Dissimilation of 1,2-propanediol by rumen micro-organisms

British Journal of Nutrition - Tập 29 Số 2 - Trang 317-330 - 1973
J. W. Czerkawski1, Grace Breckenridge1
1Hannah Research Institute

Tóm tắt

1. The main products of fermentation of 1,2-propanediol weren-propanol and propionic acid, but variable amounts of acetic acid and carbon dioxide were also formed. The concentrations of an intermediate propionaldehyde increased and then decreased.2. A tentative scheme is suggested, showing that 1,2-propanediol is first dehydrated to propionaldehyde, which is then reduced ton-propanol. The scheme also explains the formation of propionic and acetic acids and shows how the metabolism of 1,2-propanediol is related to that of rhamnose.3. Experiments with samples of rumen contents from animals on various diets showed that 1,2-propanediol was metabolized most rapidly when the animals were given molassed sugar-beet pulp. The rates of dissimilation of the diol increased with the concentration of rumen contents and with the concentration of substrate.4. The dissimilation of 1,2-propanediol by rumen micro-organisms resulted in an increased uptake of hydrogen. The metabolic hydrogen, arising from the inhibition of methane production by chloroform, appeared to be better utilized than the gaseous hydrogen.5. Oxygen gas did not affect the utilization of 1,2-propanediol, but the diol increased the uptake of oxygen by the rumen contents. The hydrogen and carbon balances were better when 1,2-propanediol was incubated anaerobically than in the presence of oxygen.

Từ khóa


Tài liệu tham khảo

10.1079/BJN19720077

Czerkawski, 1971, Lab. Pract., 20, 403

Noble R. C. & Czerkawski J. W. (1972). Analyst (in the Press).

10.1021/ac60128a032

Rudney, 1950, Arch Biochem., 29, 231

10.1042/bj0430099

10.1021/bi00794a025

Emery, 1967, J. Nutrition, 92, 348, 10.1093/jn/92.3.348

Kluyver, 1937, Enzymologia, 4, 7

10.1021/ac60154a022

10.3168/jds.S0022-0302(64)88848-2

Ladd, 1959, Biochem. J., 71, 365

10.1079/BJN19660086

Conway, 1962, Microdiffusion Analysis and Volumetric Error, 234

10.3168/jds.S0022-0302(60)90192-2

Shull, 1960, J. biol. Chem., 235, 551, 10.1016/S0021-9258(19)67902-0

Rudney, 1954, J. biol. Chem., 210, 361, 10.1016/S0021-9258(18)65461-4

Vogel, 1951, Practical Organic Chemistry

Czerkawski, 1970, Lab. Pract., 19, 717

Brownstein, 1961, J. biol. Chem., 236, 1199, 10.1016/S0021-9258(18)64267-X

10.1079/BJN19720125

10.1021/jf60155a002

10.1079/BJN19690009

Ting, 1964, Biochim. biophys. Acta, 89, 217

Clapperton, 1972, Proc. Nutr. Soc., 31, 55

Huff, 1959, J. biol. Chem., 234, 1060, 10.1016/S0021-9258(18)98129-9

Abeles, 1961, J. biol. Chem., 236, 2347, 10.1016/S0021-9258(18)64083-9

Czerkawski, 1968, Lab. Pract., 17, 994

Thông tin
Thông tin xuất bản

British Journal of Nutrition

Tập 29 Số 2

317-330

Thông tin tác giả