Direct tissue analysis using matrix‐assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation

Journal of Mass Spectrometry - Tập 38 Số 7 - Trang 699-708 - 2003
Sarah Schwartz1, Michelle L. Reyzer1, Richard M. Caprioli1
1Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37232, USA

Tóm tắt

AbstractPractical guidelines for the preparation of tissue sections for direct analysis by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data analysis approaches with regards to direct tissue analysis are also discussed. Copyright © 2003 John Wiley & Sons, Ltd.

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