Dissociation of nuclear import cargo complexes by the protein Ran: a fluorescence correlation spectroscopy study

Comptes Rendus Biologies - Tập 328 - Trang 1073-1082 - 2005
Cécile Fradin1, David Zbaida2, Michael Elbaum2
1Department of Physics and Astronomy and Department of Biochemistry and Biomedical Sciences, 1280 Main St. W, Hamilton, ON, L8S4M1, Canada
2Department of Materials and Interfaces, Weizmann Institute of Sciences, Rehovot 76100, Israel

Tài liệu tham khảo

Pemberton, 1998, Transport routes through the nuclear pore complex, Curr. Opin. Cell Biol., 10, 392, 10.1016/S0955-0674(98)80016-1 Gorlich, 1999, Transport between the cell nucleus and the cytoplasm, Annu. Rev. Cell Dev. Biol., 15, 607, 10.1146/annurev.cellbio.15.1.607 Adam, 1999, Transport pathways of macromolecules between the nucleus and the cytoplasm, Curr. Opin. Cell Biol., 11, 402, 10.1016/S0955-0674(99)80056-8 Bayliss, 2000, The molecular mechanism of transport of macromolecules through nuclear pore complexes, Traffic, 1, 448, 10.1034/j.1600-0854.2000.010602.x Kunzler, 2001, Targeting of Ran: variation on a common theme?, J. Cell Sci., 114, 3233, 10.1242/jcs.114.18.3233 Kalab, 2002, Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts, Science, 295, 2452, 10.1126/science.1068798 Harel, 2004, Importin beta: conducting a much larger cellular symphony, Mol. Cell, 16, 319, 10.1016/S1097-2765(04)00647-1 Nachury, 2001, Importin β is a mitotic target of the small GTPase Ran in spindle assembly, Cell, 104, 95, 10.1016/S0092-8674(01)00194-5 Gruss, 2001, Ran induces spindle assembly by reversing the inhibitory effect of importin α on TPX2 activity, Cell, 104, 89, 10.1016/S0092-8674(01)00193-3 Zhang, 2000, Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts, Science, 288, 1429, 10.1126/science.288.5470.1429 Zhang, 2002, Concentration of Ran on chromatin induces decondensation, nuclear envelope formation and nuclear pore complex assembly, Eur. J. Cell Biol., 81, 623, 10.1078/0171-9335-00288 Di Fiore, 2003, Mammalian RanBP1 regulates centrosome cohesion during mitosis, J. Cell Sci., 116, 3399, 10.1242/jcs.00624 Casolari, 2004, Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization, Cell, 117, 427, 10.1016/S0092-8674(04)00448-9 Conti, 2001, Nucleocytoplasmic transport enters the atomic age, Curr. Opin. Cell Biol., 13, 310, 10.1016/S0955-0674(00)00213-1 Catimel, 2001, Biophysical characterization of interactions involving importin-alpha during nuclear import, J. Biol. Chem., 276, 34189, 10.1074/jbc.M103531200 Fanara, 2000, Quantitative analysis of nuclear localization signal (NLS)-importin alpha interaction through fluorescence depolarization. Evidence for auto-inhibitory regulation of NLS binding, J. Biol. Chem., 275, 21218, 10.1074/jbc.M002217200 Gorlich, 1995, Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope, Curr. Biol., 5, 383, 10.1016/S0960-9822(95)00079-0 Imamoto, 1995, A karyophilic protein forms a stable complex with cytoplasmic components prior to nuclear pore binding, J. Biol. Chem., 270, 8559, 10.1074/jbc.270.15.8559 Webb, 2001, Fluorescence correlation spectroscopy: inception, biophysical experimentations, and prospectus, Appl. Opt., 40, 3969, 10.1364/AO.40.003969 Medina, 2002, Fluorescence correlation spectroscopy for the detection and study of single molecules in biology, Bioessays, 24, 758, 10.1002/bies.10118 Rigler, 1993, Fluorescence correlation spectroscopy with high count rate and low background: analysis of translational diffusion, Eur. Biophys. J., 22, 169, 10.1007/BF00185777 Widengren, 1998, Fluorescence correlation spectroscopy as a tool to investigate chemical reactions in solutions and on cell surfaces, Cell Mol. Biol. (Noisy-le-Grand), 44, 857 Klebe, 1995, Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1, Biochemistry, 34, 639, 10.1021/bi00002a031 Nevo, 2003, A molecular switch between alternative conformational states in the complex of Ran and importin beta1, Nat. Struct. Biol., 10, 553, 10.1038/nsb940 Fradin, 2003, Fluorescence correlation spectroscopy close to a fluctuating membrane, Biophys. J., 84, 2005, 10.1016/S0006-3495(03)75009-7 Widengren, 1995, Fluorescence correlation spectroscopy of triplet states in solution: a theoretical and experimental study, J. Phys. Chem., 99, 13368, 10.1021/j100036a009 Magde, 1974, Fluorescence correlation spectroscopy. II. An experimental realization, Biopolymers, 13, 29, 10.1002/bip.1974.360130103 Brock, 2001, Fluorescence correlation microscopy: Fluorescence correlation spectroscopy in cell biology, 132 Hodel, 2001, Dissection of a nuclear localization signal, J. Biol. Chem., 276, 1317, 10.1074/jbc.M008522200 Riddick, 2005, A systems analysis of importin-α–β mediated nuclear protein import, J. Cell Biol., 168, 1027, 10.1083/jcb.200409024