Development of a nanoparticle-assisted PCR assay for detection of bovine respiratory syncytial virus
Tóm tắt
Bovine respiratory syncytial virus (BRSV) is a common pathogen causing respiratory disease in cattle and a significant contributor to the bovine respiratory disease (BRD) complex. BRSV is widely distributed around the world, causing severe economic losses. This study we established a new molecular detection method of BRSV pathogen NanoPCR attributed to the combination of nano-particles in traditional PCR (Polymerase chain reaction) technology. In this study, the BRSV NanoPCR assay was developed, and its specificity and sensitivity were investigated. The results showed that no cross-reactivity was observed for the NanoPCR assay for related viruses, including the infectious bovine rhinotracheitis virus (IBRV), bovine viral diarrhea virus (BVDV), and bovine parainfluenza virus type 3 (BPIV3), and the assay was more sensitive than the conventional PCR assay, with a detection limit of 1.43 × 102 copies recombinant plasmids per reaction, compared with 1.43 × 103 copies for conventional PCR analysis. Moreover, thirty-nine clinical bovine samples collected from two provinces in North-Eastern China, 46.15% were determined BRSV positive by our NanoPCR assay, compared with 23.07% for conventional PCR. This is the first report to demonstrate the application of a NanoPCR assay for the detection of BRSV. The sensitive and specific NanoPCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of BRSV infection.
Tài liệu tham khảo
Van der Poel WH, Brand A, Kramps JA, Van Oirschot JT. Respiratory syncytial virus infections in human beings and in cattle. J Inf Secur. 1994;29(2):215–28.
Griffin D, Chengappa MM, Kuszak J, McVey DS. Bacterial pathogens of the bovine respiratory disease complex. Vet Clin North Am Food Anim Pract. 2010;26(2):381–94.
Larsen LE. Bovine respiratory syncytial virus (BRSV): a review. Acta Vet Scand. 2000;41(1):1–24.
Baker JC, Ellis JA, Clark EG. Bovine respiratory syncytial virus. The Veterinary clinics of North America Food animal practice. 1997;13(3):425–54.
Stott EJ, Taylor G. Respiratory syncytial virus. Brief review. Arch Virol. 1985;84(1–2):1–52.
Shirvani E, Lotfi M, Kamalzadeh M, Noaman V, Bahriari M, Morovati H, Hatami A. Seroepidemiological study of bovine respiratory viruses (BRSV, BoHV-1, PI-3V, BVDV, and BAV-3) in dairy cattle in central region of Iran (Esfahan province). Trop Anim Health Prod. 2012;44(1):191–5.
Willoughby K, Thomson K, Maley M, Gilray J, Scholes S, Howie F, Caldow G, Nettleton PF. Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing. Vet Microbiol. 2008;126(1–3):264–70.
Horwood PF, Mahony TJ. Multiplex real-time RT-PCR detection of three viruses associated with the bovine respiratory disease complex. J Virol Methods. 2011;171(2):360–3.
Li H, Huang J, Lv J, An H, Zhang X, Zhang Z, Fan C, Hu J. Nanoparticle PCR: nanogold-assisted PCR with enhanced specificity. Angew Chem Int Ed Engl. 2005;44(32):5100–3.
Demming A. The state of research after 25 years of nanotechnology. Nanotechnology. 2014, 25(1):010201.
Paccaud MF, Jacquier C. A respiratory syncytial virus of bovine origin. Arch Gesamte Virusforsch. 1970;30(4):327–42.
Rosenquist BD. Isolation of respiratory syncytial virus from calves with acute respiratory disease. J Infect Dis. 1974;130(2):177–82.
Smith MH, Frey ML, Dierks RE. Isolation, characterization, and pathogenicity studies of a bovine respiratory syncytial virus. Arch Virol. 1975;47(3):237–47.
Brodersen BW. Bovine respiratory syncytial virus. Vet Clin North Am Food Anim Pract. 2010;26(2):323–33.
Li M, Lin YC, Wu CC, Liu HS. Enhancing the efficiency of a PCR using gold nanoparticles. Nucleic Acids Res. 2005;33(21):e184.
Boxus M, Letellier C, Kerkhofs P. Real time RT-PCR for the detection and quantitation of bovine respiratory syncytial virus. J Virol Methods. 2005;125(2):125–30.
Thonur L, Maley M, Gilray J, Crook T, Laming E, Turnbull D, Nath M, Willoughby K. One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3. BMC Vet Res. 2012;8:37.
Hazari S, Panda HK, Kar BC, Das BR. Comparative evaluation of indirect and sandwich ELISA for the detection of antibodies to bovine respiratory syncytial virus (BRSV) in dairy cattle. Comp Immunol Microbiol Infect Dis. 2002;25(1):59–68.
Mayers J, Sawyer J. Development and evaluation of a multiplex enzyme-linked immunosorbent assay for the detection of antibodies to bovine respiratory diseases on the Meso scale discovery platform. J Vet Diagn Invest. 2012;24(4):725–9.
Ali Z, Jin G, Hu Z, Wang Z, Khan MA, Dai J, Tang Y. A review on NanoPCR: history, mechanism and applications. J Nanosci Nanotechnol. 2018;18(12):8029–46.
Guo L, Wang W, Zhang SQ, Sun N, FX Wang HW. Establishment of vaccination test for bovine respiratory disease syndrome-associated virus BVDV, BPIV3, infectious rhinotracheitis virus and BRSV. Specialty Res. 2014;36(01):12–6.
Wang J, Cheng Y, Zhang M, Zhao H, Lin P, Yi L, Tong M, Cheng S. Development of a nanoparticle-assisted PCR (nanoPCR) assay for detection of mink enteritis virus (MEV) and genetic characterization of the NS1 gene in four Chinese MEV strains. BMC Vet Res. 2015;11(1).