Dimerisation strategies for shark IgNAR single domain antibody fragments

Albrecht, 2004, Production of soluble ScFvs with C-terminal-free thiol for site-specific conjugation or stable dimeric ScFvs on demand, Bioconjug. Chem., 15, 16, 10.1021/bc030018+ Ausubel, 1989 Bothmann, 2000, The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. I. Increased functional expression of antibody fragments with and without cis-prolines, J. Biol. Chem., 275, 17100, 10.1074/jbc.M910233199 Coia, 1997, Use of mutator cells as a means for increasing production levels of a recombinant antibody directed against Hepatitis B, Gene, 201, 203, 10.1016/S0378-1119(97)00452-6 Colcher, 1990, In vivo tumor targeting of a recombinant single-chain antigen-binding protein, J. Natl. Cancer Inst., 14, 1191, 10.1093/jnci/82.14.1191 Conrath, 2003, Emergence and evolution of functional heavy-chain antibodies in Camelidae, Dev. Comp. Immunol., 27, 87, 10.1016/S0145-305X(02)00071-X Deyev, 2003, Design of multivalent complexes using the barnase⁎barstar module, Nat. Biotechnol., 21, 1486, 10.1038/nbt916 Di Paolo, 2003, A recombinant immunotoxin derived from a humanized epithelial cell adhesion molecule-specific single-chain antibody fragment has potent and selective antitumor activity, Clin. Cancer Res., 9, 2837 Dolezal, 2000, ScFv multimers of the anti-neuraminidase antibody NC10: shortening of the linker in single-chain Fv fragment assembled in V(L) to V(H) orientation drives the formation of dimers, trimers, tetramers and higher molecular mass multimers, Protein Eng., 13, 565, 10.1093/protein/13.8.565 Dooley, 2003, Selection and characterization of naturally occurring single-domain (IgNAR) antibody fragments from immunized sharks by phage display, Mol. Immunol., 40, 25, 10.1016/S0161-5890(03)00084-1 Dorrington, 1976, Properties of the Fc receptor on macrophages, Immunol. Commun., 5, 263, 10.3109/08820137609044280 Els Conrath, 2001, Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs, J. Biol. Chem., 276, 7346, 10.1074/jbc.M007734200 Fraile, 2004, Secretion of proteins with dimerization capacity by the haemolysin type I transport system of Escherichia coli, Mol. Microbiol., 53, 1109, 10.1111/j.1365-2958.2004.04205.x Greenberg, 1995, A new antigen receptor gene family that undergoes rearrangement and extensive somatic diversification in sharks, Nature, 374, 168, 10.1038/374168a0 Harwood, 1987, Significance of circulatory clearance of tumour-localising IgG and F(ab')2 for potential therapy studied in a CEA-producing xenograft model, Tumour Biol., 8, 19, 10.1159/000217487 Hill, 1998, Solution structure of alpha-2D, a native-like de novo designed protein, J. Am. Chem. Soc., 120, 1138, 10.1021/ja9733649 Holliger, 2005, Engineered antibody fragments and the rise of single domains, Nat. Biotechnol., 23, 1126, 10.1038/nbt1142 Hoogenboom, 2005, Selecting and screening recombinant antibody libraries, Nat. Biotechnol., 23, 1105, 10.1038/nbt1126 Humphrey, W., Dalke, A., Schulten, K., 1996. VMD: visual molecular dynamics. J Mol Graph 14, 33-8, 27-8. Huston, 1988, Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli, Proc. Natl. Acad. Sci. U. S. A., 85, 5879, 10.1073/pnas.85.16.5879 Kiselyov, 2005, Structural biology of NCAM homophilic binding and activation of FGFR, J. Neurochem., 94, 1169, 10.1111/j.1471-4159.2005.03284.x Kortt, 2001, Dimeric and trimeric antibodies: high avidity scFvs for cancer targeting, Biomol. Eng., 18, 95, 10.1016/S1389-0344(01)00090-9 Kortt, 1999, Analysis of the binding of the Fab fragment of monoclonal antibody NC10 to influenza virus N9 neuraminidase from tern and whale using the BIAcore biosensor: effect of immobilization level and flow rate on kinetic analysis, Anal. Biochem., 273, 133, 10.1006/abio.1999.4183 Leader, 1991, Functional interactions of a glycosylated monoclonal anti-D with Fc gamma RI+and Fc gamma RIII+cells, Immunology, 72, 481 Lu, 2003, Di-diabody: a novel tetravalent bispecific antibody molecule by design, J. Immunol. Methods, 279, 219, 10.1016/S0022-1759(03)00251-5 Minsky, 1986, Secretion of beta-lactamase into the periplasm of Escherichia coli: evidence for a distinct release step associated with a conformational change, Proc. Natl. Acad. Sci. U. S. A., 83, 4180, 10.1073/pnas.83.12.4180 Muller, 1998, A dimeric bispecific miniantibody combines two specificities with avidity, FEBS Lett., 432, 45, 10.1016/S0014-5793(98)00829-1 Muyldermans, 2001, Single domain camel antibodies: current status, J. Biotechnol., 74, 277 Neri, 1995, High-affinity antigen binding by chelating recombinant antibodies (CRAbs), J. Mol. Biol., 246, 367, 10.1006/jmbi.1994.0091 Nuttall, 2001, Isolation of the new antigen receptor from wobbegong sharks, and use as a scaffold for the display of protein loop libraries, Mol. Immunol., 38, 313, 10.1016/S0161-5890(01)00057-8 Nuttall, 2002, A naturally occurring NAR variable domain binds the Kgp protease from Porphyromonas gingivalis, FEBS Lett., 516, 80, 10.1016/S0014-5793(02)02506-1 Nuttall, 2003, Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70, Eur. J. Biochem., 270, 3543, 10.1046/j.1432-1033.2003.03737.x Nuttall, 2004, Selection and affinity maturation of IgNAR variable domains targeting Plasmodium falciparum AMA1, Proteins, 55, 187, 10.1002/prot.20005 Pleschberger, 2004, An S-layer heavy chain camel antibody fusion protein for generation of a nanopatterned sensing layer to detect the prostate-specific antigen by surface plasmon resonance technology, Bioconjug. Chem., 15, 664, 10.1021/bc049964w Plückthun, 1997, New protein engineering approaches to multivalent and bispecific antibody fragments, Immunotechnology, 3, 83, 10.1016/S1380-2933(97)00067-5 Ronnmark, 2002, Construction and characterization of affibody-Fc chimeras produced in Escherichia coli, J. Immunol. Methods, 261, 199, 10.1016/S0022-1759(01)00563-4 Roux, 1998, Structural analysis of the nurse shark (new) antigen receptor (NAR): molecular convergence of NAR and unusual mammalian immunoglobulins, Proc. Natl. Acad. Sci. U. S. A., 95, 11804, 10.1073/pnas.95.20.11804 Rumfelt, 2004, Unprecedented multiplicity of Ig transmembrane and secretory mRNA forms in the cartilaginous fish, J. Immunol., 173, 1129, 10.4049/jimmunol.173.2.1129 Saphire, 2001, Crystal structure of a neutralizing human IGG against HIV-1: a template for vaccine design, Science, 293, 1155, 10.1126/science.1061692 Schmiedl, 2000, Expression of a bispecific dsFv–dsFv' antibody fragment in Escherichia coli, Protein Eng., 13, 725, 10.1093/protein/13.10.725 Steensgaard, 1977, The properties of immune complex-forming systems. A new theoretical approach, Immunology, 32, 445 Streltsov, 2004, Structural evidence for evolution of shark Ig new antigen receptor variable domain antibodies from a cell-surface receptor, Proc. Natl. Acad. Sci. U. S. A., 101, 12444, 10.1073/pnas.0403509101 Willuda, 2001, Tumor targeting of mono-, di-, and tetravalent anti-p185(HER-2) miniantibodies multimerized by self-associating peptides, J. Biol. Chem., 276, 14385, 10.1074/jbc.M011669200 Wright, 1997, Effect of glycosylation on antibody function: implications for genetic engineering, Trends Biotechnol., 15, 26, 10.1016/S0167-7799(96)10062-7 Wu, 2005, Arming antibodies: prospects and challenges for immunoconjugates, Nat. Biotechnol., 23, 1137, 10.1038/nbt1141 Zhang, 2004, Pentamerization of single-domain antibodies from phage libraries: a novel strategy for the rapid generation of high-avidity antibody reagents, J. Mol. Biol., 335, 49, 10.1016/j.jmb.2003.09.034