Development of 19 polymorphic microsatellite loci for the intermediate horseshoe bat, Rhinolophus affinis (Rhinolophidae, Chiroptera)
Tóm tắt
The intermediate horseshoe bat (Rhinolophus affinis) is a widespread species in Southeast Asia. We developed 19 novel microsatellite loci from an enriched genomic library of the bat, and tested their polymorphism using a single population from Guangdong province, southern China. The number of alleles ranged from 3 to 15 per locus with the expected and observed heterozygosity ranging from 0.397 to 0.920 and 0.280–0.926, respectively. Three markers significantly deviated from the Hardy-Weinberg expectations after Bonferroni correction and no linkage disequilibrium was detected in any of loci. These microsatellite loci will be useful in studying the phylogeography of this species.
Tài liệu tham khảo
Csorba G, Ujhelyi P, Thomas N (2003) Horseshoe bats of the world (Chiroptera: Rhinolophidae). Alana Books, Shropshire, UK, 118 Rouge
Duckworth JW, Salter RE, Khounboline K (1999) Wildlife in Lao PDR: 1999 Status Report
Hua PY, Chen JP, Sun M, Liang B, Zhang SY, Wu DH (2006) Characterization of microsatellite loci in fulvous fruit bat Rousettus leschenaulti. Mol Ecol Notes 6:939–941. doi:10.1111/j.1471-8286.2006.01407.x
Karp A, Isaac PG, Ingram DS (1998) Molecular tools for screening biodiversity. Chapman & Hall, London
Koopman KF (1994) Chiroptera: systematics. Handbook of Zoology. Walter de Gruyter, Berlin. Mammalia, part 60
Raymond M, Rousset F (1995) GENEPOP (version 1.2)—population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Simmons NB (2005) Order Chiroptera. In: Wilson DE, Reeder DM (eds) Mammal species of the world: a taxonomic and geographic reference. Johns Hopkins University Press, Baltimore, pp 312–529
Van Oosterhout C, Hutchinson WF, Wills DPM et al (2004) MICROCHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538