Development of a novel platform for recombinant protein production in Corynebacterium glutamicum on ethanol

Kesik-Brodacka, 2018, Progress in biopharmaceutical development, Biotechnol Appl Biochem, 65, 306, 10.1002/bab.1617 Walsh, 2018, Biopharmaceutical benchmarks 2018, Nat Biotechnol, 36, 1136, 10.1038/nbt.4305 Luis Corchero, 2013, Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics, Biotechnol Adv, 31, 140, 10.1016/j.biotechadv.2012.09.001 Berlec, 2013, Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells, J Ind Microbiol Biotechnol, 40, 257, 10.1007/s10295-013-1235-0 Rosano, 2019, New tools for recombinant protein production in Escherichia coli: a 5-year update, Protein Sci, 28, 1412, 10.1002/pro.3668 Freudl, 2017, Beyond amino acids: use of the Corynebacterium glutamicum cell factory for the secretion of heterologous proteins, J Biotechnol, 258, 101, 10.1016/j.jbiotec.2017.02.023 Anne, 2017, Protein secretion in gram-positive bacteria: from multiple pathways to biotechnology, vol. 404, 267 Liu, 2013, Recent advances in recombinant protein expression by Corynebacterium, Brevibacterium, and Streptomyces: from transcription and translation regulation to secretion pathway selection, Appl Microbiol Biotechnol, 97, 9597, 10.1007/s00253-013-5250-x Harwood, 2008, Bacillus protein secretion: an unfolding story, Trends Microbiol, 16, 73, 10.1016/j.tim.2007.12.001 Wendisch, 2016, Updates on industrial production of amino acids using Corynebacterium glutamicum, World J Microbiol Biotechnol, 32, 105, 10.1007/s11274-016-2060-1 Lee, 2018, Recombinant protein expression system in Corynebacterium glutamicum and its application, Front Microbiol, 9, 2523, 10.3389/fmicb.2018.02523 Xu, 2010, An improved shuttle vector constructed for metabolic engineering research in Corynebacterium glutamicum, Plasmid, 64, 85, 10.1016/j.plasmid.2010.05.004 Yim, 2013, Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum, Biotechnol Bioeng, 110, 2959, 10.1002/bit.24954 Li, 2020, Obtaining a series of native gradient promoter-5 '-UTR sequences in Corynebacterium glutamicum ATCC 13032, Microb Cell Factories, 19, 120, 10.1186/s12934-020-01376-3 Okibe, 2010, Isolation, evaluation and use of two strong, carbon source-inducible promoters from Corynebacterium glutamicum, Lett Appl Microbiol, 50, 173, 10.1111/j.1472-765X.2009.02776.x Ata, 2017, Transcriptional engineering of the glyceraldehyde-3-phosphate dehydrogenase promoter for improved heterologous protein production in Pichia pastoris, Biotechnol Bioeng, 114, 2319, 10.1002/bit.26363 Ergun, 2019, Engineering of alcohol dehydrogenase 2 hybrid-promoter architectures in Pichia pastoris to enhance recombinant protein expression on ethanol, Biotechnol Bioeng, 116, 2674, 10.1002/bit.27095 Hartner, 2008, Promoter library designed for fine-tuned gene expression in Pichia pastoris, Nucleic Acids Res, 36, e76, 10.1093/nar/gkn369 Arndt, 2008, Ethanol catabolism in Corynebacterium glutamicum, J Mol Microbiol Biotechnol, 15, 222 Patek, 2013, Corynebacterium glutamicum promoters: a practical approach, Microb Biotechnol, 6, 103, 10.1111/1751-7915.12019 Ruan, 2015, Improving the electro-transformation efficiency of Corynebacterium glutamicum by weakening its cell wall and increasing the cytoplasmic membrane fluidity, Biotechnol Lett, 37, 2445, 10.1007/s10529-015-1934-x Zhao, 2016, Construction of genetic parts from the Corynebacterium glutamicum genome with high expression activities, Biotechnol Lett, 38, 2119, 10.1007/s10529-016-2196-y Gibson, 2009, Enzymatic assembly of DNA molecules up to several hundred kilobases, Nat Methods, 6, 10.1038/nmeth.1318 Engler, 2008, A one pot, one step, precision cloning method with high throughput capability, PLoS One, 3, e3647, 10.1371/journal.pone.0003647 Jiang, 2008, SeqMap: mapping massive amount of oligonucleotides to the genome, Bioinformatics, 24, 2395, 10.1093/bioinformatics/btn429 Wang, 2018, Identification and validation of appropriate reference genes for qRT-PCR analysis in Corynebacterium glutamicum, FEMS Microbiol Lett, 365, 10.1093/femsle/fny030 Liu, 2016, Expression of recombinant protein using Corynebacterium Glutamicum: progress, challenges and applications, Crit Rev Biotechnol, 36, 652, 10.3109/07388551.2015.1004519 Reinscheid, 1994, Characterization of the isocitrate lyase gene from corynebacterium-glutamicum and biochemical-analysis of the enzyme, J Bacteriol, 176, 3474, 10.1128/jb.176.12.3474-3483.1994 Reinscheid, 1994, Malate synthase from Corynebacterium glutamicum: sequence analysis of the gene and biochemical characterization of the enzyme, Microbiology-Sgm, 140, 3099, 10.1099/13500872-140-11-3099 Maeda, 2012, 3 ' untranslated region-dependent degradation of the aceA mRNA, encoding the glyoxylate cycle enzyme isocitrate lyase, by RNase E/G in Corynebacterium glutamicum, Appl Environ Microbiol, 78, 8753, 10.1128/AEM.02304-12 Cetnar, 2021, Systematic quantification of sequence and structural determinants controlling mRNA stability in bacterial operons, ACS Synth Biol, 10, 318, 10.1021/acssynbio.0c00471 Sun, 2020, Enhanced production of recombinant proteins in Corynebacterium glutamicum by constructing a bicistronic gene expression system, Microb Cell Factories, 19, 113, 10.1186/s12934-020-01370-9 Liu, 2020, Identification, repair and characterization of a benzyl alcohol-inducible promoter for recombinant proteins overexpression in Corynebacterium glutamicum, Enzym Microb Technol, 141, 10.1016/j.enzmictec.2020.109651 Liu, 2017, Bicistronic expression strategy for high-level expression of recombinant proteins in Corynebacterium glutamicum, Eng Life Sci, 17, 1118, 10.1002/elsc.201700087 Mutalik, 2013, Precise and reliable gene expression via standard transcription and translation initiation elements, Nat Methods, 10, 354, 10.1038/nmeth.2404 Gerstmeir, 2003, Acetate metabolism and its regulation in Corynebacterium glutamicum, J Biotechnol, 104, 99, 10.1016/S0168-1656(03)00167-6 Toyoda, 2016, Regulons of global transcription factors in Corynebacterium glutamicum, Appl Microbiol Biotechnol, 100, 45, 10.1007/s00253-015-7074-3 Cramer, 2006, Identification of RamA, a novel LuxR-type transcriptional regulator of genes involved in acetate metabolism of Corynebacterium glutamicum, J Bacteriol, 188, 2554, 10.1128/JB.188.7.2554-2567.2006 Gerstmeir, 2004, RamB, a novel transcriptional regulator of genes involved in acetate metabolism of Corynebacterium glutamicum, J Bacteriol, 186, 2798, 10.1128/JB.186.9.2798-2809.2004 Park, 2010, Functional characterization of the glxR deletion mutant of Corynebacterium glutamicum ATCC 13032: involvement of GlxR in acetate metabolism and carbon catabolite repression, FEMS Microbiol Lett, 304, 107, 10.1111/j.1574-6968.2009.01884.x Lou, 2012, Ribozyme-based insulator parts buffer synthetic circuits from genetic context, Nat Biotechnol, 30, 1137, 10.1038/nbt.2401 Hsu, 2006, Initial transcribed sequence mutations specifically affect promoter escape properties, Biochemistry, 45, 8841, 10.1021/bi060247u Haenssler, 2009, A game with many players: control of gdh transcription in Corynebacterium glutamicum, J Biotechnol, 142, 114, 10.1016/j.jbiotec.2009.04.007 Larson, 2013, CRISPR interference (CRISPRi) for sequence-specific control of gene expression, Nat Protoc, 8, 2180, 10.1038/nprot.2013.132 Yim, 2016, Development of a new platform for secretory production of recombinant proteins in Corynebacterium glutamicum, Biotechnol Bioeng, 113, 163, 10.1002/bit.25692 Martin, 2005, Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity, Nat Biotechnol, 23, 1308, 10.1038/nbt1136 Haitjema, 2014, Universal genetic assay for engineering extracellular protein expression, ACS Synth Biol, 3, 74, 10.1021/sb400142b Adams, 2002, New biarsenical Ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications, J Am Chem Soc, 124, 6063, 10.1021/ja017687n Zhang, 2019, Development of a secretory expression system with high compatibility between expression elements and an optimized host for endoxylanase production in Corynebacterium glutamicum, Microb Cell Factories, 18, 72, 10.1186/s12934-019-1116-y Silva, 2019, De novo design of potent and selective mimics of IL-2 and IL-15, Nature, 565, 186, 10.1038/s41586-018-0830-7 Teramoto, 2011, High yield secretion of heterologous proteins in Corynebacterium glutamicum using its own Tat-type signal sequence, Appl Microbiol Biotechnol, 91, 677, 10.1007/s00253-011-3281-8 Chatzi, 2017, Preprotein mature domains contain translocase targeting signals that are essential for secretion, J Cell Biol, 216, 1357, 10.1083/jcb.201609022 Sardis, 2017, Preprotein conformational dynamics drive bivalent translocase docking and secretion, Structure, 25, 1056, 10.1016/j.str.2017.05.012 Ragauskas, 2006, The path forward for biofuels and biomaterials, Science, 311, 484, 10.1126/science.1114736 Shah, 2018, The RamA regulon: complex regulatory interactions in relation to central metabolism in Corynebacterium glutamicum, Appl Microbiol Biotechnol, 102, 5901, 10.1007/s00253-018-9085-3 Ingram, 1990, Ethanol tolerance in bacteria, Crit Rev Biotechnol, 9, 305, 10.3109/07388558909036741 Yoon, 2018, CRISPR interference-mediated metabolic engineering of Corynebacterium glutamicum for homo-butyrate production, Biotechnol Bioeng, 115, 2067, 10.1002/bit.26720 Lee, 2018, Rapid identification of unknown carboxyl esterase activity in Corynebacterium glutamicum using RNA-guided CRISPR interference, Enzym Microb Technol, 114, 63, 10.1016/j.enzmictec.2018.04.004