Kích hoạt khác biệt của gia đình kinase protein liên quan đến sucrose Nonfermenting1 tại gạo bởi áp lực căng thẳng thẩm thấu cao và axit abscisic[W]
Tóm tắt
Cho đến nay, một số lượng lớn các chuỗi protein kinase thuộc gia đình protein kinase liên quan đến sucrose nonfermenting1 (SnRK2) đã được tìm thấy trong cơ sở dữ liệu. Tuy nhiên, chỉ có một số lượng giới hạn các thành viên trong gia đình đã được mô tả đặc điểm và gắn liền với tín hiệu axit abscisic (ABA) và căng thẳng thẩm thấu cao. Chúng tôi đã xác định được 10 protein kinase SnRK2 mã hóa bởi bộ gen của gạo (Oryza sativa). Mỗi thành viên trong số 10 thành viên này được biểu hiện trong tế bào chất của tế bào nuôi cấy và quy định của chúng đã được phân tích. Tại đây, chúng tôi chứng minh rằng tất cả các thành viên trong gia đình đều được kích hoạt bởi căng thẳng thẩm thấu cao và ba trong số đó cũng được kích hoạt bởi ABA. Đáng ngạc nhiên là không có thành viên nào chỉ được kích hoạt bởi ABA. Việc kích hoạt được tìm thấy là thông qua quá trình phosphoryl hóa. Bên cạnh sự phân biệt chức năng liên quan đến quy định ABA, sự phụ thuộc vào căng thẳng thẩm thấu mạnh cũng khác nhau giữa các thành viên. Chúng tôi cho thấy rằng phần đuôi C tương đối khác biệt là nguyên nhân chính cho sự phân biệt chức năng này, mặc dù phần kinase cũng đóng góp cho những khác biệt này. Kết quả này chỉ ra rằng gia đình protein kinase SnRK2 đã tiến hóa đặc biệt cho tín hiệu căng thẳng thẩm thấu cao và rằng các thành viên cá nhân đã có được những tính chất điều tiết đặc biệt, bao gồm cả phản ứng ABA bằng cách điều chỉnh phần đuôi C.
Từ khóa
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