Development and evaluation of a real-time quantitative PCR for the detection of human cytomegalovirus

Aitken, 1999, Use of molecular assays in diagnosis and monitoring of cytomegalovirus disease following renal transplantation, J. Clin. Microbiol., 37, 2804, 10.1128/JCM.37.9.2804-2807.1999 Bernard, 1998, Integrated amplification and detection of the C677T point mutation in the methylenetetrahydrofolate reductase gene by fluorescence resonance energy transfer and probe melting curves, Anal. Biochem., 255, 101, 10.1006/abio.1997.2427 Boom, 1999, A highly sensitive assay for detection and quantitation of human cytomegalovirus DNA in serum and plasma by PCR and electrochemiluminescence, J. Clin. Microbiol., 37, 1489, 10.1128/JCM.37.5.1489-1497.1999 Cane, 1999, Use of real-time PCR and fluorimetry to detect Lamivudine resistance-associated mutations in Hepatitis B virus, Antimicrob. Agents Chemother., 43, 1600, 10.1128/AAC.43.7.1600 Darlington, 1991, Use of the polymerase chain reaction to analyse sequence variation within a major neutralising epitope of glycoprotein B (gp58) in clinical isolates of human cytomegalovirus, J. Gen. Virol., 72, 1985, 10.1099/0022-1317-72-8-1985 Emery, 2000, Application of viral-load kinetics to identify patients who develop cytomegalovirus disease after transplantation, Lancet, 355, 2032, 10.1016/S0140-6736(00)02350-3 Fox, 1995, Longitudinal analysis of cytomegalovirus load in renal transplant patients using a quantitative polymerase chain reaction: correlation with disease, J. Gen. Virol., 76, 309, 10.1099/0022-1317-76-2-309 Fryd, 1980, Cytomegalovirus as a risk factor in renal transplantation, Transplantation, 30, 436, 10.1097/00007890-198012000-00010 Gor, 1998, Longitudinal fluctuations in cytomegalovirus load in bone marrow transplant patients: relationship between peak virus load, donor/recipient serostatus, acute GVHD and CMV disease, Bone Marrow Transplant., 21, 597, 10.1038/sj.bmt.1701139 Guiver, M., Fox, A.J., Mutton, K., Mogulkoc, N., Egan, J. (in press). Comparative evaluation of CMV viral load using TaqMan CMV quantitative PCR with CMV antigenaemia in heart and lung transplant recipients. Transplantation. Jacobson, 1988, Serious cytomegalovirus disease in the acquired immune deficiency syndrome (AIDS), Ann. Intern. Med., 108, 585, 10.7326/0003-4819-108-4-585 Kanj, 1996, Cytomegalovirus infection following liver transplantation: Review of the literature, Clin. Infect. Dis., 22, 537, 10.1093/clinids/22.3.537 Machida, 2000, Real-time automated PCR for early diagnosis and monitoring of cytomegalovirus infection after bone marrow transplantation, J. Clin. Microbiol., 38, 2536, 10.1128/JCM.38.7.2536-2542.2000 Mustafa, 1994, Cytomegalovirus infection and disease in the immunocompromised host, Pediatr. Infect. Dis. J., 13, 249, 10.1097/00006454-199404000-00001 Nitsche, 1999, Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA, Clin. Chem., 45, 192, 10.1093/clinchem/45.11.1932 Nitsche, 2000, Detection of human cytomegalovirus DNA by real-time quantitative PCR, J. Clin. Microbiol., 38, 2734, 10.1128/JCM.38.7.2734-2737.2000 Pellegrin, 1999, Evaluation of new quantitative assays for diagnosis and monitoring of cytomegalovirus disease in human immunodeficiency virus-positive patients, J. Clin. Microbiol., 37, 3124, 10.1128/JCM.37.10.3124-3132.1999 Sia, 2000, Evaluation of the COBAS AMPLICOR CMV MONITOR test for detection of viral DNA in specimens taken from patients after liver transplantation, J. Clin. Microbiol., 38, 600, 10.1128/JCM.38.2.600-606.2000 Smyth, 1991, Cytomegalovirus infection in heart-lung transplant recipients: Risk factors, clinical associations, and response to treatment, J. Infect. Dis., 164, 1045, 10.1093/infdis/164.6.1045 Tanaka, 2000, Quantitative analysis of cytomegalovirus load using real-time PCR assay, J. Med. Virol., 60, 455, 10.1002/(SICI)1096-9071(200004)60:4<455::AID-JMV14>3.0.CO;2-Q Weinberg, 2000, Comparison of PCR, antigenaemia assay, and rapid blood culture for detection and prevention of cytomegalovirus disease after lung transplantation, J. Clin. Microbiol., 38, 768, 10.1128/JCM.38.2.768-772.2000 Winston, 1990, Cytomegalovirus infections after allogenic bone marrow transplantation, Rev. Infect. Dis., 12, S776, 10.1093/clinids/12.Supplement_7.S776 Yun, 2000, Real-time monitoring of cytomegalovirus infections after stem cell transplantation using the TaqMan polymerase chain reaction assays, Transplantation, 69, 1733, 10.1097/00007890-200004270-00037