Biểu hiện khác biệt của enzyme tổng hợp axit béo (FAS) và ErbB2 trong tế bào biểu mô miệng không ác tính và ác tính

Sabrina D. Silva1, Isabela W. Cunha2, Ana Lúcia C. A. Rangel1, Jacks Jorge1, Karina G. Zecchin3, Michelle Agostini1, Luiz P. Kowalski4, Ricardo D. Coletta1, Edgard Graner1
1Departament of Oral Diagnosis, School of Dentistry of Piracicaba, State University of Campinas (UNICAMP), Piracicaba, Brazil
2Departament of Anatomical Pathology, A.C. Camargo Cancer Hospital, São Paulo, Brazil
3Department of Clinical Pathology, School of Medical Sciences, State University of Campinas, UNICAMP, Campinas, Brazil
4Department of Head and Neck Surgery and Otorhinolaryngology, A.C. Camargo Cancer Hospital, São Paulo, Brazil

Tóm tắt

Mục tiêu của nghiên cứu này là điều tra sự biểu hiện của enzyme tổng hợp axit béo (FAS) và ErbB2 trong biểu mô miệng không ác tính và các khối u carcinomas biểu mô vảy ở miệng hoặc vùng đầu-cổ (OSCC/HNSCC). Các mẫu biểu mô miệng bình thường, tăng sừng, và loạn sản cũng như các khối u OSCC phân biệt tốt và phân biệt kém đều được đánh giá bằng phương pháp nhuộm miễn dịch hóa học đối với FAS, ErbB2 và Ki-67. Những protein này cũng được phân tích trong một lớp vi mô mô với 55 mẫu HNSCC. Các tế bào SCC-9 được sử dụng để nghiên cứu sự biểu hiện của FAS và ErbB2 trong quá trình phân biệt. Sự biểu hiện của FAS cao hơn ở tình trạng tăng sừng, loạn sản, và OSCC so với biểu mô bình thường. Các khối u OSCC/HNSCC phân biệt tốt có sự biểu hiện FAS nhiều hơn so với các khối u phân biệt kém. ErbB2 được phát hiện ở bề mặt của các keratinocytes không ác tính và các khối u OSCC/HNSCC phân biệt tốt, cũng như trong tế bào chất của các tế bào phân biệt kém. Chỉ số Ki-67 tăng dần từ biểu mô miệng bình thường đến OSCC, có tương quan nghịch với ErbB2 tại bề mặt tế bào và có tương quan dương với ErbB2 nội bào. Cuối cùng, các mẫu nuôi cấy tế bào SCC-9 đã được làm giàu với các tế bào tích cực với ErbB2 màng sau khi phân biệt qua việc loại bỏ điểm tựa. Kết luận, FAS được biểu hiện quá mức trong OSCC/HNSCC và biểu mô miệng tăng sừng, còn ErbB2 có mặt ở bề mặt của các keratinocytes đang phân biệt và trong tế bào chất của các tế bào khối u phân biệt kém. Chỉ số Ki-67 cao hơn trong các loạn sản biểu mô và OSCC so với biểu mô miệng bình thường về mặt hình thái.

Từ khóa

#biểu hiện phân biệt #enzyme tổng hợp axit béo #FAS #ErbB2 #tế bào biểu mô miệng #khối u carcinomas biểu mô vảy

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