Dual effect of blue light on Fusariumsolani clinical corneal isolates in vitro
Tóm tắt
The purpose was to investigate the effect of daylight-intensity blue light on F. solani isolated from the cornea of patients with fungal keratitis. Spore suspensions of 5 F. solani strains (one standard strain and 4 clinical corneal isolates) were prepared in 6-well plates. Blue light groups were irradiated by a light-emitting diode (LED) device with a peak wavelength of 454 nm at 0.5 mW/cm2 for 0 to 48 h, while the controls were maintained in darkness. Hyphal morphology in the 6-well plates was recorded at 0, 12, 24, 36, 48 h. One hundred microliters of spore suspensions of each strain at these five time points was transferred to SGA plates and cultured for 36 h at 29 °C; the number of colonies formed was counted as a measure of conidia quality and viability. Blue light has dual effects on F. solani. The hyphal length of F. solani exposed to blue light was significantly shorter than that of the control (P < 0.01), indicating that fungal growth was inhibited. Meanwhile, instead of reducing the viability of spores, blue light significantly enhanced the conidia quality and viability after at least 24 h irradiation. Daylight-intensity blue light exposure will inhibit the hyphal growth of F. solani but promote conidiation, which would be more harmful to fungal keratitis. Eliminating the influence of blue light for these patients should be taken into account.
Tài liệu tham khảo
Garg P, Gopinathan U, Choudhary K et al (2000) Keratomycosis: clinical and microbiologic experience with dematiaceous fungi. Ophthalmology 107:574–580
Maharana PK, Sharma N, Nagpal R, Jhanji V, Das S, Vajpayee RB (2016) Recent advances in diagnosis and management of mycotic keratitis. Indian J Ophthalmol 64(5):346–357
Srinivasan M (2004) Fungal keratitis. Curr Opin Ophthalmol 15:321–327
Tupaki-Sreepurna A, Al-Hatmi AMS, Kindo AJ, Sundaram M, de Hoog GS (2017) Multidrug-resistant Fusarium in keratitis: a clinic-mycological study of keratitis infections in Chennai, India. Mycoses 60:230–233
Rose-Nussbaumer J, Venkatesh Prajna N, Tiruvengada Krishnan K, Mascaren-has J, Rajaraman R, Srinivasan M et al (2015) Vision-related quality-of-life outcomes in the mycotic ulcer treatment trial I: a randomized clinical trial. JAMA Ophthalmol 133:642–646
Thomas PA, Kaliamurthy J (2013) Mycotic keratitis: epidemiology, diagnosis and management. Clin Microbiol Infect 19(3):210–220
Oechsler RA, Feilmeier MR, Ledee D et al (2009) Utility of molecular sequence analysis of the ITS rRNA region for identification of Fusarium spp from ocular sources. Invest Ophthalmol Vis Sci 50:2230–2236
O’Donnell K, Sarver BA, Brandt M et al (2007) Phylogenetic diversity and microsphere array-based genotyping of human pathogenic Fusaria, including isolates from the multistate contact lens-associated U.S. keratitisoutbreaks of 2005 and 2006. J Clin Microbiol 45:2235–2248
Herkert PF, Al-Hatmi AMS, de Oliveira Salvador GL, Muro MD, Pinheiro RL, Nucci M, Queiroz-Telles F, de Hoog GS, Meis JF (2019) Molecular characterization and antifungal susceptibility of clinical Fusarium species from Brazil. Front Microbiol 10:737
Tupaki-Sreepurna A, Al-Hatmi AM, Kindo AJ, Sundaram M, de Hoog GS (2017) Multidrug-resistant Fusarium in keratitis: a clinico-mycological study of keratitis infections in Chennai. India Mycoses 60:230–233
Coleman JJ (2016) The Fusarium solani species complex: ubiquitous pathogens of agricultural importance. Mol Plant Pathol 17:146–158
Tomb RM, White TA, Coia JE, Anderson JG, MacGregor SJ, Maclean M (2018) Review of the Comparative Susceptibility of Microbial Species to Photoinactivation Using 380-480 nm Violet-Blue Light. Photochem Photobiol 94(3):445–458.
Casas-Flores S, Rios-Momberg M, Rosales-Saavedra T, Martínez-Hernández P, Olmedo-Monfil V, Herrera-Estrella A (2006) Cross-talk between a fungal blue-light perception system and the cyclic AMP signaling pathway. Eukaryot Cell 5:499–506
Purschwitz J, Müller S, Kastner C, Fischer R (2006) Seeing the rainbow: light sensing in fungi. Curr Opin Microbiol 9:566–571
Fanelli F, Schmidt-Heydt M, Haidukowski M, Susca A, Geisen R, Logrieco A, Mulè G (2012) Influence of light on growth, conidiation and fumonisin production by Fusarium verticillioides. Fungal Biol 116:241–248
Fanelli F, Schmidt-Heydt M, Haidukowski M, Geisen R, Logrieco A, Mulè G (2012) Influence of light on growth, fumonisin biosynthesis and FUM1 gene expression by Fusarium proliferatum. Int J Food Microbiol 153:148–153
De Lucca AJ, Carter-Wientjes C, Williams KA, Bhatnagar D (2012) Blue light (470 nm) effectively inhibits bacterial and fungal growth. Lett Appl Microbiol 55(6):460–466
Trzaska WJ, Wrigley HE, Thwaite JE, May RC (2017) Species-specific antifungal activity of blue light. Sci Rep 7(1):4605
Marek V, Mélik-Parsadaniantz S, Villette T, Montoya F, Baudouin C, Brignole-Baudouin F, Denoyer A (2018) Blue light phototoxicity toward human corneal and conjunctival epithelial cells in basal and hyperosmolar conditions. Free Radic Biol Med 126:27–40
Núñez-Álvarez C, Osborne NN (2019) Enhancement of corneal epithelium cell survival, proliferation and migration by red light: relevance to corneal wound healing. Exp Eye Res 180:231–241
Anutarapongpan O, Maestre-Mesa J, Alfonso EC, O’Brien TP, Miller D (2018) Multiplex polymerase chain reaction assay for screening of mycotoxin genes from ocular isolates of Fusarium species. Cornea 37(8):1042–1046
Hazlett L, Suvas S, McClellan S, Ekanayaka S (2016) Challenges of corneal infections. Expert Rev Ophthalmol 11:285–297
Liang YI, Lu LM, Chen Y, Lin YK (2016) Photodynamic therapy as an antifungal treatment. Exp Ther Med 12:23–27
Lembo AJ, Ganz RA, Sheth S, Cave D, Kelly C, Levin P, Kazlas PT, Baldwin PC 3rd, Lindmark WR, McGrath JR, Hamblin MR (2009) Treatment of Helicobacter pylori infection with intragastric violet light phototherapy: a pilot clinical trial. Lasers Surg Med 41:337–344
Zhang Y, Zhu Y, Gupta A, Huang Y, Murray CK, Vrahas MS, Sherwood ME, Baer DG, Hamblin MR, Dai T (2014) Antimicrobial blue light therapy for multidrug-resistant Acinetobacter baumannii infection in a mouse burn model: implications for prophylaxis and treatment of combat-related wound infections. J Infect Dis 209:1963–1971
Zhang Y, Zhu Y, Chen J, Wang Y, Sherwood ME, Murray CK, Vrahas MS, Hooper DC, Hamblin MR, Dai T (2016) Antimicrobial blue light inactivation of Candida albicans: In vitro and in vivo studies. Virulence 7:536–545
Lee HS, Cui L, Li Y et al (2016) Influence of light emitting diode-derived blue light overexposure on mouse ocular surface. PLoS One 11(8):e0161041
Behar-Cohen F, Martinsons C, Viénot F et al (2011) Light-emitting diodes (LED) for domestic lighting: any risks for the eye? Prog Retin Eye Res 30(4):239–257
Huang C, Zhang P, Wang W, Xu Y, Wang M, Chen X, Dong X (2014) Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways. Apoptosis 19(6):922–932
Tan KK (1976)Light-induced synchronous conidiation in the fungus Botrytis cinerea. J Gen Microbiol 93(2):278–282
Kumagai T (1989) Temperature and mycochrome system in near-UV light inducible and blue light reversible photoinduction of conidiation Alternaria tomato. Photochem Photobiol Sci 50:793e798
Zhu JC, Wang XJ, Zhang G, Su J, Zhu M (2006) Glucoamylase enhancement regulated by blue light in Aspergillus niger. Wei Sheng Wu Xue Bao 46(5):734–739
Kiryu H, Yoshida S, Suenaga Y, Asahi M (1991) Invasion and survival of Fusarium solani in the dexamethasone-treated cornea of rabbits. J Med Vet Mycol 29(6):395–406
Robertson MD, Seaton A, Milne LJ, Raeburn JA (1987) Resistance of spores of Aspergillus fumigatus to ingestion by phagocytic cells. Thorax 42(6):466–472
Choy CK, Benzie IF, Cho P (2005)UV-mediated DNA strand breaks in corneal epithelial cells assessed using the comet assay procedure. Photochem Photobiol 81(3):493–497
Sliney DH (2006) Risks of occupational exposure to optical radiation. Med Lav 97(2):215–220
Stamatacos C, Harrison JL (2014) The possible ocular hazards of LED dental illumination applications. J Mich Dent Assoc 96(4):34–39
Moorhead S, Maclean M, MacGregor SJ, Anderson JG (2016) Comparative sensitivity of Trichophyton and Aspergillus conidia to inactivation by violet-blue light exposure. Photomed Laser Surg 34:36–41
Lee HS, Cui L, Li Y, Choi JS, Choi JH, Li ZR, Kim GE, Choi W, Yoon KC (2016) Correction: influence of light emitting diode-derived blue light overexposure on mouse ocular surface. PLoS One 11(11):e0167671
Zhao ZC, Zhou Y, Tan G, Li J (2018) Research progress about the effect and prevention of blue light on eyes. Int J Ophthalmol 11(12):1999–2003