Directed Evolution of Barnase Stability Using Proteolytic Selection

Kwon, 1996, Relationship between thermal stability, degradation rate and expression yield of barnase variants in the periplasm of Escherichia coli, Protein Eng., 9, 1197, 10.1093/protein/9.12.1197

Dahiyat, 1999, In silico design for protein stabilization, Curr. Opin. Biotechnol., 10, 387, 10.1016/S0958-1669(99)80070-6

Gu, 1995, A phage display system for studying the sequence determinants of protein folding, Protein Sci., 4, 1108, 10.1002/pro.5560040609

O'Neil, 1995, Phage display: protein engineering by directed evolution, Curr. Opin. Struct. Biol., 5, 443, 10.1016/0959-440X(95)80027-1

Ruan, 1998, Stabilizing the subtilisin BPN′ pro-domain by phage display selection: how restrictive is the amino acid code for maximum protein stability?, Protein Sci., 7, 2345, 10.1002/pro.5560071111

Jung, 1999, Selection for improved protein stability by phage display, J. Mol. Biol., 294, 163, 10.1006/jmbi.1999.3196

Chakravarty, 2000, Protein stabilization through phage display, FEBS Letters, 476, 296, 10.1016/S0014-5793(00)01725-7

Linse, 2000, An EF-hand phage display study of calmodulin subdomain pairing, J. Mol. Biol., 296, 473, 10.1006/jmbi.1999.3452

Berggard, 2001, Fragment complementation studies of protein stabilization by hydrophobic core residues, Biochemistry, 40, 1257, 10.1021/bi0014812

Imoto, 1986, Unfolding rates of globular proteins determined by kinetics of proteolysis, J. Mol. Biol., 190, 647, 10.1016/0022-2836(86)90250-0

Kristensen, 1998, Proteolytic selection for protein folding using filamentous bacteriophages, Fold. Des., 3, 321, 10.1016/S1359-0278(98)00044-3

Sieber, 1998, Selecting proteins with improved stability by a phage-based method, Nature Biotechnol., 16, 955, 10.1038/nbt1098-955

Finucane, 1999, Core-directed protein design. I. An experimental method for selecting stable proteins from combinatorial libraries, Biochemistry, 38, 11604, 10.1021/bi990765n

Finucane, 1999, Core-directed protein design. II. Rescue of a multiply mutated and destabilized variant of ubiquitin, Biochemistry, 38, 11613, 10.1021/bi990766f

Hartley, 1989, Barnase and barstar: two small proteins to fold and fit together, Trends Biochem. Sci., 14, 450, 10.1016/0968-0004(89)90104-7

Schulga, 1992, Cloning of the gene encoding RNase binase from Bacillus intermedius 7P, Nucl. Acids Res., 20, 2375, 10.1093/nar/20.9.2375

Serrano, 1993, Step-wise mutation of barnase to binase. A procedure for engineering increased stability of proteins and an experimental analysis of the evolution of protein stability, J. Mol. Biol., 233, 305, 10.1006/jmbi.1993.1508

Meiering, 1992, Effect of active site residues in barnase on activity and stability, J. Mol. Biol., 225, 585, 10.1016/0022-2836(92)90387-Y

Nord, 1995, A combinatorial library of an alpha-helical bacterial receptor domain, Protein Eng., 8, 601, 10.1093/protein/8.6.601

Fersht, 1993, The sixth Datta Lecture. Protein folding and stability: the pathway of folding of barnase, FEBS Letters, 325, 5, 10.1016/0014-5793(93)81405-O

Bond, 1997, Characterization of residual structure in the thermally denatured state of barnase by simulation and experiment: description of the folding pathway, Proc. Natl Acad. Sci. USA, 94, 13409, 10.1073/pnas.94.25.13409

Matsuura, 1999, Evolutionary molecular engineering by random elongation mutagenesis, Nature Biotechnol., 17, 58, 10.1038/5232

Lee, 2001, Optimization of binding electrostatics: charge complementarity in the barnase–barstar protein complex, Protein Sci., 10, 362, 10.1110/ps.40001

Parsell, 1989, The structural stability of a protein is an important determinant of its proteolytic susceptibility in Escherichia coli, J. Biol. Chem., 264, 7590, 10.1016/S0021-9258(18)83275-6

Hamill, 1998, The effect of boundary selection on the stability and folding of the third fibronectin type III domain from human tenascin, Biochemistry, 37, 8071, 10.1021/bi9801659

Martin, 2001, In vitro selection of highly stabilized protein variants with optimized surface, J. Mol. Biol., 309, 717, 10.1006/jmbi.2001.4698

Perl, 1998, Conservation of rapid two-state folding in mesophilic, thermophilic and hyperthermophilic cold shock proteins, Nature Struct. Biol., 5, 229, 10.1038/nsb0398-229

Fersht, 2000, A kinetically significant intermediate in the folding of barnase, Proc. Natl Acad. Sci. USA, 97, 14121, 10.1073/pnas.260502597

Dalby, 1998, Folding intermediates of wild-type and mutants of barnase. II. Correlation of changes in equilibrium amide exchange kinetics with the population of the folding intermediate, J. Mol. Biol., 276, 647, 10.1006/jmbi.1997.1547

Perrett, 1995, Relationship between equilibrium amide proton exchange behavior and the folding pathway of barnase, Biochemistry, 34, 9288, 10.1021/bi00029a003

Sancho, 1992, An N-terminal fragment of barnase has residual helical structure similar to that in a refolding intermediate, J. Mol. Biol., 224, 749, 10.1016/0022-2836(92)90559-3

Wong, 2000, Towards a complete description of the structural and dynamic properties of the denatured state of barnase and the role of residual structure in folding, J. Mol. Biol., 296, 1257, 10.1006/jmbi.2000.3523

Horovitz, 1992, Alpha-helix stability in proteins. II. Factors that influence stability at an internal position, J. Mol. Biol., 227, 560, 10.1016/0022-2836(92)90907-2