Differential gene expression profiling between genotoxic and non-genotoxic hepatocarcinogens in young rat liver determined by quantitative real-time PCR and principal component analysis

Kazuya Suenaga1, Hironao Takasawa2, Takashi Watanabe1, Yumi Wako2, Takayoshi Suzuki3, Shuichi Hamada2, Chie Furihata1,3
1Functional Genomics Laboratory, School of Science and Engineering, Aoyama Gakuin University, Fuchinobe 5-10-1, Chuo-ku, Sagamihara, Kanagawa 252-5258, Japan
2Safety Assessment Department, Mitsubishi Chemical Medience Corp., 14-1 Sunayama, Kamisu-shi, Ibaraki 314-0255, Japan
3Division of Cellular & Gene Therapy Products, National Institute of Health Sciences, Kamiyoga 1-18-1 Setagaya-ku, Tokyo 158-8501, Japan

Tài liệu tham khảo

Bolt, 2008, Strategy of the scientific committee on occupational exposure limits (SCOEL) in the derivation of occupational exposure limits for carcinogens and mutagens, Arch. Toxicol., 82, 61, 10.1007/s00204-007-0260-z Waters, 2010, Characterizing and predicting carcinogenicity and mode of action using conventional and toxicogenomics methods, Mutat. Res., 705, 184, 10.1016/j.mrrev.2010.04.005 Mathijs, 2009, Discrimination for genotoxic and nongenotoxic carcinogens by gene expression profiling in primary mouse hepatocytes improves with exposure time, Toxicol. Sci., 112, 374, 10.1093/toxsci/kfp229 Wu, 2009, A review of statistical methods for preprocessing oligonucleotide microarrays, Stat. Methods Med. Res., 18, 533, 10.1177/0962280209351924 Yauk, 2007, Review of the literature examining the correlation among DNA microarray technologies, Environ. Mol. Mutagen., 48, 380, 10.1002/em.20290 Calcagno, 2010, Analysis of expression of drug resistance-linked ABC transporters in cancer cells by quantitative RT-PCR, Methods Mol. Biol., 637, 121, 10.1007/978-1-60761-700-6_6 Nardon, 2009, Higher random oligo concentration improves reverse transcription yield of cDNA from bioptic tissues and quantitative RT-PCR reliability, Exp. Mol. Pathol., 87, 146, 10.1016/j.yexmp.2009.07.005 Watanabe, 2007, Differential gene expression induced by two genotoxic N-nitroso carcinogens, phenobarbital and ethanol in mouse liver examined with oligonucleotide microarray and quantitative real-time PCR, Gene Environ., 29, 115, 10.3123/jemsge.29.115 Watanabe, 2009, Dose-dependent alterations in gene expression in mouse liver induced by diethylnitrosamine and ethylnitrosourea and determined by quantitative real-time PCR, Mutat. Res., 673, 9, 10.1016/j.mrgentox.2008.11.004 Watanabe, 2012, Discrimination of genotoxic and non-genotoxic hepatocarcinogens by statistical analysis based on gene expression profiles in mouse liver as determined by quantitative real-time PCR, Mutat. Res., 742, 164, 10.1016/j.mrgentox.2012.04.011 Suzuki, 2005, Mutat. Res., 583, 133, 10.1016/j.mrgentox.2005.03.012 Takasawa, 2010, Evaluation of a liver micronucleus assay in young rats (III): a study using nine hepatotoxicants by the Collaborative Study Group for the Micronucleus Test (CSGMT)/Japanese Environmental Mutagen Society (JEMS)-Mammalian Mutagenicity Study Group (MMS), Mutat. Res., 698, 30, 10.1016/j.mrgentox.2010.02.009 U.S. Environmental Protection Agency, N-Nitrosodiethylamine, CASRN 55-18-5 in Integrated Risk Information System, http://www.epa.gov/iris/subst/0042.htm. U.S. Environmental Protection Agency, Di(2-ethylhexyl)phthalate (DEHP), CASRN 117-81-7 (03/01/1997) in Integrated Risk Information System, http://www.epa.gov/iris/subst/0014.htm. U.S. Environmental Protection Agency, 2,4-/2,6-Dinitrotoluene mixture in Integrated Risk Information System, http://www.epa.gov/iris/subst/0397.htm. National Toxicology Program, Department of Health and Human Services, Phenacetin and Analgesic Mixtures Containing Phenacetin in Report on Carcinogens, Twelfth Edition, http://ntp.niehs.nih.gov/ntp/roc/twelfth/profiles/PhenacetinAndAnalgesicMixtures.pdf (2011). Calder, 1976, J.D. Neoplasia in the rat induced by N-hydroxyphenacetin, a metabolite of phenacetin, Pathology, 8, 1, 10.3109/00313027609094418 Suzuki, 2011, Genotoxicity studies of 2,6-dinitrotoluene (2,6-DNT), J. Toxicol. Sci., 36, 499, 10.2131/jts.36.499 Camus, 1982, Species-specific activation of phenacetin into bacterial mutagens by hamster liver enzymes and identification of N-hydroxyphenacetin O-glucuronide as a promutagen in the urine, Cancer Res., 42, 3201 Sawada, 1989, In vivo short-term assays of repair and replication of rat liver DNA, J. Cancer Res. Clin. Oncol., 115, 345, 10.1007/BF00400961 Asakura, 1994, Effects of dietary restriction on induction of unscheduled DNA synthesis (UDS) and replicative DNA synthesis (RDS) in rat liver, Mutat. Res., 322, 257, 10.1016/0165-1218(94)90101-5 Sawada, 1995, Comparison of autoradiography, liquid scintillation counting and immunoenzymatic staining of 5-bromo-2′-deoxyuridine for measurement of unscheduled DNA synthesis and replicative DNA synthesis in rat liver, Mutat. Res., 344, 109, 10.1016/0165-1218(95)00039-9 Tsuda, 2000, The comet assay in eight mouse organs: results with 24 azo compounds, Mutat. Res., 465, 11, 10.1016/S1383-5718(99)00199-0 Uno, 1992, In vivo-in vitro replicative DNA synthesis (RDS) test using perfused rat livers as an early prediction assay for non-genotoxic hepatocarcinogens: II. Assessment of judgement criteria, Toxicol. Lett., 63, 201, 10.1016/0378-4274(92)90012-9 Copple, 2004, Modes of cell death in rat liver after monocrotaline exposure, Toxicological Sciences, 77, 172, 10.1093/toxsci/kfh011 Ellinger-Ziegelbauer, 2005, Comparison of the expression profiles induced by genotoxic and non-genotoxic carcinogens in rat liver, Mutat. Res., 575, 61, 10.1016/j.mrfmmm.2005.02.004