Differential expression of cell surface glycoproteins on various organ‐derived microvascular endothelia and endothelial cell cultures

Journal of Cellular Physiology - Tập 136 Số 3 - Trang 398-410 - 1988
Paula Belloni1, Garth L. Nicolson1
1Department of Tumor Biology, The University of Texas M.D. Anderson Hospital and Tumor Institue, Houston, Texas, 77030

Tóm tắt

AbstractGlycoproteins expressed on the luminal surfaces of microvascular endothelium derived from various murine organs were analyzed and compared with those expressed by cultured vascular endothelial cells. Cell‐surface vascular proteins were radiolabeled in situ via intracardiac perfusion with lactoperoxidase/Na125l. Autoradiography confirmed that the radiolabel was restricted to the vessel lumen in most tissues. Controls contained 125l‐labeled serum proteins to identify adsorbed serum components. Glycoproteins were analyzed by western enzymelinked lectin analysis using detergent extracts of 125l‐labeled microvessels isolated from different organs. The western transfers were probed with a panel of lectin‐peroxidase conjugates to determine differences in protein glycosylation. The same transfers were also screened for exposed 125l‐labeled cell‐surface proteins by autoradiography. This dual analysis detected glycoprotein patterns unique for each organ. At least seven major proteins (Mr ∼ 180 K, 130 K, 95 K, 80 K, 75 K, 60 K, 12 K) were common to microvessels derived from each organ; however, certain glycoproteins appeared to be expressed differentially in particular organs. For example, a Mr ∼ 135 K WGA‐binding glycoprotein was detected in brain microvessels, whereas another WGA‐binding glycoprotein of Mr ∼ 40 K was detected only in kidney. In lung microvessels, a Mr ∼ 140 K WGA binding glycoprotein and a Mr ∼ 55 K RCA‐l‐binding galactoprotein were expressed preferentially, and liver microvessels displayed Mr ∼ 220 K protein and a Mr ∼ 135 K PNA‐binding galactoprotein. The cell‐surface‐iodinated protein profiles from in situ labeled microvessels were similar to profiles derived from cultured bovine aortic endothelial cells and several short‐term endothelial cell cultures isolated from different organs. The results from this study suggest that organ‐associated endothelia express glycoprotein fingerprints unique to each organ.

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Journal of Cellular Physiology

Tập 136 Số 3

398-410

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