Direct detection of Blastocystis sp. in human faecal samples and subtype assignment using single strand conformational polymorphism and sequencing

Molecular and Cellular Probes - Tập 22 Số 1 - Trang 24-29 - 2008
Panagiotis G. Menounos1, Gregory Spanakos2, Νικόλαος Τέγος2, Constantine M Vassalos3, Chrissanthy Papadopoulou3, Nicholas Vakalis2
1Laboratory of Research, Nursing Military Academy, Athens, Greece
2Department of Parasitology, Entomology and Tropical Diseases, National School of Public Health, 196 Alexandras Ave, 11521 Athens, Greece
3Microbiology Department, Medical School, University of Ioannina, 45110‐Ioannina, Greece

Tóm tắt

Từ khóa


Tài liệu tham khảo

Brumpt, 1912, Blastocystis hominis n. sp. et formes voisines, Bull Soc Pathol Exot, 5, 725

Silberman, 1996, Human parasite finds taxonomic home, Nature, London, 380, 398, 10.1038/380398a0

Windsor, 2002, Incidence of Blastocystis hominis in faecal samples submitted for routine microbiological analysis, Br J Biomed Sci, 59, 154, 10.1080/09674845.2002.11783653

Stenzel, 1996, Blastocystis hominis revised, Clin Microbiol Rev, 9, 563, 10.1128/CMR.9.4.563

Belova, 1991, Blastocystis anatis sp. nov. (Rhizopoda, Lobosea) from Anas platyrhynchos, Zool Zh, 70, 5

Belova, 1990, Blastocystis galli sp. n. (Protista: Phizopoda) from the intestine of domestic hens, Parasitologiia, 24, 164

Pakandl, 1991, Occurence of Blastocystis sp. in pigs, Folia Parasitol, 38, 297

Quilez, 1995, Occurence of Blastocystis sp. in cattle in Aragon, northeastern Spain, Parasitol Res, 81, 703, 10.1007/BF00931851

Kaneda, 2001, Ribodemes of Blastocystis hominis isolated in Japan, Am J Trop Med Hyg, 65, 393, 10.4269/ajtmh.2001.65.393

Ho, 2001, Use of the elongation factor-1α gene in a polymerase chain reaction-based restriction-fragment-length polymorphism analysis of genetic heterogeneity among Blastocystis species, Mol Biochem Parasitol, 112, 287, 10.1016/S0166-6851(00)00358-3

Clark, 1997, Extensive genetic diversity in Blastocystis hominis, Mol Biochem Parasitol, 87, 79, 10.1016/S0166-6851(97)00046-7

Yoshikawa, 1996, DNA polymorphism revealed by arbitrary primers polymerase chain reaction among Blastocystis trains isolated from humans, a chicken and a reptile, J Eukaryot Microbiol, 43, 127, 10.1111/j.1550-7408.1996.tb04492.x

Yoshikawa, 1998, Genomic polymorphism among Blastocystis hominis strains and development of sybtype-specific diagnostic primers, Mol Cell Probes, 12, 153, 10.1006/mcpr.1998.0161

Yoshikawa, 2000, Genomic analysis of Blastocystis hominis strains isolated from two long-term health care facilities, J Clin Microbiol, 38, 1324, 10.1128/JCM.38.4.1324-1330.2000

Albrecht, 1995, Blastocystis hominis in human immunodeficiency virus-related diarrhea, Scand J Gastroenterol, 30, 909, 10.3109/00365529509101600

Carrascosa, 1996, Hemorrhagic proctosigmoiditis and Blastocystis hominis infection, Ann Intern Med, 124, 278, 10.7326/0003-4819-124-2-199601150-00031

Levy, 1996, Severe Blastocystis hominis in an elderly man, J Infect, 33, 57, 10.1016/S0163-4453(96)92833-9

Sun, 1989, Questionable clinical significance of Blastocystis hominis infection, Am J Gastroenterol, 84, 1543

Zaki, 1991, Clinical report of Blastocystis hominis infection in children, J Trop Med Hyg, 94, 118

Yakoob, 2004, Irritable bowel syndrome: in search of an etiology: role of Blastocystis hominis, Am J Trop Med Hyg, 70, 383, 10.4269/ajtmh.2004.70.383

Stark, 2007, Irritable bowel syndrome: a review on the role of intestinal protozoa and the importance of their detection and diagnosis, Int J Parasitol, 37, 11, 10.1016/j.ijpara.2006.09.009

Termmathurapoj, 2004, The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens, Parasitol Res, 93, 445, 10.1007/s00436-004-1157-x

Scicluna, 2006, DNA barcoding of Blastocystis, Protist, 57, 77, 10.1016/j.protis.2005.12.001

Abe, 2003, Molecular characterization of Blastocystis isolates from birds by PCR with diagnostic primers and restriction fragment length polymorphism analysis of the small subunit ribosomal RNA gene, Parasitol Res, 89, 393, 10.1007/s00436-002-0782-5

Abe, 2003, Molecular characterization of Blastocystis isolates from primates, Vet Parasitol, 113, 312, 10.1016/S0304-4017(03)00081-5

Arisue, 2003, Sequence heterogeneity of the small subunit ribosomal RNA genes among Blastocystis isolates, Parasitology, 126, 1, 10.1017/S0031182002002640

Clark, 2000, Cryptic genetic variation in parasitic protozoa, J Med Microbiol, 49, 489, 10.1099/0022-1317-49-6-489

Parkar, 2007, Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential, Parasitology, 134, 359, 10.1017/S0031182006001582

Stensvold, 2007, Terminology for Blastocystis subtypes—a consensus, Trends Parasitol, 23, 93, 10.1016/j.pt.2007.01.004

Yan, 2006, Genetic variability of Blastocystis hominis isolates in China, Parasitol Res, 99, 597, 10.1007/s00436-006-0186-z

Thompson, 1997, The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools, Nucleic Acids Res, 25, 4876, 10.1093/nar/25.24.4876

Rozen, 2000, Primer3 on the WWW for general users and for biologist programmers, 365

Orita, 1989, Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms, Proc Natl Acad Sci USA, 86, 2766, 10.1073/pnas.86.8.2766

Hayashi, 1991, PCR-SSCP: a simple sensitive method for detection of mutations in the genomic DNA, PCR Methods Appl, 1, 34, 10.1101/gr.1.1.34

Morgenstern, 1999, DIALIGN 2: improvement of the segment-to-segment approach to multiple sequence alignment, Bioinformatics, 15, 211, 10.1093/bioinformatics/15.3.211

Felsenstein, 1989, PHYLIP-phylogeny inference package (version 3.2), Cladistics, 5, 164

Altschul, 1997, Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res, 25, 3389, 10.1093/nar/25.17.3389

Leelayoova, 2002, In vitro cultivation: a sensitive method for detecting Blastocystis hominis, Ann Trop Med Parasitol, 96, 803, 10.1179/000349802125002275

Stensvold, 2006, Detection of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction, J Parasitol, 92, 1081, 10.1645/GE-840R.1

Yoshikawa, 2003, Molecular comparative studies among Blastocystis isolates obtained from humans and animals, J Parasitol, 89, 585, 10.1645/0022-3395(2003)089[0585:MCSABI]2.0.CO;2

Yoshikawa, 2004, PCR-based identification of zoonotic isolates of Blastocystis from mammals and birds, Microbiology, 150, 1147, 10.1099/mic.0.26899-0

Yoshikawa, 2004, Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries, Parasitol Res, 92, 22, 10.1007/s00436-003-0995-2

Noel, 2005, Molecular Phylogenies of Blastocystis isolates from different hosts: Implications for genetic diversity, identification of species and zoonosis, J Clin Microbiol, 43, 348, 10.1128/JCM.43.1.348-355.2005