Diffusion tensor imaging correlates with cytopathology in a rat model of neonatal hydrocephalus

Cerebrospinal Fluid Research - Tập 7 Số 1 - 2010
Weihong Yuan1, Kelley E Deren2, James P. McAllister2, Scott K. Holland1, Diana M. Lindquist3, Alessandro Cancelliere4, Matthew S. Mason2, A. Duke Shereen3, Dean A. Hertzler4, Mekibib Altaye5, Francesco T. Mangano4
1Department of Radiology, Pediatric Neuroimaging Research Consortium, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, MLC 5033, 3333 Burnet Ave., Cincinnati, OH, 45229, USA
2Department of Neurosurgery, Division of Pediatric Neurosurgery, Primary Children's Medical Center and the University of Utah, N. Medical Drive East, Salt Lake City, UT, 84132, USA
3Department of Radiology, Imaging Research Center, Cincinnati Children's Hospital Medical Center and University of Cincinnati College of Medicine, MLC 5033, 3333 Burnet Ave., Cincinnati, OH, 45229, USA
4Division of Pediatric Neurosurgery, University of Cincinnati, Cincinnati Children's Hospital Medical Center MLC 2016, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA
5Department of Biostatistics & Epidemiology, Cincinnati Children's Hospital Medical Center, MLC 5041, 3333 Burnet Ave., Cincinnati, OH, 45229, USA

Tóm tắt

Abstract Background

Diffusion tensor imaging (DTI) is a non-invasive MRI technique that has been used to quantify CNS abnormalities in various pathologic conditions. This study was designed to quantify the anisotropic diffusion properties in the brain of neonatal rats with hydrocephalus (HCP) and to investigate association between DTI measurements and cytopathology.

 Methods

DTI data were acquired between postnatal day 7 (P7) and P12 in 12 rats with HCP induced at P2 and in 15 age-matched controls. Animals were euthanized at P11 or P22/P23 and brains were processed with immunohistochemistry for glial fibrillary acidic protein (GFAP), ionized calcium-binding adaptor molecule (Iba-1), and luxol fast blue (LFB) to assess astrocytosis, microglial reactivity and degree of myelination, respectively.

 Results

Hydrocephalic rats were consistently found to have an abnormally low (at corrected p-level of <0.05) fractional anisotropy (FA) value and an abnormally high mean diffusivity (MD) value in the cerebral cortex (CX), the corpus callosum (CC), and the internal capsule (IC). Immunohistochemical analysis demonstrated trends of increasing astrocyte and microglial reactivity in HCP rats at P11 that reached statistical significance at P22/P23. A trend toward reduced myelination in the HCP rats was also found at P22/P23. Correlation analysis at P11 for the CC demonstrated statistically significant correlations (or trends) between the DTI measurement (the decreased FA and increased MD values) and the GFAP or Iba-1 rankings. The immunohistochemical rankings in the IC at P22/P23 were also significantly correlated or demonstrated a trend with both FA and MD values.

 Conclusions

This study demonstrates the feasibility of employing DTI on the brain in experimental hydrocephalus in neonatal rats and reveals impairments in multiple regions of interest in both grey and white matter. A strong correlation was found between the immunohistochemical results and the changes in anisotropic diffusion properties.

Từ khóa


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Cerebrospinal Fluid Research

Tập 7 Số 1

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