Development of an Integrated Biochip System Consisting of a Magnetic Particle Washing Station and a Markerless Volumetric Biochip
Tóm tắt
As the use of markers in conventional volumetric biochips results in complex biochip structures and assay processes, an integrated biochip system consisting of a magnetic particle (MP) washing station and a markerless volumetric biochip was developed in this study. The biochip was composed of a reaction chamber and a channel connected to the upper side of the chamber. The assay process involved the injection of a sample containing a catalase-labeled analyte into the reaction chamber containing a substrate solution comprising H2O2, detergent, and methylene blue; and the measurement of the distance traveled by the generated foam. The MP washing station had two check valves installed at different ports of a T-connector in opposite directions. Contaminants were removed from the MPs by pressing and releasing the pipette plunger, which caused a one-way flow of washing buffer from the buffer chamber to the waste chamber through the pipette. The integrated system was applied for the detection of Salmonella typhimurium using magnetic capture particles (CPs) functionalized with anti-S. typhimurium antibodies and labeling particles (LPs) functionalized with catalase and antibodies. After the bacterial sample was mixed with CPs and LPs, the resulting CP–bacteria–LP complex was washed in the MP washing station and analyzed in the markerless volumetric biochip. This method enabled the detection of bacteria with a limit of detection of 1.6 CFU within 25 min. Considering the high sensitivity and simplicity of the biochip structure and the assay process, the integrated system demonstrates considerable potential for the point-of-care testing of various analytes.
Tài liệu tham khảo
Bhat, M.P., Thendral, V., Uthappa, U.T., Lee, K.H., Kigga, M., Altalhi, T., Kurkuri, M.D., Kant, K.: Recent advances in microfluidic platform for physical and immunological detection and capture of circulating tumor cells. Biosensors 12, 220 (2022). https://doi.org/10.3390/BIOS12040220
Mane, S., Hemadri, V., Tripathi, S.: Separation of white blood cells in a wavy type microfluidic device using blood diluted in a hypertonic saline solution. BioChip J. 16(3), 291–304 (2022). https://doi.org/10.1007/S13206-022-00074-Z
Kim, H.R., Bong, J.H., Jung, J., Sung, J.S., Kang, M.J., Park, J.G., Pyun, J.C.: An On-chip chemiluminescent immunoassay for bacterial detection using in situ-synthesized cadmium sulfide nanowires with passivation layers. BioChip J. 14, 268–278 (2020). https://doi.org/10.1007/S13206-020-4305-1/FIGURES/6
Cai, G., Wang, S., Zheng, L., Lin, J.: A fluidic device for immunomagnetic separation of foodborne bacteria using self-assembled magnetic nanoparticle chains. Micromachines 9, 624 (2018). https://doi.org/10.3390/MI9120624
Lee, W.S., Ahn, J., Jung, S., Lee, J., Kang, T., Jeong, J.: Biomimetic nanopillar-based biosensor for label-free detection of influenza A virus. BioChip J. 15, 260–267 (2021). https://doi.org/10.1007/S13206-021-00027-Y/FIGURES/4
Arega, N.G., Heard, W.N., Tran, N.A.N., Jung, S., Meng, J., Chung, M., Kim, M.S., Kim, D.: Zinc-finger-protein-based microfluidic electrophoretic mobility reversal assay for quantitative double-stranded DNA analysis. BioChip J. 15, 381–395 (2021). https://doi.org/10.1007/S13206-021-00038-9/TABLES/1
Gao, N., Chang, J., Zhu, Z., You, H.: Multistory stairs-based, fast and point-of-care testing for disease biomarker using one-step capillary microfluidic fluoroimmunoassay chip via continuous on-chip labelling. BioChip J. 15, 268–275 (2021). https://doi.org/10.1007/S13206-021-00025-0/FIGURES/5
Kang, S.M.: Recent advances in microfluidic-based microphysiological systems. BioChip J. 16, 13–26 (2022). https://doi.org/10.1007/S13206-021-00043-Y/FIGURES/4
Liu, D., Tian, T., Chen, X., Lei, Z., Song, Y., Shi, Y., Ji, T., Zhu, Z., Yang, L., Yang, C.: Gas-generating reactions for point-of-care testing. Analyst 143, 1294–1304 (2018). https://doi.org/10.1039/C8AN00011E
Tian, T., Li, J., Song, Y., Zhou, L., Zhu, Z., Yang, C.J.: Distance-based microfluidic quantitative detection methods for point-of-care testing. Lab Chip 16, 1139–1151 (2016). https://doi.org/10.1039/C5LC01562F
Song, Y., Zhang, Y., Bernard, P.E., Reuben, J.M., Ueno, N.T., Arlinghaus, R.B., Zu, Y., Qin, L.: Multiplexed volumetric bar-chart chip for point-of-care diagnostics. Nat. Commun. 3, 1283–1289 (2012). https://doi.org/10.1038/ncomms2292
Xie, Y., Wei, X., Yang, Q., Guan, Z., Liu, D., Liu, X., Zhou, L., Zhu, Z., Lin, Z., Yang, C.: A shake & read distance-based microfluidic chip as a portable quantitative readout device for highly sensitive point-of-care testing. Chem. Commun. 52, 13377–13380 (2016). https://doi.org/10.1039/C6CC07928H
Lee, S., Kwon, D., Yim, C., Jeon, S.: Facile detection of troponin i using dendritic platinum nanoparticles and capillary tube indicators. Anal. Chem. 87, 5004–5008 (2015). https://doi.org/10.1021/acs.analchem.5b00921
Wu, Z., Fu, Q., Yu, S., Sheng, L., Xu, M., Yao, C., Xiao, W., Li, X., Tang, Y.: Pt@AuNPs integrated quantitative capillary-based biosensors for point-of-care testing application. Biosens. Bioelectron. 85, 657–663 (2016). https://doi.org/10.1016/j.bios.2016.05.074
Bu, S., Wang, K., Ju, C., Wang, C., Li, Z., Hao, Z., Shen, M., Wan, J.: Point-of-care assay to detect foodborne pathogenic bacteria using a low-cost disposable medical infusion extension line as readout and MNO2 nanoflowers. Food Control 98, 399–404 (2019). https://doi.org/10.1016/j.foodcont.2018.11.053
Huang, T., Yang, J., Zhou, W., Liu, X., Pan, Y., Song, Y.: Rapid identification of urinary tract infections based on ultrasensitive bacteria detection using volumetric bar-chart chip. Sens. Actuators B 298, 126885 (2019). https://doi.org/10.1016/j.snb.2019.126885
Liu, D., Li, X., Zhou, J., Liu, S., Tian, T., Song, Y., Zhu, Z., Zhou, L., Ji, T., Yang, C.: A fully integrated distance readout ELISA-chip for point-of-care testing with sample-in-answer-out capability. Biosens. Bioelectron. 96, 332–338 (2017). https://doi.org/10.1016/j.bios.2017.04.044
Li, Y., Uddayasankar, U., He, B., Wang, P., Qin, L.: Fast, sensitive, and quantitative point-of-care platform for the assessment of drugs of abuse in urine, serum, and whole blood. Anal. Chem. 89, 8273–8281 (2017). https://doi.org/10.1021/acs.analchem.7b01288
Song, Y., Wang, Y., Qi, W., Li, Y., Xuan, J., Wang, P., Qin, L.: Integrative volumetric bar-chart chip for rapid and quantitative point-of-care detection of myocardial infarction biomarkers. Lab Chip 16, 2955–2962 (2016). https://doi.org/10.1039/C6LC00561F
Han, H., Choi, S.-J.: Development of an inkless, visual volumetric chip operated with a micropipette. BioChip J. 15, 1–8 (2021). https://doi.org/10.1007/s13206-021-00021-4
Kim, M.-H., Choi, S.-J.: Immunoassay of paralytic shellfish toxins by moving magnetic particles in a stationary liquid-phase lab-on-a-chip. Biosens. Bioelectron. 66, 136–140 (2015). https://doi.org/10.1016/j.bios.2014.11.012
Williams, J.: The decomposition of hydrogen peroxide by liver catalase. J. Gen. Physiol. 11, 309–337 (1928). https://doi.org/10.1085/jgp.11.4.309
Lardinois, O.M., Mestdagh, M.M., Rouxhet, P.G.: Reversible inhibition and irreversible inactivation of catalase in presence of hydrogen peroxide. Biochim. Biophys. Acta Protein Struct. Mol. Enzymol. 1295, 222–238 (1996). https://doi.org/10.1016/0167-4838(96)00043-X
Beers, R.F., Sizer, I.W.: A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J. Biol. Chem. 195, 133–140 (1952). https://doi.org/10.1016/s0021-9258(19)50881-x
Hadwan, M.H.: Simple spectrophotometric assay for measuring catalase activity in biological tissues. BMC Biochem. 19, 7 (2018). https://doi.org/10.1186/s12858-018-0097-5
Hadwan, M.H., Ali, S.K.: New spectrophotometric assay for assessments of catalase activity in biological samples. Anal. Biochem. 542, 29–33 (2018). https://doi.org/10.1016/j.ab.2017.11.013
Mueller, S., Riedel, H.D., Stremmel, W.: Determination of catalase activity at physiological hydrogen peroxide concentrations. Anal. Biochem. 245, 55–60 (1997). https://doi.org/10.1006/abio.1996.9939
Shivakumar, A., Nagaraja, P., Chamaraja, N.A., Krishna, H., Avinash, K.: Determination of catalase activity using chromogenic probe involving iso-nicotinicacidhydrazide and pyrocatechol. J. Biotechnol. 155, 406–411 (2011). https://doi.org/10.1016/j.jbiotec.2011.07.035
Farman, A.A., Hadwan, M.H.: Simple kinetic method for assessing catalase activity in biological samples. MethodsX 8, 101434 (2021). https://doi.org/10.1016/j.mex.2021.101434
Hadwan, M.H.: New method for assessment of serum catalase activity. Indian J. Sci. Technol. 9, 1–5 (2016)