Differentiation of LA-N-5 neuroblastoma cells into cholinergic neurons: methods for differentiation, immunohistochemistry and reporter gene introduction

Brain Research Protocols - Tập 2 - Trang 183-190 - 1998
David P Hill1, Kent A Robertson1
1Department of Pediatrics, Indiana University School of Medicine, Herman B Wells Center for Pediatric Research, Riley Hospital for Children, Room 2600, 702 Barnhill Drive, Indianapolis, IN 46202, USA

Tài liệu tham khảo

F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl, Immunohistochemistry, in: K. Janssen, (Ed.), Current Protocols in Molecular Biology, Vol. 2, Wiley and Sons, Boston, MA, 1994, p. 14.6. Breitman, 1980, Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid, Proc. Natl. Acad. Sci. USA, 77, 2936, 10.1073/pnas.77.5.2936 Forrester, 1996, An induction gene trap screen in embryonic stem cells: identification of genes that respond to retinoic acid in vitro, Proc. Natl. Acad. Sci. USA, 93, 1677, 10.1073/pnas.93.4.1677 Gomez, 1992, Identification of a MAP kinase kinase kinase in phaeochromocytoma (PC12) cells, FEBS Lett., 314, 461, 10.1016/0014-5793(92)81527-S Han, 1995, Enhanced potency of 9-cis versus all-trans retinoic acid to induce the differentiation of human neuroblastoma cells, Differentiation, 59, 61, 10.1046/j.1432-0436.1995.5910061.x Hill, 1997, Characterization of the cholinergic neuronal differentiation of the human neuroblastoma cell line LA-N-5 after treatment with retinoic acid, Dev. Brain Res., 102, 53, 10.1016/S0165-3806(97)00076-X Hill, 1990, Brief cytochalasin-induced disruption of microfilaments during a critical interval in 1-cell C. elegans embryos alters the partitioning of developmental instructions to the 2-cell embryo, Development, 108, 159, 10.1242/dev.108.1.159 D.P. Hill, W. Wurst, Gene and enhancer trapping: mutagenic strategies for developmental studies, in: R. Pedersen (Ed.), Current Topics in Developmental Biology, Vol. 28, Academic Press, San Diego, CA, 1993, pp. 181–206. D.P. Hill, W. Wurst, The use of gene-trap vectors to identify and mutate developmentally-regulated genes, in: G. Coupland (Ed.), Insertional Mutagenesis: a Practical Approach, Oxford University Press, Oxford, 1997, in press. Keller, 1995, In vitro differentiation of embryonic stem cells, Curr. Opin. Cell Biol., 7, 862, 10.1016/0955-0674(95)80071-9 W.D. Linscott, Linscott's Directory of Immunological and Biological Reagents, 9th Edn., Linscott, Santa Rosa, CA, 1996. Robson, 1985, Ultrastructural features of a human neuroblastoma cell line treated with retinoic acid, Neuroscience, 14, 1149, 10.1016/0306-4522(85)90284-2 Shigekawa, 1989, Electroporation: a general approach to the introduction of macromolecules into prokaryotic and eukaryotic cells, Aust. J. Biotechnol., 3, 56 Sidell, 1984, Regulation of acetylcholinesterase activity by retinoic acid in a human neuroblastoma cell line, Exp. Cell Res., 155, 305, 10.1016/0014-4827(84)90795-X Toneguzzo, 1988, Electric field-mediated gene transfer: characterization of DNA transfer and patterns of integration in lymphoid cells, Nucleic Acids Res., 16, 5515, 10.1093/nar/16.12.5515 Watson, 1993, Differential cDNA screening strategies to identify novel stage-specific proteins in the developing mammalian brain, Dev. Neurosci., 15, 77, 10.1159/000111319 Wurst, 1995, A large-scale gene-trap screen for insertional mutations in developmentally regulated genes in mice, Genetics, 139, 889, 10.1093/genetics/139.2.889