Development of a Self‐Assembled Peptide/Methylcellulose‐Based Bioink for 3D Bioprinting

Macromolecular Materials and Engineering - Tập 304 Số 11 - 2019
Carla Cofiño1,2, Soledad Pérez‐Amodio1,3,3, Carlos E. Semino2, Elisabeth Engel1,3,3, Miguel A. Mateos‐Timoneda1,3,3
1Biomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, 08028 Barcelona, Spain
2Department of Bioengineering, IQS-School of Engineering, Ramon Llull University, 08017 Barcelona, Spain
3Department of Material Science and Metallurgical Engineering EEBE campus Technical University of Catalonia (UPC) 08019 Barcelona Spain

Tóm tắt

AbstractThe introduction of 3D bioprinting to fabricate living constructs with tailored architecture has provided a new paradigm for biofabrication, with the potential to overcome several drawbacks of conventional scaffold‐based tissue regeneration strategies. Hydrogel‐based materials are suitable candidates regarding cell biocompatibility but often display poor mechanical properties. Self‐assembling peptides are a promising source of biomaterials to be used as 3D scaffolds based on their similarity to extracellular matrices (structurally and mechanically). In this study, an advanced bioink for biofabrication is presented based on the optimization of a RAD16‐I‐based biomaterial. The strategy followed to build 3D predefined structures by 3D printing is based on an enhancement of bioink viscosity by adding methylcellulose (MC) to a RAD16‐I solution. The resultant constructs display high shape fidelity and stability and embedded human mesenchymal stem cells present high viability after 7 days of culture. Moreover, cells are also able to differentiate to the adipogenic lineage, suggesting the suitability of this novel biomaterial for soft tissue engineering applications.

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Macromolecular Materials and Engineering

Tập 304 Số 11

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