Development of IVM‐IVF produced 8‐cell bovine embryos in simple, serum‐free media after conditioning or Co‐culture with buffalo rat liver cells

Molecular Reproduction and Development - Tập 38 Số 3 - Trang 251-255 - 1994
Najeeb Ur Rehman1, Andrew Collins1, T.K. Suh1, R.W. Wright1
1Department of Animal Science, Washington State University, Pullman, Washington

Tóm tắt

AbstractDevelopment of 8‐cell bovine embryos derived from in vitro matured/in vitro fertilized (IVM/IVF) oocytes was evaluated in two simple, serum‐free media (CZB and SOM) with buffalo rat liver cells co‐culture (BRLC) or after conditioning compared to a commonly used, serum‐supplemented complex medium TCM‐199. In a 3 x 4 factorial design, 578 eight‐cell embryos were randomly assigned to 12 treatment groups. The factors were: first, type of culture medium (M199/FBS, CZBg and SOM), and second, the use of BRLC (as co‐culture or to condition media for 24 hr and 48 hr) and unconditioned media. Development to morula was not affected by the type of medium, but co‐culture and 48 hr conditioning within media type resulted in better development when compared to the 24‐hr conditioned or unconditioned groups. Blastocyst development in SOM (38.9%) was different (P < 0.05) than in CZBg (46.6%) and M199/FBS (48.7%) and was lowest in the unconditioned group (27.8%) followed by 24 hr conditioned (33.3%), 48 hr (56.3%), and co‐culture (59.6%). No blastocyst expansion was observed with unconditioned media and 24 hr conditioned SOM. Significant differences (P < 0.05) were found among all treatment groups except the co‐culture and 48‐hr conditioned groups. Hatching occurred only with co‐culture and 48‐hr conditioned groups of M199/FBS and CZBg media. These data show that CZB with glucose conditioned by BRLC monolayers for 48 hr can support the development of IVM/IVF produced bovine embryos to blastocyst compared to culture in TCM‐199 with serum. © 1994 Wiley‐Liss, Inc.

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Molecular Reproduction and Development

Tập 38 Số 3

251-255

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