Development of a salivary cortisol method for detecting changes in plasma “free” cortisol arising from acute stress in sheep

Australian Veterinary Journal - Tập 62 Số 12 - Trang 403-406 - 1985
L. R. Fell1, DA Shutt1, C. J. BENTLEY1
1Department of Agriculture, Hawkesbury Agricultural Research Unit, Richmond, New South Wales 2753

Tóm tắt

SUMMARY: A simple device for collecting saliva (mainly parotid) from sheep is described. The collection of saliva, and the assay of “free” cortisol in saliva appears to offer certain advantages over the collection of blood, and the assay of serum cortisol, for the assessment of stress in sheep. With a little experience, it is easier to collect saliva than take blood samples when sheep are passing through a race. The “free” cortisol can be measured directly in saliva, whereas in serum, it is first necessary to separate “free” from protein‐bound cortisol. Basal levels of “free” cortisol of less than 10 nmol/1 were recorded in saliva and blood plasma or serum in unstressed sheep which had previous experience of being handled in a race, Significant increases in salivary cortisol and “free” and total (“free” plus protein‐bound) cortisol in serum were found in sheep following adrenal stimulation with synacthen, or after 30 min of stressful transport. This indicates that the salivary cortisol technique is applicable to studies of stress in sheep, and should also be useful for other ruminants.

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Australian Veterinary Journal

Tập 62 Số 12

403-406

Thông tin tác giả