Divergent and reticulate species relationships in Leucaena (Fabaceae) inferred from multiple data sources: insights into polyploid origins and nrDNA polymorphism

American Journal of Botany - Tập 89 Số 7 - Trang 1057-1073 - 2002
Colin E. Hughes1, C. Donovan Bailey1, Stephen A. Harris1
1Department of Plant Sciences, University of Oxford, South Parks Rd., Oxford OX1 3RB, UK

Tóm tắt

Previous analyses of species relationships and polyploid origins in the mimosoid legume genus Leucaena have used chloroplast DNA (cpDNA) restriction site data and morphology. Here we present an analysis of a new DNA sequence data set for the nuclear ribosomal DNA (nrDNA) 5.8S subunit and flanking ITS 1 and ITS 2 spacers, a simultaneous analysis of the morphology, ITS and cpDNA data sets for the diploid species, and a detailed comparison of the cpDNA and ITS gene trees, which include multiple accessions of all five tetraploid species. Significant new insights into species relationships and polyploid origins, including that of the economically important tropical forage tree L. leucocephala, are discussed. Heterogeneous ITS copy types, including 26 putative pseudogene sequences, were found within individuals of four of the five tetraploid and one diploid species. Potential pseudogenes were identified using two pairwise comparison approaches as well as a tree‐based method that compares observed and expected proportions of total ITS variation contributed by the 5.8S subunit optimized onto branches of one of the ITS gene trees. Inclusion of putative pseudogene sequences in the analysis provided evidence that some pseudogenes in allopolyploid L. leucocephala are not the result of post‐allopolyploidization gene silencing, but were inherited from its putative diploid maternal progenitor L. pulverulenta.

Từ khóa


Tài liệu tham khảo

10.1139/g97-796

Arnheim N., 1983, Evolution of genes and proteins, 38

10.1093/sysbio/46.4.654

10.2307/2399880

Brewbaker J. L., 1987, Agroforestry: a decade of development, 289

Brewbaker J. L., 1990, Advances in new crops, 283

Brewbaker J. L., 1989, Leucaena Psyllid. Problems and management, 105–110. Proceedings International Workshop

10.1007/BF00942144

Britton N. L., 1928, Mimosaceae, North American Flora, 23, 121

10.1093/oxfordjournals.molbev.a025621

10.1093/oxfordjournals.molbev.a025622

10.1093/genetics/145.3.821

10.1093/sysbio/42.3.384

10.1093/oxfordjournals.molbev.a025705

10.1111/j.1095-8339.2000.tb00550.x

10.1007/BF02861449

Chamberlain J. R., 1993, The taxonomy and population structure of the tropical trees Leucaena and Gliricidia

10.1080/106351598260923

10.1006/mpev.2000.0845

10.1007/s10592-005-9073-x

10.1071/SB9900125

10.1098/rspb.2000.1157

10.2307/2446364

10.1111/j.1096-0031.1994.tb00181.x

10.2307/2412923

Frahm‐Leliveld J. A., 1957, Observations cytologiques sur quelques Leguminoses tropicales et subtropicales, Revue de Cytologie et de Biologie Vegetales, 8, 273

10.1046/j.1365-294X.1999.00690.x

10.1007/BF00985265

10.2307/2656581

Goloboff P.2000.NONA: a tree searching program. Program and documentation:http://www.cladistics.com.

10.2135/cropsci1967.0011183X000700020014x

10.1017/S0960428600000378

10.1007/BF00984639

10.1007/BF00985339

Hartman T. P. V. J.Jones N. W.Blackhall J. B.Power E. C.Cocking andM. R.Davey.2000.Cytogenetics molecular cytogenetics and genome size inLeucaena(Leguminosae Mimosoideae).InH.Guttenberger Ẑ.Borzan S. E.Schlarbaum andT. P. V.Hartman[eds.] Cytogenetic studies of forest trees and shrubs—review present status and outlook for the future 57–70.Forest Genetics. Special Issue.

10.1007/s002390010200

10.1201/9781439833278.ch14

10.1016/0169-5347(96)10006-9

10.2307/4110544

Hughes C. E., 1997, Species delimitation, and new taxa and combinations in Leucaena (Leguminosae), Contributions University of Michigan Herbarium, 21, 277

10.2307/25027876

Hughes C. E., 1998, Leucaena

10.1007/BF00986251

10.1007/BF00985221

Hughes C. E., 1999, Environmental hazards of Leucaena, 61

10.1006/mpev.1997.0465

10.1007/BF00224057

10.2307/2419645

10.1007/s004120050424

10.1007/BF00013763

10.2307/2419452

Luckow M., 2000, Advances in legume systematics, Part 9, 165

Maniatis T., 1982, Molecular cloning: a laboratory manual

10.1111/j.1558-5646.1990.tb03856.x

10.1111/j.1558-5646.1992.tb01127.x

McDade L. A., 1995, Hybridization and phylogenetics, Experimental and molecular approaches to plant biosystematics Monographs in Systematic Botany, 53, 305

Merrill E. D., 1912, Notes on the flora of Manila with special reference to the introduced element, Philippine Journal of Scientific Botany, 7, 145

10.2307/2413255

National Academy of Sciences, 1984, Leucaena: promising forage and tree crop for the tropics

Nixon K. C., 1999, WinClada (Beta)

10.1111/j.1096-0031.1999.tb00277.x

10.1111/j.1096-0031.1996.tb00010.x

10.2307/2419615

10.1073/pnas.78.9.5533

10.1508/cytologia.60.31

10.1508/cytologia.53.393

Pound B., 1983, Leucaena: its cultivation and use

10.1002/j.1537-2197.1995.tb15711.x

10.1080/713608045

10.1093/sysbio/41.1.4

10.1073/pnas.92.15.6813

Schifino‐Wittmann M. T. M.Cardoso T.Boff andC.Simioni.2000.Chromosome numbers and unreduced gametes in species ofLeucaenaBenth. (Leguminosae)—new contributions for the taxonomy evolutionary studies and breeding of the genus.InH.Guttenberger Ẑ.Borzan S. E.Schlarbaum andT. P. V.Hartman[eds.] Cytogenetic studies of forest trees and shrubs—review present status and outlook for the future 181–190.Forest Genetics. Special Issue.

Shibata K., 1962, Estudios citológicos de plantas colombianos silvestres y cultivadas, Journal of Agricultural Science, 8, 49

10.1093/sysbio/46.4.765

10.1093/sysbio/49.2.369

Soltis D. E., 1999, Polyploidy: recurrent formation and genome evolution, Trends in Ecology and Evolution, 14, 349, 10.1016/S0169-5347(99)01638-9

10.1007/978-1-4615-3276-7_8

Sorensson C. T., 1995, Potential for improvement of Leucaena through interspecific hybridization, 47

10.1002/j.1537-2197.1994.tb15435.x

10.1002/j.1537-2197.1993.tb15332.x

10.1007/BF00226978

10.1093/nar/25.24.4876

10.1016/0169-5347(92)90228-4

10.1111/j.1601-5223.1948.tb02831.x

10.1007/BF00985577

10.1093/oxfordjournals.molbev.a026112

10.1139/g96-020

10.1073/pnas.92.1.280

10.1007/BF02861922

White T. J., 1990, PCR protocols: a guide to methods and applications, 315

10.2307/2656776

10.1006/mpev.1999.0648

Zárate S., 1984, Taxonomic revision of the genus Leucaena from Mexico, Bulletin of the International Group for the Study of Mimosoideae, 12, 24

10.5962/bhl.part.14435

Zárate S., 1994, Revisión del género Leucaena Benth. en México, Anales del Instituto de Biología Universidad Nacional Autónoma de México, Botánica, 65, 83

10.1007/BF02862093

Zárate S., 1998, La domesticación de Leucaena en México, Boletín de la Sociedad Botánica de México, 62, 141

Zárate S., 1999, Ethnobotany and domestication process of Leucaena in Mexico, Journal of Ethnobiology, 19, 1

10.2307/2656583

Thông tin
Thông tin xuất bản

American Journal of Botany

Tập 89 Số 7

1057-1073

Thông tin tác giả