Distribution of hypophysiotropic thyrotropin‐releasing hormone (TRH)‐synthesizing neurons in the hypothalamic paraventricular nucleus of the mouse

Journal of Comparative Neurology - Tập 518 Số 19 - Trang 3948-3961 - 2010
Andrea Kádár1, Edith Sánchez2, Gábor Wittmann1,2, Praful S. Singru2, Tamás Füzesi1, Alessandro Marsili3, P. Reed Larsen3, Zsolt Liposits1,4, Ronald M. Lechan5,2, Csaba Fekete1,2
1Department of Endocrine Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary 1083
2Tupper Research Institute and Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, Tufts Medical Center, Boston, Massachusetts 02111
3Thyroid Section, Division of Endocrinology, Diabetes, and Hypertension, Brigham and Women's Hospital, Boston, Massachusetts 02115
4Department of Neuroscience, Faculty of Information Technology, Pámány Péter Catholic University, Budapest, Hungary 1083
5Department of Neuroscience, Tufts University School of Medicine, Boston, Massachusetts 02111

Tóm tắt

AbstractHypophysiotropic thyrotropin‐releasing hormone (TRH) neurons, the central regulators of the hypothalamic‐pituitary‐thyroid axis, are located in the hypothalamic paraventricular nucleus (PVN) in a partly overlapping distribution with non‐hypophysiotropic TRH neurons. The distribution of hypophysiotropic TRH neurons in the rat PVN is well understood, but the localization of these neurons is unknown in mice. To determine the distribution and phenotype of hypophysiotropic TRH neurons in mice, double‐ and triple‐labeling experiments were performed on sections of intact mice, and mice treated intravenously and intraperitoneally with the retrograde tracer Fluoro‐Gold. TRH neurons were located in all parts of the PVN except the periventricular zone. Hypophysiotropic TRH neurons were observed only at the mid‐level of the PVN, primarily in the compact part. In this part of the PVN, TRH neurons were intermingled with oxytocin and vasopressin neurons, but based on their size, the TRH neurons were parvocellular and did not contain magnocellular neuropeptides. Co‐localization of TRH and cocaine‐ and amphetamine‐regulated transcript (CART) were observed only in areas where hypophysiotropic TRH neurons were located. In accordance with the morphological observations, hypothyroidism increased TRH mRNA content of neurons only at the mid‐level of the PVN. These data demonstrate that the distribution of hypophysiotropic TRH neurons in mice is vastly different from the pattern in rats, with a dominant occurrence of these neurosecretory cells in the compact part and adjacent regions at the mid‐level of the PVN. Furthermore, our data demonstrate that the organization of the PVN is markedly different in mice and rats. J. Comp. Neurol. 518:3948–3961, 2010. © 2010 Wiley‐Liss, Inc.

Từ khóa


Tài liệu tham khảo

10.1523/JNEUROSCI.05-01-00081.1985

10.1016/S0006-8993(99)01977-0

10.1016/j.yfrne.2007.04.002

Fekete C, 2000, Association of cocaine‐ and amphetamine‐regulated transcript‐immunoreactive elements with thyrotropin‐releasing hormone‐synthesizing neurons in the hypothalamic paraventricular nucleus and its role in the regulation of the hypothalamic‐pituitary‐thyroid axis during fasting, J Neurosci, 20, 9224, 10.1523/JNEUROSCI.20-24-09224.2000

10.1210/endo.142.6.8207

10.1159/000124943

10.1002/cne.903070402

10.1016/S0079-6123(06)53012-2

10.1210/endo-111-1-55

Lechan RM, 1992, Feedback regulation of thyrotropin‐releasing hormone gene expression by thyroid hormone in the hypothalamic paraventricular nucleus, CIBA Found Symp, 168, 144

Merchenthaler I, 1994, Mapping of thyrotropin‐releasing hormone (TRH) neuronal systems of rat forebrain projecting to the median eminence and the OVLT. Immunocytochemistry combined with retrograde labeling at the light and electron microscopic levels, Acta Biol Hung, 45, 361

10.1016/S0169-328X(00)00194-7

10.1074/jbc.M209532200

Peeters R, 2001, Regional physiological adaptation of the central nervous system deiodinases to iodine deficiency, Am J Physiol Endocrinol Metab, 281, E54, 10.1152/ajpendo.2001.281.1.E54

Reichlin S, 1989, TRH: historical aspects, Ann N Y Acad Sci, 553, 1, 10.1111/j.1749-6632.1989.tb46627.x

10.1126/science.3116669

10.1002/cne.22126

10.1002/cne.901940306

10.1016/S0014-5793(98)00543-2

10.1523/JNEUROSCI.19-10-j0006.1999

Weindl A, 1973, Frontiers of neuroendocrinology, 3

10.1002/cne.22180

Thông tin
Thông tin xuất bản

Journal of Comparative Neurology

Tập 518 Số 19

3948-3961

Thông tin tác giả