘Imamuraaki’ (S
1
S
6
), or ‘Hougetsu’ (S
1
S
7
), all of which proved incompatible. On the other hand, S
4
sm
pollen was accepted by pistils harboring the S
2
, S
3
, S
5
, S
6
, S
7
, S
9
, and S
k
haplotypes. The dual recognition of S
1
and S
4
pistils by S
4
sm
pollen can be attributed to a mutation of the pollen S
4
gene(s).
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