Does abscisic acid and xylem sap pH regulate stomatal responses in papaya plants submitted to partial root-zone drying?
Tóm tắt
Papaya (Carica papaya L.) is a fruit plant that requires high water availability, and therefore irrigation is required throughout its cropping cycle. Some irrigation techniques can be used to improve water use efficiency; Partial root-zone drying fixed (PRDFixed) is one of these techniques where water is applied only to one side of the root. Thus, water applied to one side of the root maintains leaf water status and plant growth, and uses root signaling (abscisic acid, ABA) to reduce stomatal conductance and transpiration, without affecting CO2 assimilation. Here we checked whether papaya plants submitted to PRDFixed technique changes shoot ABA and proline concentrations, and if these responses would be associated to alteration in xylem sap pH, gas exchange rates and plant growth. The root system of young papaya plants were divided into two square pots and three treatments: fully irrigated (FI) (soil kept at field capacity in both pots), DS (drought stress, irrigation was suspended in both pots) and PRDFixed (irrigation was suspended in one pot and in the other pot the soil was kept at field capacity). No difference was observed in proline concentration between fully-irrigated and PRDFixed plants. Drying one side of the root system was not sufficient to promote alkalinization of the xylem sap pH or consistently stimulate foliar ABA accumulation. Although higher rates of CO2 assimilation and transpiration were noted in PRDFixed plants compared to drought-stressed plants, this decrease would continue if water limitation was longer than 11 days. Consequently, this decrease would affect biomass over time. Thus, in the first 11 days of water limitation, the stomatal closure of PRDFixed plants was not affected either by increase in ABA or by alkalinization of the xylem sap.
Tài liệu tham khảo
Abboud S, Dbara A, Abidi W, Braham M (2019) Differential agro physiological responses induced by partial root zone drying irrigation in olive cultivars grown in semi arid conditions. Environ Exp Bot 167:103863
Abboud S, Vives-Peris V, Dbara S, Gomez-Cadenas A, Perez-Clemente RM, Abidi W, Braham M (2021) Water status, biochemical and hormonal changes involved in the response of Olea europaea L. to water deficit induced by partial root-zone drying irrigation (PRD). Sci Hort 276:1–15. https://doi.org/10.1016/j.scienta.2020.109737
Ahmadi SH, Andersen MN, Plauborg F, Poulsen RT, Jensen CR, Sepaskhah AR, Hansen S (2010) Effects of irrigation strategies and soils on field grown potatoes: yield and water productivity. Agric Water Manag 97:1923–1930
Aryal R, Ming R (2014) Sex determination in flowering plants: papaya as a model plant. Plant Sci 217–217:56–62
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
Batool A, Cheng ZG, Akram NA, Lv GC, Xiong JL, Zhu Y, Ashraf M, Xiong YC (2019) Partial and full root-zone drought stresses account for differentiate root sourced signal and yield formation in primitive wheat. Plant Methods 15(1):75. https://doi.org/10.1186/s13007-019-0461-5
Bolhár-Nordenkampf HR, Long SP, Baker NR, Oquist G, Schreibers U, Lecher EG (1989) Chlorophyll fluorescence as a probe of the photosynthetic competence of leaves in the field: a review of current instrumentation. Funct Ecol 3:497–514. https://doi.org/10.2307/2389624
Campostrini E, Schaffer B, Ramalho JD, González JC, Rodrigues WP, Silva JR, Lima RS (2018) Environmental factors controlling carbon assimilation, growth, and yield of papaya (Carica papaya L.) under water-scarcity scenarios. In: Tejero IFG, Zuazo VHD (eds) Water scarcity and sustainable agriculture in semiarid environment. Academic Press, London. https://doi.org/10.1016/B978-0-12-813164-0.00019-3
Castro FA, Campostrini E, Torres Netto A, Gomes MMA, Ferraz TM, Glenn DM (2014) Portable chlorophyll meter (PCM-502) values are related to total chlorophyll concentration and photosynthetic capacity in papaya (Carica papaya L). Theor Exp Plant Physiol 26:201–210
Dbara S, Haworth M, Emiliani G, Ben Mimoun M, Gómez-Cadenas A, Centritto M (2016) Partial root-zone drying of olive (Olea europaea var. ‘Chetoui’) induces reduced yield under field conditions. PLoS ONE 11(6):e0157089. https://doi.org/10.1371/journal.pone.0157089
Dodd IC, Egea G, Davies WJ (2008) Abscisic acid signaling when soil moisture is heterogeneous: decreased photoperiod sap flow from drying roots limits abscisic acid export to the shoots. Plant Cell Environ 31(9):1263–1274. https://doi.org/10.1111/j.1365-3040.2008.01831.x
Du S, Kang S, Li F, Du T (2017) Water use efficiency is improved by alternate partial root-zone irrigation of apple in arid northwest. Agric Water Manag 179:184–192. https://doi.org/10.1016/j.agwat.2016.05.011
Espadas JL, Castaño E, Marina ML, Rodríguez LC, Plaza M (2019) Phenolic compounds increase their concentration in Carica papaya leaves under drought stress. Acta Physiol Plant 41:180. https://doi.org/10.1007/s11738-019-2972-0
Fernández JE, Alcon F, Diaz-Espejo A, Hernandez-Santana V, Cuevas MV (2020) Water use indicators and economic analysis for on-farm irrigation decision: a case study of a super high density olive tree orchard. Agric Water Manag 106074. https://doi.org/10.1016/j.agwat.2020.106074
Geiger D, Maierhofer T, Al-Rasheid KA, Scherzer S, Mumm P, Liese A, Ache P, Wellmann C, Marten I, Grill E, Romeis T, Hedrich R (2011) Stomatal closure by fast abscisic acid signaling is mediated by the guard cell anion channel SLAH3 and the receptor RCAR1. Sci Signal Signal 4:ra32
Ghafari H, Hassanpour H, Jafari M, Besharat S (2020) Physiological, biochemical and gene-expressional responses to water deficit in apple subjected to partial root-zone drying (PRD). Plant Physiol Biochem 148:333–346
Gomes MMA, Netto AT, Campostrini E, Bressan-Smith R, Zullo MAT, Ferraz TM, Siqueira LD, Leal NR, Núñez-Vázquez M (2013) Brassinosteroid analogue affects the senescence in two papaya genotypes submitted to drought stress. Theor Exp Plant Physiol 25:186–195
Han J, Lei Z, Zhang Y, Yi X, Zhang W (2019) Drought introduced variability of mesophyll conductance in Gossypium and its relationship with leaf anatomy. Physiol Plant 166:873–887
Iqbal R, Raza MAS, Toleikiene M, Ayas M, Hashemi F, Habib-ur-Rahman M, Zaheer MS, Ahmad S, Riaz U, Ali M, Aslam MU, Haider I (2020) Partial root-zone drying (PRD), its effects and agricultural significance: a review. Bull Natl Res Center 44:159. https://doi.org/10.1186/s42269-020-00413-w
Jovanovic Z, Stikic R (2018) Partial Root-Zone drying technique: from Water saving to the improvement of a Fruit Quality. Front Sustainable Food Syst. https://doi.org/10.3389/fsufs.2017.00003
Kempe A, Lautenschläger T, Lange A, Neinhuis C (2014) How to become a tree without wood - biomechanical analysis of the stem of Carica papaya L. Plant Biol 16:264–271
Khapte PS, Kumar P, Burman U, Kumar P (2019) Deficit irrigation in tomato: agronomical and physio-biochemical implications. Sci Hort 248:256–264
Kumar P, Rouphael Y, Cardarelli M, Colla G (2017) Vegetable grafting as a tool to improve drought resistance and water use efficiency. Front Plant Sci 8:1130. https://doi.org/10.3389/fpls.2017.01130
Lamaoui M, Chakhchar A, Kharrassi EL, Wahbi Y, El Modafar S C (2018) Morphological, physiological, and biochemical responses to water stress in melon (Cucumis melo) subjected to regulated deficit irrigation (RDI) and partial root zone drying (PRD). J Crop Sci Biotechnol 21(4):407–416. https://doi.org/10.1007/s12892-018-0122-0
Li W, Jia L, Wang L (2017) Chemical signals and their regulations on the plant growth and water use efficiency of cotton seedlings under partial root-zone drying and different nitrogen applications. Saudi J Biol Sci 24(3):477–487. https://doi.org/10.1016/j.sjbs.2017.01.015
Li X, Kang S, Zhang X, Li F, Lu H (2018) Deficit irrigation provokes more pronounced responses of maize photosynthesis and water productivity to elevated CO2. Agric Water Manag 195:71–83. https://doi.org/10.1016/j.agwat.2017.09.017
Lima RSN, Figueiredo FAMMA, Oliveira AM, Deus BCS, Ferraz TM, Gomes MMA, Sousa EF, Glenn DM, Campostrini E (2015) Partial rootzone drying (PRD) and regulated deficit irrigation (RDI)effects on stomatal conductance, growth, photosynthetic capacity,and water-use efficiency of papaya. Sci Hort 183:13–22
Liu X, Wei Z, Manevski K, Liu J, Ma Y, Andersen MN, Liu F (2021) Partial root-zone drying irrigation increases water-use efficiency of tobacco plants amended with biochar. Ind Crops Prod 166:1–11. https://doi.org/10.1016/j.indcrop.2021.113487
Luu D-T, Maurel C (2005) Aquaporins in a challenging environment: molecular gears for adjusting plant water status. Plant Cell Environ 28:85–96
Mahouachi J, Marrero-Díaz E (2022) Plant growth and fruit nutrient changes in Carica papaya L. genotypes subjected to regulated deficit irrigation. Life 12:1831. https://doi.org/10.3390/life12111831
Mahouachi J, Arbona V, Gómez-Cadenas AG (2007) Hormonal changes in papaya seedlings subjected to progressive water stress and re-watering. Plant Growth Regul 53:43–51
Mattar MA, ElAbedin TKZ, Alazba AA, AlGhobari HM (2020) Soil water status and growth of tomato with partial rootzone drying and deficit drip irrigation techniques. Irrig Sci 38:163–176. https://doi.org/10.1007/s00271-019-00658-y
Maurel C, Simonneau T, Sutka M (2010) The significance of roots as hydraulic rheostats. J Exp Bot 61:3191–3198
McLean EH, Ludwig M, Grierson PF (2011) Root hydraulic conductance and aquaporin abundance respond rapidly to partial root-zone drying events in a riparian Melaleuca species. New Phytol 192:664–675
Ming R, Yu Q, Blas A, Chen C, Na JK, Moore PH (2008) Genomics of papaya, a common source of vitamins in the tropics. In: Moore PH, Ming R (eds) Genomics of tropical crop plants, vol 1. Springer, New York, pp 405–420
Miranda MT, Silva SF, Moura BB, Hayashi AH, Machado EC, Ribeiro RV (2018) Hydraulic redistribution in Citrus rootstocks under drought. Theor Exp Plant Physiol 30:165–172
Nasrabadi M, Ramezanian A, Eshghi S, Kamgar-Haghighi AA, Vazifeshenas MR, Valero MR D (2019) Biochemical changes and winter hardiness in pomegranate (Punica granatum L.) trees grown under deficit irrigation. Sci Hort 251:39–47
Norman SM, Maier VP, Pon DL (1990) Abscisic acid accumulation and carotenoid and chlorophyll content in relation to water stress and leaf age of different types of citrus. J Agric Food Chem 38:1326–1334
Parry MAJ, Androlojc PJ, Khan S, Lea PJ, Keys AJ (2002) Rubisco activity: effects of drought stress. Ann Bot 89:833–839
Pérez-Pérez JG, Navarro JM, Robles JM, Dodd IC (2018) Prolonged drying cycles stimulate ABA accumulation in Citrus macrophylla seedlings exposed to partial rootzone drying. Agric Water Manag 210:271–278. https://doi.org/10.1016/j.agwat.2018.08.020
Puértolas J, Dodd IC (2022) Evaluating soil evaporation and transpiration responses to alternate partial rootzone drying to minimise water losses. Plant Soil 480:473–489. https://doi.org/10.1007/s11104-022-05594-z
Raza MAS, Ahmad S, Saleem MF, Khan IH, Iqbal R, Zaheer MS, Haider I, Ali M (2017) Physiological and biochemical assisted screening of wheat varieties under partial rhizosphere drying. Plant Physiol Biochem 116:150–166
Santos DL, Coelho EF, Cunha FF, Donato SLR, Bernado WP, Rodrigues WP, Campostrini E (2021) Partial root-zone drying in field-grown papaya: gas exchange, yield, and water use efficiency. Agric Water Manag 243:106421. https://doi.org/10.1016/j.agwat.2020.106421
Shrestha A, Cudjoe DK, Kamruzzaman M, Siddique S, Fiorani F, Léon J, Naz AA (2021) Abscisic acid-responsive element binding transcription factors contribute to proline synthesis and stress adaptation in Arabidopsis. J Plant Physiol 261:153414. https://doi.org/10.1016/j.jplph.2021.153414
Wan X, Steudle E, Hartung W (2004) Gating of water channels (aquaporins) in cortical cells of young corn roots by mechanical stimuli (pressure pulses): effects of ABA and of HgCl2. J Exp Bot 55:411–422
Wang Y, Liu F, Jensen CR (2012) Comparative effects of deficit irrigation and alternate partial root-zone irrigation on xylem pH, ABA and ionic concentrations in tomatoes. J Exp Bot 63(5):1907–1917. https://doi.org/10.1093/jxb/err370
Yao C, Moreshet S, Aloni B (2001) Water relations and hydraulic control of stomatal behaviour in bell pepper plant in partial soil drying. Plant Cell Environ 24(2):227–235